CH-9101 Internet E-Mail Herisau/Switzerland www.metrohm.com info@metrohm.com 6.6034.023 Metrodata IC Net 2.2 8.110.8263 Software Manual 02.
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Table of contents Table of contents 1 Introduction ...............................................................1 1.1 1.2 1.3 1.4 2 Fundamentals of the operation ................................7 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 3 3.3 3.4 Fonts ........................................................................................ 15 Security system....................................................................... 15 3.2.1 Security Options window...........................................
Table of contents 4.2 4.3 4.4 4.5 5 Interfaces ................................................................36 5.1 5.2 5.3 5.4 5.5 6 Interface installation ............................................................... 36 5.1.1 Add interfaces with system wizard ............................... 36 5.1.2 Add interfaces in workplace window............................ 37 5.1.3 Delete interfaces........................................................... 37 762 IC Interface............................
Table of contents 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 6.10 6.11 6.12 6.13 IC Net 2.2 Watch window ......................................................................... 52 6.2.1 Watch window icon ...................................................... 52 6.2.2 Watch window settings................................................. 52 732 IC Detector ........................................................................ 54 6.3.1 732 IC Detector features ...........................................
Table of contents 6.14 6.15 6.16 6.17 6.18 6.19 6.20 7 Methods .................................................................142 7.1 7.2 7.3 7.4 IV 6.13.3 812 Valve Unit window................................................ 107 816 IC Eluent Selector ...........................................................109 6.14.1 816 IC Eluent Selector features .................................. 109 6.14.2 816 IC Eluent Selector icon ........................................ 109 6.14.
Table of contents 7.5 7.6 8 Chromatograms.....................................................188 8.1 8.2 8.3 8.4 8.5 8.6 IC Net 2.2 Calibration and quantification.............................................. 161 7.5.1 General information .................................................... 161 7.5.2 Notations .................................................................... 162 7.5.3 External standard calibration...................................... 163 7.5.4 Component table..................
Table of contents 8.6.7 8.6.8 9 Invert chromatogram .................................................. 207 Autodatabase ............................................................. 207 Sample queue........................................................209 9.1 9.2 9.3 Sample queue file handling...................................................209 9.1.1 Open sample queue................................................... 209 9.1.2 Save sample queue....................................................
1.1 Purpose of program 1 Introduction 1.1 Purpose of program «IC Net 2.2» is the name of the data acquisition and control software for PC-controlled ion chromatographic systems consisting of Metrohm and Bischoff instruments. The current version is a true 32-bit application. It was designed specially to operate under Windows 2000 and Windows XP and uses all its benefits. The «IC Net 2.2» program can be used to create systems for recording and evaluating chromatograms.
1 Introduction 1.2 Information about the software manual This 8.110.8263 Software Manual provides a comprehensive overview of the operation of the «IC Net» program.
1.3 Notation and pictograms can be updated at any time by downloading the latest applications from the Internet under «www.metrohm.com». Last, but not least, you will find detailed information on the separating columns available from Metrohm and on special IC applications in the relevant "Application Bulletins". 1.
1 Introduction 1.4 Installation 1.4.1 Hardware requirements for the PC 1.4.2 Computer Pentium III with 450 MHz or higher Operating system Windows® 2000 or Windows® XP Free space on hard disk 12 MB for program files 50 MB recommended for data files Working memory RAM 128 MB for Windows® 2000 256 MB for Windows® XP Graphics resolution 800×600, better 1024×768 or more Printer Any printer supported by the operating system Software Installation To use the 21 CFR Part 11 features of «ICNet 2.
1.4 Installation To change from an IC Net installation (without 21 CFR part 11 features) to an IC Net Compliant (with 21 CFR part 11 features) installation (and back) you have to remove the previous installation! Pay attention to the corresponding dialog in the setup program. The two software packages «IC Net 2.2» and «Autodatabase 1.0» will be installed in the desired directory. Icons are created in the program folder, in the startup folder, and on the desktop.
1 Introduction Metrohm IC Net 2.1 in the list and clicking on . All program files and icons should be removed. 5. Delete the folder IC Net 2.1 in the program folder. 6. Install the IC Net 2.2 software (see section 1.4.2). 7. Copy the IC Net 2.1 backup files of the folders Data, Methods, and Systems into the folders ..\IC Net 2.2\IC Net\Data, ..\IC Net 2.2\IC Net\Methods, and ..\IC Net 2.2\IC Net\Systems. 8.
