User Guide
8 • Miro Canvas • User Guide
• Instrument Loading
i. Power up the Miro Canvas by pressing the black power button on the back of the instrument.
ii. Follow the directions on the touchscreen to load the instrument.
1. If necessary, rst use Miro Palette to send the protocol to the instrument.
2. On the instrument touchscreen, select the user and the desired protocol.
iii. Open the instrument lid by securing the base of the instrument with one hand, grasp the front center
of the lid with the other hand, and lift straight up. Release the cartridge clamp and load the new, empty
Miro Cartridge into the instrument. Lower the cartridge clamp until the clip engages.
IMPORTANT: Do not set the cartridge on the lab bench.
IMPORTANT: Do not touch the bottom lm of the cartridge.
IMPORTANT: Do not slide the cartridge on the electrode board.
• Reagent Loading
i. Load the prepared reagents into the cartridge inlets, as indicated on the touchscreen.
1. Pulse spin the reagents to bring all contents to the bottom of the tube.
2. Set a 200 μl pipette to 20 μl greater than the collective volume of the reagent and Dropgloss, and draw
liquid up from the bottom of the PCR tube. Drawing reagent+Dropgloss into the pipette tip in this
manner ensures that any small volume of liquid left in the PCR tube is Dropgloss, which will not
negatively affect the run.
IMPORTANT: For Reaction Mixes containing
>50 µl Dropgloss, place the pipette tip in the
top layer (Dropgloss) of liquid and draw up
as much as possible, then lower the tip to the
bottom of the tube and draw up the remaining
liquid from the bottom.
IMPORTANT: Use standard 200 μl barrier pipette
tips when loading inlets. Smaller pipette tips,
when inserted fully, will touch and could puncture
the lm on the bottom of the cartridge. Any
resulting lm deformations can negatively affect the run,
and a puncture in the lm could compromise the Miro Canvas.
3. To load, fully insert the pipette tip into the inlet, then depress the plunger in a single, swift, and smooth
motion until you reach the rst stop of the pipette. Hold the depressed pipette in the inlet for 3
seconds. Remove the pipette tip from the inlet before releasing the plunger. Repeat for each reagent.
IMPORTANT: Air in the pointed end of the pipette tip is normal and will harmlessly dissipate into the
air within the cartridge. However, depressing the pipette past the rst stop can introduce an air
bubble into the reagent droplet, which can negatively affect the run.
4. It may take a moment for the reagent to be detected once loaded into the cartridge inlet. Once
detected, the touchscreen will automatically prompt you to load the next reagent.
5. Once all inlet reagents are loaded, press Continue on the touchscreen to advance to reservoir loading.
ii. Load the prepared reagents into the reservoirs, as indicated on the touchscreen.
a. Remove the blue cap from the reservoir. To load, draw up the reagent using a 1000 ul pipette tted
with a barrier tip. Hold the pipette vertically and fully insert it into the reservoir to dispense. Remove
the tip from the reservoir before releasing the pipette plunger.