Technical data

MicroLab PC
26 Agilent MicroLab Software Operation Manual
Recommend page
The recommendations will be displayed on the Results screen
following sample analysis. The messages are a unique feature; they
give the user the ability to customize results to their specific needs
and parameters.
Univariate component calibration
MicroLab PC software contains functionality to implement a
linear calibration and conduct a quantitative prediction;
however, MicroLab PC does not have facilities to create a linear
fit to collected data in order to perform this calibration.
To conduct linear calibrations:
1 Using a Data Collect method, collect representative spectra of
high and low concentration samples in the appropriate sample
matrix.
2 View the spectra to determine peaks that can be used to predict
the concentration. In general, look for peaks that increase with
concentration and are isolated from interfering peaks.
3 Create a new Component method using the collection parameters
(resolution, number of scans, and so on) desired for the final
analysis.
4 On the Component page, click the Add button to define a new
component. Select the desired type of calculation (Peak Height,
Peak Area, and so on) and define the peaks and baseline points
determined in Step 2.
5 Select an appropriate scaling factor (10, 100, 1000) to ensure that
the measurements of the peak height or area are whole numbers.
Choose that the result be listed as an Actual Value.
6 Measure samples over the desired concentration range using this
newly defined component method. Record the results listed for
the defined component.
7 Enter the component results measured in Step 6 along with the
concentrations associated with those results into a commercially-
available spreadsheet software package, such as Microsoft Excel.