2.1 Starting/closing the program 2 Fundamentals of the operation 2.1 Starting/closing the program 2.1.1 Start the «IC Net» program Start the program Double-click this icon or the ICNet.exe file to start the «IC Net 2.2» program. The IC Net login window appears: Enter your User name and your Password and click on . After software installation at the first start of the software, the Add User window opens (see section 3.2.1)and a user with Administrator access level is created.
2 Fundamentals of the operation 2.2 Glossary Batch reprocessing Batch reprocessing is understood to be the subsequent reprocessing of a series of chromatograms which have been loaded in a batch reprocessing queue. During reprocessing with a selected method the settings for calibration, integration, passport, appearance and report can be altered at will (for details, see section 10).
2.2 Glossary fluenced by the integration parameters and the optionally programmable integration events which are defined in the method. In addition, the integration can be manually corrected later with the aid of the peak editor (see section 8.4), for details see section 7. Interface The «IC Net» program supports remote control for interfaces available from Metrohm and Bischoff. Interfaces generally convert analog signals to digital form, which can be handled by the computer.
2 Fundamentals of the operation 2.3 Overview of program windows «IC Net 2.2» consists of different windows whose functionality is linked together. The different windows are: 2.4 IC NET Main program window with menus for file administration, printing, method modification, options, login and user rights, window handling. CHROMATOGRAM Chromatogram window for graphic plot of running or recorded chromatograms. SYSTEM System window for control of interfaces and devices.
2.5 Icons of the main window 2.5 Icons of the main window The following icons are displayed in the IC NET main window: Open chromatogram Save chromatogram Open last batch reprocessing file Make report Print preview Send chromatograms to Autodatabase Print chromatogram Passport Method setup Integration Components Cascade all opened chromatogram windows Vertical tiling of open chromatogram windows Horizontal tiling of open chromatogram windows Appearance Enable/disable peak editor mode.
2 Fundamentals of the operation 2.6 Overview of file types The following file types are produced by the «IC Net» software: 12 *.adb Autodatabase file Contains chromatogram and method data of all determinations. *.bar Batch reprocessing file (binary file) Contains batch reprocessing data. The *.bar file is stored automatically in the Data folder. *.cal Calibration file (binary file) Contains calibration data, which can be exported with IC Net / Method / Calibration / Export calibration. The *.
2.7 Context-sensitive menus 2.7 *.rtt Report file (ASCII file) Contains a report template. The *.rtt file is stored in the program folder. *.smt System file (ASCII file) Contains the system settings. The *.smt file is stored automatically in the Systems folder. *.dev Device file (ASCII file) Contains drivers for devices. The *.dev file is stored in the Devices folder.
2 Fundamentals of the operation Shifts a chromatogram on 1/10 part of a screen downwards. [ Shift ] + [ up ] Increases a distance between channels of a chromatogram. [ Shift ] + [ down ] Reduces a distance between channels of a chromatogram. [ 0 (Zero) ] Adjusts a zero on the last point of a chromatogram (running chromatogram) or its lowest level (finished run). Only part of the chromatogram is on screen: [ Ctrl ] + [ right ] Moves one window right (without change of scale on X and Y axes).
3.1 Fonts 3 Software settings 3.1 Fonts IC NET / Options / Fonts This option allows the selection of fonts used by the system. Font for dialogs... Selection of font used for dialog boxes. Default setting: MS Sans Serif / Standard / 8 pt. Font for reports... Selection of font used for report output to the screen or printer. Default setting: Courier New / Standard / 10 pt. Font for tables... Selection of font used for data presentation in tables on the screen.
3 Software settings User Window Possible actions Novice, Master New Password This window allows the user to change his Password. Administrator Security options 3.2.1 This window allows editing the User List and the Password Options, it is also possible for an administrator user to change his Password. Security Options window IC NET / Options / Security This window contains the user administration and password options. Only a user with Administrator access level can open it.
3.2 Security system Edit current user's settings (username, full name, access level, status), opens the Modify User window. Change the password of the administrator logged in. Opens the New Password window. Add/Modify User User User name of the user. Full Name Full name of the user, this name is displayed in the status bar of the main window throughout an IC Net session. Level Access level of the user. Restricted access to program functions.
3 Software settings failed Login attempts exceeds the limit set in the Password Options. The user can be set Active again by the Administrator. Removed The User is not able to work with «IC Net», he is removed from the system but he is still listed in the User List. The user can be set Active again by the Administrator. Password Options This tab allows to specify Password and Login related security settings. Minimum Password Length Minimum number of characters for the Password. Range: 2...10, default 6.
