Operation Manual

PRINCIPLE

Fluorescence

The phenomenon that occurs when light absorbed by a material, creates a molecular excitation that

causes the material to re-emit energy in the form of light at a different wavelength.

Fluorescence Microscope for Epi-Fluorescence

The technique of fluorescence microscopy with Epi-illuminators is based on the adaptation ofthe vertical

illuminator used in reflected light microscopy. The radiation emitted by the lightsource first passes

through the exciting filter and is incident to a short-pass filter* (dichroic beamsplitter). The radiation is

then reflected into the objective, which also serves as a condenser.The objective concentrates the

exciting radiation in the object field. Fluorescent light emittedfrom there is collected by the objective and

returned in the opposite direction to the short-passfilter, which directs it into the eyepiece through the

barrier filter.

* A filter designed to allow the passage of radiation of wavelengths shorter than a given limit.

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permission.