Specification Sheet

Innovative Bioanalysis LLC. WAC LIGHTING UVF7IN-120V-R1-MOD AEROSOL Page 6 of 11
BIOAEROSOL SAMPLING:
For air sampling 2 different Gillian 10i programmable vacuum devices were used. Air samplers
were calibrated by the manufacturer in September 2020 and certificates were inspected prior to use.
Air sample volume collections were confirmed prior to use with a Gilian Gilibrator 2 SN- 200700-12 and
a high flow bubble generator SN-2009012-H. Air samplers were operated in conjunction with removable
sealed cassettes, which were manually removed after each sampling time point. Cassettes had a
delicate internal filtration disc to collect viral samples. The low volume mixing fans stayed active
throughout all testing scenarios and conditions.
VIRUS STRAIN BACKGROUND:
The following reagent was deposited by the Centers for Disease Control and Prevention and
obtained through the BEI Resources, BIAID, NIH SARS-Related Coronavirus 2, Isolate USA-CA1/2020, NR-
52382. This was the chosen pathogen strain because it was taken from a 38-year-old subject with
severe acute respiratory syndrome in California as was part of the A lineage. This was a non-fatal case
which represents most of the cases in the United State. The age group the patient belonged to was not
elderly or juvenile, which was determined to be a good medium average.
POST DECONTAMINATION:
At the conclusion of each viral challenge timepoint/ test the UV system inside the testing
chamber was activated for 30 minutes. After 30 minutes of UV exposure the chamber was fogged with
a Hydrogen Peroxide gas mixture followed by a 30-minute air purge. All test equipment was cleaned at
the end of each day with a 70% alcohol solution. Collection lines were soaked in a bleach bath mixture
for 30 minutes then rinsed repeatedly with DI water. Nebulizer and Vacuum collection pumps were
decontaminated with Hydrogen Peroxide mixtures.
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