3.3 Audit Trail Automatic Logout After After the specified period of inactivity (in minutes) the system is locked, to protect it against unauthorized access. Range: 1,...999, default 30. The program prompts for username and password every time the system starts. This username stamps methods, chromatograms and reports, created during the working session. During the work it is possible to change the current user with the menu item IC NET / Options / Lock system. 3.
3 Software settings Audit Trail window The Audit Trail window displays the Audit Trail records of the IC Net or Chromatogram History in a table. Easy access to specific information is enabled by several sort and filter options of the log window. The Log Window can be sorted according to one of the six columns by clicking the title. A star ( * ) at the end of the title indicates the sorting criterion. Refresh window, not available for Chroma- togram History.
3.4 Global settings Sort window This window offers a more refined possibility to arrange log data by combining the column titles of the Audit Trail window to a Sort Sequence where the Audit Trail records are sorted. Sort Sequence Available Sort criteria of the Sort Sequence, the sort criteria are applied in the order of their entry in the list. Unused sort criteria. Select marked sort criterion. Deselect marked sort criterion. 3.
3 Software settings If method changed Don’t ask to save Ask to save Global settings for saving of method files: The method file is not saved automatically. It can be saved only with File / Save / Method. The user is asked if the method should be saved. Overwrite without asking Method files are overwritten without confirmation. If disk method is newer Ask to overwrite Global settings for overwriting of method files: The user is asked if the method should be overwritten.
3.4 Global settings togram is saved. If this option is disabled, the directory is opened where the last opened chromatogram is saved. 21 CFR Part 11 On Switch on/off global 21 CFR Part 11 settings. Is automatically activated and cannot be altered, if IC Net Compliant is installed.
4 Systems 4 Systems The combination of Metrohm and Bischoff devices connected to an interface is called a system. The system includes all the settings of the devices, their time program, the data acquisition parameters and the processing method which have been optimized for the specific separating column and the determination to be carried out with it. A system is used to start single determinations or determinations with the help of a sample queue. Systems are stored as system files (*.
4.1 System creation , the interface icon will appear on the toolbar (PC Boards will not appear on toolbar). After clicking on , the window for addition of devices (see 6.1.1) to the system window appears. For every device to be controlled with «IC Net», open the group which contains this device, select the device and the Serial port of the interface where the device is connected to. After clicking on , the device is added to the SYSTEM window.
4 Systems After clicking on , the user is asked to save the new system as a system file (*.smt) in the new system folder. The new system is now ready to start a determination (see section 4.3.3). 4.1.2 System window A system window is opened with IC NET / File / Open / System and the selection of the desired system file. It contains icons for Data recorder, Watch window (screen) and all the devices installed with the system wizard. Watch window Data recorder 4.1.
4.2 System file handling 4.2 System file handling The following menu items are used for opening, changing, saving and closing of systems: IC NET / File / Open / System Load an existing system file (*.smt) from the Systems directory and open the corresponding SYSTEM window. The name of the folder and of the system file are displayed in the title bar of the SYSTEM window. A star ( * ) at the end of the name indicates that the system settings have been changed since the last saving.
4 Systems SYSTEM / Control / Disconnect from workplace Disconnect selected system from the workplace. If a system is disconnected, manual control is not available for this system, but all other system settings can be modified and saved. Start determination and Startup hardware is only possible if no other system from the same system folder is connected. In this case, the system is automatically connected. 4.3.
4.3 System functions 4.3.3 Start/stop determinations SYSTEM / Control / Start determination Start determination using the settings of the selected system. At this start command, the System startup values are set at the devices. The time programs of the different devices and the data recording are started either immediately (Start with determination) or after switching the injection valve to the "Inject" position (Start with inject) as set in the Start mode window.
4 Systems Ident Calibration level Info 1 / Info 2 Volume Dilution Vial number Amount User defined identifier (title) for the chromatogram to be displayed in the title bar of the chromatogram window and in the Chromatogram open window. Calibration level (0 = sample; 1…n = calibration solutions). Sample description. Injected volume in µL. Dilution of the sample. Autosampler vial position to take sample from. Sample amount.
