User Guide

iii. Power up the instrument by pressing the black power button on the back of the Canvas. The LED
indicator bar will change to blue as the instrument initializes.
1. The instrument may take a few minutes to power up.
iv. Use the touchscreen Settings menu to:
1. Connect to a network
a. To use an Ethernet connection (recommended for stability), plug in an Ethernet cord and toggle
off Wi-Fi on the touchscreen Settings menu.
b. To use a Wi-Fi connection, connect the Wi-Fi antenna, toggle on Wi-Fi on the touchscreen Settings
menu, and ensure that an Ethernet cord is not plugged into the instrument.
c. Network connectivity is not required to run existing protocols, but is critical for running new
protocols, software and rmware updates, and optimal troubleshooting support.
2. Name your Canvas
3. Level the instrument
a. Lift the instrument lid, pull the cartridge clip toward you to release the cartridge clamp. Lift the clamp
straight up and out of the way.
b. Gently place the leveling plate on top of the electrode board. Twist the feet left and right, one at a
time, to raise and lower the instrument until the bubble is in the center of the leveling plate.
c. During a run, when a cartridge is loaded in the instrument, the leveling plate can be stored in the
clean, empty cartridge packaging. When the instrument is not in use, the leveling plate can be
stored inside the instrument to protect the electrode board. Ensure that the leveling plate stays clean
to avoid contaminating or scratching the electrode board.
4. Run Miro Diagnostics (optional)
a. Miro Diagnostics tests the core functionality of the instrument. Miro Diagnostics can be run to
increase condence prior to running a protocol with a precious sample, or to aid in troubleshooting
after a protocol has behaved unexpectedly. It is good practice to run Miro Diagnostics weekly.
Section 6 • Operation
1. REQUIRED MATERIALS
• Reagents
i. Reagents provided with desired library preparation kit or custom workow, including adapters
ii. Additional reagents, as required for desired workow (nuclease-free water, fresh 80% ethanol,
10 mM Tris-HCl, etc.)
iii. Miro Dropgloss
• Equipment
i. Miro Canvas instrument
ii. Uninterruptible power supply (recommended)
iii. Miro Cartridge
iv. Additional equipment, as required for desired workow
6 • Miro Canvas • User Guide