Certifications 2

SDS No.: 370156 V003.0
Metylan Ovalit P
Page 7 of 12
Respiratory or skin sensitization:
The mixture is classified based on threshold limits referring to the classified substances present in the mixture.
Hazardous substances
CAS-No.
Result
Test type
Species
Method
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
sensitising
Guinea pig maximisation
test
guinea pig
OECD Guideline 406 (Skin Sensitisation)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
sensitising
Mouse local lymphnode
assay (LLNA)
mouse
not specified
Germ cell mutagenicity:
The mixture is classified based on threshold limits referring to the classified substances present in the mixture.
Hazardous substances
CAS-No.
Result
Type of study /
Route of
administration
Metabolic
activation /
Exposure time
Species
Method
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
ambiguous
bacterial reverse
mutation assay (e.g
Ames test)
with and without
equivalent or similar to OECD
Guideline 471 (Bacterial
Reverse Mutation Assay)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
positive
in vitro mammalian
chromosome
aberration test
with and without
EPA OPP 84-2 (Mutagenicity
Testing)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
positive
mammalian cell
gene mutation assay
with and without
OECD Guideline 476 (In vitro
Mammalian Cell Gene
Mutation Test)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
negative
DNA damage and
repair assay,
unscheduled DNA
synthesis in
mammalian cells in
vitro
not applicable
OECD Guideline 482 (Genetic
Toxicology: DNA Damage
and Repair, Unscheduled
DNA Synthesis in Mammalian
Cells In Vitro)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
negative
oral: gavage
mouse
OECD Guideline 474
(Mammalian Erythrocyte
Micronucleus Test)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
negative
oral: gavage
mouse
OECD Guideline 475
(Mammalian Bone Marrow
Chromosome Aberration Test)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
negative
oral: feed
Drosophila
melanogaster
OECD Guideline 477 (Genetic
Toxicology: Sex-linked
Recessive Lethal Test in
Drosophila melanogaster)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
negative
oral: gavage
rat
OECD Guideline 486
(Unscheduled DNA Synthesis
(UDS) Test with Mammalian
Liver Cells in vivo)
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
negative
oral: gavage
rat
EPA OPP 84-2 (Mutagenicity
Testing)
Carcinogenicity
The mixture is classified based on threshold limits referring to the classified substances present in the mixture.
Hazardous components
CAS-No.
Result
Route of
application
Exposure
time /
Frequency
of treatment
Species
Sex
Method
Isothiazolinone mixture
3:1 (CIT/MIT)
55965-84-9
not carcinogenic
oral: drinking
water
2 y
daily
rat
male/female
OECD Guideline 453
(Combined Chronic
Toxicity /
Carcinogenicity
Studies)