4.4 System settings 4.4 System settings 4.4.1 Modify system window SYSTEM / Setup / Drag icons If this option is enabled, the device icons can be resized and moved in the SYSTEM window and the SYSTEM window itself can be resized. To resize an icon or the window, move the cursor to the desired appears. Press the left mouse button and resize object until the object to the desired size. To move an icon, move the cursor to the desired object until appears.
4 Systems The time program of the loaded object or the data acquisition (RECORDER) is started at the moment the sample is injected. Start with inject The objects can be moved from one field to the other using the or 4.4.4 buttons. Install new devices SYSTEM / Setup / New devices/ Install new device Add new devices to the open SYSTEM window.
4.5 System state window 4.4.7 Show system parameters SYSTEM / Setup / Parameters / Startup hardware Display of the name of the method linked to the system, the main measurement channel, the configuration settings for the devices, and the system startup values. SYSTEM / Setup / Parameters / Start with determination Display of the objects defined in the Start mode window. The time programs are displayed if the programs are enabled.
4 Systems 4.5.2 Running Running program or chromatogram data acquisition. Running program (xxx min left) Running time program with time display. Waiting for INJECT Waiting for "INJECT" to start program and/or chromatogram data acquisition as defined in the Start mode. INJECT done Injection valve has been switched to the "INJECT" position. Stopping Determination has been stopped. Finished Program or chromatogram data acquisition has been finished. SHUTDOWN System is shutdown.
4.5 System state window IC Net 2.2 E295 Storage of memory values failed (repeat last action; if error reappears, call Metrohm service). E296 Instrument stopped (restart instrument; if error reappears, call Metrohm service). E297 Storage of remote line values failed (repeat last action; if error reappears, call Metrohm service). E298 Storage of flow correction value failed (repeat last action; if error reappears, call Metrohm service).
5 Interfaces 5 Interfaces The «IC Net» program supports remote control for interfaces available from Metrohm and Bischoff. Interfaces generally convert analog signals to digital form, which can be handled by the computer. Interfaces are stored as device files (*.dev) in the Devices directory. All Metrohm interfaces and Metrohm devices start with Me*.dev; all Bischoff interfaces and Bischoff devices start with Bi*.dev. 5.1 Interface installation 5.1.
5.1 Interface installation Details to the other steps of the system wizard procedure (installation of devices, processing method, and data source) see section 4.1.1. 5.1.2 Add interfaces in workplace window IC NET / Options / Devices setup Open the WORKPLACE window which shows all interfaces and devices installed. This window can be opened only by the user with Administrator access level.
5 Interfaces 5.2 762 IC Interface 5.2.1 762 IC Interface features The Metrohm 762 IC Interface is available as a one system version (2.762.0010) or a two system version (2.762.0020). Each system can control a complete ion chromatography or HPLC equipment and contains the following parts: • Two 24-bit Analog-to-Digital Converters (often abbreviated as ADC) to acquire two analog signals from two different detectors simultaneously (for example conductivity detection and UV detection).
5.2 762 IC Interface 5.2.4 762 IC Interface window 762 icon / Open The 762 IC Interface window for parameter settings is opened by selecting this menu option with the right mouse button or by clicking the 762 icon on the toolbar. It consists of three tabs Init, RS settings, and Links. Init The Init tab of the 762 IC Interface window contains data acquisition parameters for the 762 IC Interface. Label Optional label to name the interface with maximum 8 characters.
5 Interfaces Read current parameters from 762 IC Interface. Send current parameters to 762 IC Interface. RS settings The RS settings tab of the 762 IC Interface window contains RS232 parameters for the 762 IC Interface. Baud Rate Parity Handshake FIFO size Baud rate of the device. Selection: 1200...115200 Default value: 115200 Parity check. Selection: None, Even, Odd Default value: None Enable/disable software handshake mode.
5.2 762 IC Interface The Links tab of the 762 IC Interface is used for COM port selection and settings. Alias Name of the interface or device. COM # If this entry is clicked with the right mouse button, the following menu appears: Possibility for setting COM port parameters (details see on-line help). Possibility for changing the COM port. The following window is opened, where the COM port can be changed by clicking on the desired entry. Put on desktop Change 5.2.
5 Interfaces the determination is started (Start with determination) or at the moment the sample is injected (Start with inject). The Program tab contains the two following subpages: Program Main time program with all program steps. Events configuration Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. First column Time at which program instruction is applied. Entry range: 0.0 ...
