Specification Sheet

Innovative Bioanalysis LLC. WAC LIGHTING UVF7IN-120V-R1-MOD AEROSOL Page 9 of 11
VIRAL STOCK: SARS-CoV-2 USA-CA1/2020 (BEI NR-52382)
TEST
RESULTS
Identification by Infectivity in Vero 6
cells
Cell Rounding and
Detachment
Next Generation Sequencing (NGS) of
complete genome using Illumina®
iSeq™ 100 Platform
(Approx. 940 Nucleotides
99.9% identity with SARS-
CoV 2, isolate USA-CA1/2020
GenBank: MN994467.1
100% identity with SARS-CoV
2, strain FDAARGOS_983
isolate USA-CA1/2020
GenBank: MT246667.1
Titer by TCID50 in Vero E6 Cells by
Cytopathic effect
2.8 X 10^5 TCID50 per mL in
5 days at 37°C and 5% CO2
Sterility (21-Day Incubation)
Harpos HTYE Broth, aerobic
Trypticase Soy Broth, aerobic
Sabourad Broth, aerobic
Sheep Blood Agar, aerobic
Sheep Blood Agar, anaerobic
Thioglycollate Broth, anaerobic
DMEM with 10% FBS
No Growth
No Growth
No Growth
No Growth
No Growth
No Growth
No Growth
Sterility (21-Day Incubation)
Harpos HTYE Broth, aerobic
Trypticase Soy Broth, aerobic
Sabourad Broth, aerobic
Sheep Blood Agar, aerobic
Sheep Blood Agar, anaerobic
Thioglycollate Broth, anaerobic
DMEM with 10% FBS
No Growth
No Growth
No Growth
No Growth
No Growth
No Growth
No Growth
Mycoplasma Contamination
Agar and Broth Culture
DNA Detection by PCR of extracted
Test Article nucleic acid.
None Detected
None Detected
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