Specification Sheet

Innovative Bioanalysis LLC. WAC LIGHTING UVF7IN-120V-R1-MOD AEROSOL Page 8 of 11
CONTROL:
One Control test was conducted without the UV-C fan system activated in the testing chamber.
Control samples were taken at each of the corresponding sample times for the viral challenge.
Nebulization of viral media was the same for the control as the viral challenge. Control testing was used
for the comparative baseline to assess the viral reduction when the UV system was operated in the
challenge trials, to enable net reduction calculations to be made. During the control test, four low
volume fans were operated in each corner of the testing chamber to ensure homogenous mixing of the
pathogen and shut off at the start of the trial which would be indicated by the 0-time point. During the
control temperature and relative humidity were monitored. Prior to running the viral challenges
temperature and humidity were confirmed to be in relative range to the control +/- 3%.
VIRAL CHALLENGE:
The challenge pathogen, SARS-CoV-2-USA-CA1/2020, was used for testing the efficacy of the fan
system equipped with the UV-C ring technology. During the challenge tests the pressure in the
challenge chamber was monitored to confirm no portion of the chamber was leaking. The bioaerosol
efficacy challenge was completed in three distinct trials with the active pathogen to create a baseline of
data. The UV-C fan combination system was in the same position for each viral challenge and operated
in the same manner. Prior to nebulizing the viral pathogen, the UV system was turned on and allowed
to run for 15 minutes to simulate a real-world environment and allow the device to reach standard
operating conditions and turned off just prior to starting nebulization. Four low volume mixing fans
were used during the nebulization period of the control test and viral pathogen test. Sample times were
as follows with T equal to minutes, T-20, T-30, T-40. Sampling occurred using 2 automatic air volume
samplers that operated simultaneously for each collection. Samplers were pre-set to automatically shut
off after 10 minutes of collection. Collections were made via the equipment utilizing viral media coated
filters for maximum pathogen trapping and stability. Collection samples were provided to lab staff for
pooling after each collection time point.
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