5.2 762 IC Interface List of program instructions The following program instructions can be added to the time program on the Program subpage: Instruction Parameter entry Meaning Events 0, 1, p, * Set event output lines 1...7 to the desired values. For entry of the first value, enter 1, 0, p or *. For entry of the other values, move the cursor in front of the value to be changed and enter 1, 0, p or *.
5 Interfaces Event command (2nd column) Setting the event output lines 1…7. Selection: 0 (line off, inactive, open) 1 (line on, active, 0 V) p (pulse, pulse length 150 ms) * (leave line in current status) For entry of the first value, enter 1, 0, p or *. For entry of the other values, move the cursor in front of the value to be changed and enter 1, 0, p or *. Add new remote command. Delete selected remote command.
5.3 771 Compact Interface 5.3 771 Compact Interface 5.3.1 771 Compact Interface features The Metrohm 771 Compact Interface is a two channel Analogto-Digital Converter to acquire two analog signals from two different detectors simultaneously or time independent. It contains the following parts: • Two 24-bit Analog-to-Digital Converters (often abbreviated as ADC) to acquire two analog signals from two different detectors simultaneously (for example conductivity detection and UV detection).
5 Interfaces Range Range for AD converter. Example: converts ± 2500 mV to 224 bits Entry range: 2500, 1250, 625, 312.5, 156.25, 78.125, 39.062 mV Read current parameters from 771 Compact Interface. Send current parameters to 771 Compact Interface. RS settings The RS settings tab of the 771 Compact Interface window contains RS232 parameters for the 771 Compact Interface. Baudrate Parity Handshake 46 Baud rate of the device. Selection: 1200...115200 Default value: 57600 Parity check.
5.4 Metrohm PC Board Read current parameters from 771 Compact Interface. Send current parameters to 771 Compact Interface. Links The Links tab of the 771 IC Interface is used for COM port selection and settings (details see section 5.2.4 Links). 5.4 Metrohm PC Board The 2.714.0310 Metrohm PC Board Interface is a two channel Analog-to-Digital Converter (often abbreviated as ADC) to acquire two analog signals from two different detectors simultaneously or time independent (e.g.
5 Interfaces 5.5 Global timer 5.5.1 Timer icon The global timer is used for programming system tasks which are started automatically daily or once at the desired time. The Global timer icon is available on the toolbar if the Timer has been installed with the new system wizard or by using the option of the Workplace window (see section 5.1.2). By clicking this icon the Timer window for parameter settings opens. 5.5.
5.5 Global timer Add a new subtask for the selected daily task. The time and program instruction for this subtask has to be entered in the opened subwindow and the system file to which this instruction should be applied must be selected in the Systems folder. Delete the selected task from the timer program. The following program instructions can be used for userdefined event program tasks in the Timer program window: Open Close Startup Start Stop Shutdown IC Net 2.
6 Devices 6 Devices The «IC Net» program supports remote control for Metrohm and Bischoff instruments. Each instrument within a system is a device. Devices are stored as device files (*.dev) in the Devices directory. All Metrohm devices start with Me*.dev and all Bischoff devices start with Bi*.dev. 6.1 Device installation 6.1.1 Install devices at system creation IC NET / File / New / System The New system wizard window and a new SYSTEM window are opened.
6.1 Device installation For every device to be controlled with «IC Net», open the group which contains this device, select the device and the Serial port of the interface where the device is connected to. After clicking on , the device is added to the SYSTEM window. If desired, an additional data recorder and a timer of the More modules group can be added to the system. Details to the other steps of the system wizard procedure (processing method, data source) see section 4.1.1. 6.1.
6 Devices 6.2 Watch window 6.2.1 Watch window icon The watch window icon is one of the components always present in a new SYSTEM window. If the system is connected and this icon is clicked with the right mouse button, the following menu item appears: 6.2.2 Open Open the WATCH WINDOW for live display of instrument values (this window can also be opened by double-clicking the watch window icon). Unlink Delete the WATCH WINDOW from the SYSTEM window (not recommended).
6.2 Watch window The colors of the watch window fields and of the SYSTEM STATE window can be changed by clicking the fields with the right mouse button. This offers the following possibilities: Choose color / Foreground / Out of range Set display color for field numbers if the measurement value is out of range (overflow or out of limits). Choose color / Foreground / In range Set display color for field numbers if the measurement value is inside the normal range.
6 Devices 6.3 732 IC Detector 6.3.1 732 IC Detector features The Metrohm 732 IC Detector is a conductivity detector for ion chromatography. The associated thermostatable detector block can be used for recording of chromatograms with and without chemical suppression and is normally installed in the 733 IC Separation Center. The two following versions are available: • 2.732.0010 IC Detector with standard detector block • 2.732.
