Technical data

Measuring Samples
Agilent MicroLab Software Operation Manual 35
Collecting the sample measurement
To measure a sample:
1 To begin sample measurement, click the Start button on the
Home screen.
2 As the software instructs, clean the sample interface for an ATR
or Transmission (Agilent 4500t/5500t FTIR) measurement. For a
reflectance measurement on the Agilent 4100 ExoScan FTIR, the
reflection standard should be placed on the sample interface.
NOTE
The sample surface should be cleaned with a soft tissue and an appropriate
solvent such as methanol, ethanol or acetone.
3 Click the Next button. Clicking the Home button will stop the
measurement and return you to the Home screen.
The Clean Crystal Check will be conducted if it has been selected
in the method. This will check for absorbance over a stored
reference. If any absorbances are found, the software will
instruct you to clean the sample interface and start again.
NOTE
If the clean crystal check continually fails, the stored background scan may be
corrupted. If this occurs, the clean background can be reset by clicking Advanced
Features > Diagnostics then Reset Clean Check in the Diagnostics dialog box.
This will clear the stored background.
4 Following the crystal check (if specified), the software will collect
the background sample. Background samples are collected either
at every scan or after a specified time interval, as specified in the
method.
The software will instruct you to apply the sample to the sample
interface. The picture displayed will match the configuration of
the system. Once the sample is in place, click Next.