6.3 732 IC Detector 6.3.3 732 IC Detector window 732 icon / Open The 732 IC Detector window for parameter settings is opened by selecting this menu option with the right mouse button or by double-clicking the 732 icon in the SYSTEM window. It consists of the four tabs Display, Method parameter, Program, and Links. Display The Display tab of the 732 IC Detector window is only available for a connected system. It displays the current measurement values and parameters settings of the 732 IC Detector.
6 Devices Range Full scale Cell const. Thermostat Offset Damping Display of measuring range in µS/cm. Display of full scale range in µS/cm. Display of cell constant of the conductivity cell in /cm. Display of temperature within in the detector block. Display of offset of the zero point. Display of electronic damping of the analog output. Zero Triggers the auto-zero function. Switch off the auto-zero function. Update all display values.
6.3 732 IC Detector Range Measuring range in µS/cm Selection: 100, 200, 500 µS/cm and 1, 2, 5, 10 mS/cm Full scale The full scale range (operating range) sets the desired sensitivity for the analog output. The possible values of the full scale range in µS/cm depend on the preset measuring Range. Ion Combination of polarity and temperature coefficient: Anion polarity = +, temperature coeff. = 2.5 Cation polarity = –, temperature coeff. = 1.
6 Devices The Program tab contains the two following subpages: Program Remote configuration Main time program with all program steps. Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. First column Second column Third column ENABLED Will be executed by 58 Time at which program instruction is applied. Entry range: 0.0 ... 999.
6.3 732 IC Detector List of program instructions The following program instructions can be added to the time program on the Program subpage: Instruction Parameter entry Meaning Remote 0, 1, * Set remote output lines 1...8 to the desired values. For entry of the first value, enter 1, 0 or *. For entry of the other values, move the cursor in front of the value to be changed and enter 1, 0 or *. Flag reset, end Set program flag.
6 Devices Pmax 0.0 ... 25.0 MPa Set maximum pressure limit for the high-pressure pump connected to the 732 IC Detector to the desired value. Pmin 0.0 ... 25.0 MPa Set minimum pressure limit for the high-pressure pump connected to the 732 IC Detector to the desired value. Remote configuration On the Remote configuration subtab user-defined remote commands can be defined, which can be inserted into a time program after being activated with .
6.4 761 Compact IC 6.4 761 Compact IC 6.4.1 761 Compact IC features The Metrohm 761 Compact IC is a PC-controlled system for ion chromatographic analyses. The two following versions are available: • 2.761.0010 Compact IC without suppressor module • 2.761.0020 Compact IC with suppressor module All the parts of the 761 Compact IC like injection valve, highpressure pump, conductivity detector, suppressor module and peristaltic pump, are normally fully controlled by the «761 Compact IC» program.
6 Devices If the system is connected and the 761 icon is clicked with the right mouse button, the following menu appears: Open Open the 761 Compact IC window for parameter settings (this window can also be opened by double-clicking the icon). 6.4.3 Hardware Open the hardware settings window. Diagnostics Open the diagnostics window (for service personal only). Unlink Delete the 761 icon from the system.
6.4 761 Compact IC Conductivity, µS/cm Live display of measured conductivity. Pressure, MPa Live display of measured pressure. The color settings for this two fields can be changed by clicking the fields with the right mouse button (see section 6.2.2). Display of actual values. Flow, mL/min Display of current flow rate of the highpressure pump. Pressure max, MPa Display of current maximum pressure limit for high-pressure pump.
6 Devices Full scale, µS/cm Remote lines even without connection to the PC. Entry range: 0.0 ... 25.0 MPa Startup value for full scale range. Selection: 50, 250, 1000 µS/cm Startup value for remote line settings 1…8. Selection: 0, 1 Initial The Initial tab of the 761 Compact IC window for settings of system startup values is only available for a disconnected system. Flow, mL/min Pressure max, MPa Pressure min, MPa Full scale, µS/cm Remote lines Startup value for flow rate of the highpressure pump.
6.4 761 Compact IC The Program tab contains the two following subpages: Program Main time program with all program steps. Remote configuration Possibility for creation of user-defined remote commands. Program On the Program subpage, program steps including time, program instruction and parameter can be entered. IC Net 2.2 First column Time at which program instruction is applied. Entry range: 0.0 ... 999.
6 Devices List of program instructions The following program instructions can be added to the time program on the Program subpage: Instruction Parameter entry Meaning Valve Inject, Fill Switch injection valve to "INJECT" or "FILL" position. FullScale 50, 250, 1000 µS/cm Set full scale range to the selected value. ICPump on, off Switch on or off the high-pressure pump. Flow 0.2 ... 2.5 mL/min Set flow rate of the high-pressure pump to the desired value. Pmax 0.0 ... 25.
6.4 761 Compact IC Remote configuration On the Remote configuration subtab user-defined remote commands can be defined, which can be inserted into a time program after being activated with . Name (1st column) User-definable name of the remote command (e.g. Start_766). Remote command (2nd column) Setting the remote output lines 1…8. 0 (line off, inactive, open) 1 (line on, active, 0 V) p (pulse) * (leave line in current status) For entry of the first value, enter 1, 0, p or *.
6 Devices 761 unit version Selection of instrument version: 761.0010 = 761 Compact IC without suppressor 761.0020 = 761 Compact IC with suppressor Suppressor Suppressor module: Automatic switching to the next position each time the injection valve is switched either to the Fill or to the Inject position. Autostep with Use peristaltic pump If this option is disabled, the peristaltic pump is not switched on automatically at the start of a determination or with Startup hardware.
6.4 761 Compact IC 6.4.4 Hardware settings Selection of the Hardware menu item of the 761 icon using the right mouse button opens the hardware settings window consisting of the three tabs Hardware, Outputs and Alarm stops. Hardware The Hardware tab of the hardware settings window defines general parameters which are set automatically at power on of the 761 Compact IC. Remote lines after power on The remote output lines 1…8 are set to this values after power on or a manual stop with Shutdown hardware.
6 Devices Cell constant Cell constant of the conductivity cell for correct display of the absolute conductivity. Enter the cell constant printed on the detector block into this field. Range: 0.1 ... 99.9 /cm For a precise determination of the cell constant, pump a calibration solution of known conductivity through the IC system, observe the displayed conductivity and change the cell constant until the correct conductivity value is displayed.
6.4 761 Compact IC Pump stop (P max/min) Pump stopped because pressure limits are exceeded. Hardware error Hardware error detected at the 761 Compact IC (high-pressure pump, injection valve, or suppressor not working correctly). Alarm leak detector Leak detector has detected solvent in the instruments interior. Remote line Set remote output lines 1…8.
6 Devices Leak Power on 72 Leak detector has detected solvent in the instruments interior. This information is also stored in the instrument itself, so that it is stopped automatically even without connection to the PC. Temporary power failure at the 761 Compact IC hardware. IC Net 2.
6.5 817 Bioscan 6.5 817 Bioscan 6.5.1 817 Bioscan features The Metrohm 817 Bioscan is a pulsed amperometric detector for use with a modular IC system. The instrument is designed to carry out determination of carbohydrates but is also capable of analyzing alcohols (polyalcohols), sugar alcohols, as well as other oxidizable substances. The 817 Bioscan is fully operated using the «IC Net» program. It is normally connected directly to a PC COM port using the 6.2134.080 or 6.2134.090 cable and the 6.2125.
6 Devices Mode DC Pulse Scan Cell Oven Temperature Control Selection of detector mode: In DC mode, a constant potential is applied to the working electrode, the analytes are oxidized or reduced in accordance with their electrochemical properties. The Pulse mode works with three different working potentials, which are applied cyclically.
6.5 817 Bioscan Valve Analog output Actual Mode Cell Oven Control Manual switching of the injection valve at the 812 Valve Unit: Switch injection valve to "Fill" position (control line Cont1 is set to 0). Switch injection valve to "Inject" position (control line Cont1 is set to 1). Manual setting of the analog output signal to zero. Trigger the autozero function. Display of actual values. Display of current detector mode. Display of current cell state. Display of current ovent state.
6 Devices Oven Range Full scale Filter Offset Switching on/off column oven and setting operating temperature. Range: 15 ... 60 °C Selection of measuring range: pA 10 ... 5000 pA. nA 0.1 ... 50 nA. µA 0.01 ... 5 µA. The full scale range (operating range) sets the desired sensitivity for the analog output. The possible values of the full scale range depend on the preset measuring Range. Electronic damping of the analog output signal. Selection: 0.1, 0.2, 0.
6.5 817 Bioscan Sample time Oven Range Full scale Offset Measuring time at the end of working potential E1. Selection: 20, 40, 60, 80, 100 ms Switching on/off column oven and setting operating temperature. Range: 15 ... 60 °C Selection of measuring range: pA 10 ... 5000 pA. nA 0.1 ... 50 nA. µA 0.01 ... 5 µA. The full scale range (operating range) sets the desired sensitivity for the analog output. The possible values of the full scale range depend on the preset measuring Range.
6 Devices Oven Range Full scale Offset Cycle potentials Method Actual Display of current oven state. Display of current measuring range. Display of current full scale range. Display of current offset. Graphical display of cycle potentials. Display of working potentials set in the Method data frame. Display of current working potentials. Send current parameters immediately to the 817 Bioscan. Parameters are not stored in the system file (*.smt) as long as the file is not saved.
6.5 817 Bioscan Full scale Offset The full scale range (operating range) sets the desired sensitivity for the analog output. The possible values of the full scale range depend on the preset measuring Range. Offset of the zero point for the analog output signal. Selection: -50 ... +50 % (in steps of 10 %) Actual E1, E2 Scan cycle Scan rate Oven Range Full scale Offset IC Net 2.2 Display of current start and end potentials in V. Display of current scan cycle mode.
6 Devices Operations Caption State Possibility to enter a title which is written on top of the scan mode graphics window. The current date and time are added to the title defined in the Caption field. Display of current state. Start scan mode sweep. Stop scan mode sweep. Copy the content of the scan mode graphics window to the clipboard. Links The Links tab of the 817 Bioscan is used for COM port selection and settings (details see section 5.2.
6.6 733 IC Separation Center 6.6 733 IC Separation Center 6.6.1 733 IC Separation Center features The Metrohm 733 IC Separation Center is a thermally and electronically isolated wet part which accommodates injectors, columns, detectors, suppressor module and pulsation dampener and is controlled by and connected to the 732 IC Detector. The following versions are available: • 2.733.0010 IC Separation Center with 1 injector • 2.733.0120 IC Separation Center with 2 injectors, metal-free • 2.733.
6 Devices 6.6.2 733 IC Separation Center icon The 733 icon is available in the SYSTEM window if a 733 IC Separation Center has been installed with the New system wizard or by using the Setup/New devices/Install new device option of the SYSTEM window (see section 6.1.2). If the system is connected and the 733 icon is clicked with the right mouse button, the following menu appears: 6.6.
6.6 733 IC Separation Center 733 IC Separation Center connected to 732 733.0130 Separation Center connected to 762 Configuration Display of current instrument configuration. 733 IC Separation Center with 1 injector. 733 IC Separation Center with 2 injectors. valve A valve A + valve B valve A + suppressor 733 IC Separation Center with 1 injector and 1 suppressor module. Valve A fill/inject Display of current valve position.* Switch valve A to "fill" position.
6 Devices * only available for 733 IC Separation Center connected to 732 Time program The Program tab is only available for a 733.0XX0 Separation Center connected to a 762 IC Interface. The 733 IC Separation Center that is connected to a 732 IC Detector is controlled by the time program of the detector. On the Program tab of the 733.0XX0 Separation Center window a user-defined time program can be entered. This program is started automatically as defined in the Start mode window (see section 4.4.
6.7 709 IC Pump Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program page: Instruction Parameter entry Meaning ValveA fill, inject Switch injection valve A to “fill” or “inject” position. ValveB fill, inject Switch injection valve B to “fill” or “inject” position. Step Rotate suppressor to the next position.
6 Devices The cable connection between 709 IC Pump and 732 IC Detector, 762 IC Interface or PC must be made before switching on the instrument. In order to operate the 709 IC Pump with «IC Net», the external control must be switched on using the [ Ext. ] key. For detailed information about the 709 IC Pump see 709 Instructions for Use. 6.7.
6.7 709 IC Pump Pressure Flow IC Net 2.2 Live display of current pressure in MPa. This field is also available for the WATCH WINDOW. Live display of current flow in mL/min. This field is also available for the WATCH WINDOW. New flow A new flow can be set by moving the scroll bar, by entering a value or by changing the value using the up and down arrows. Entry range: 0.01 ... 5.00 mL/min Min/Max pressure The minimum and maximum pressure limit for the 709 IC Pump can be set in steps of 0.1 MPa.
6 Devices automatically switched off. At the same time, the Pressure field color in the 709 settings window and in the WATCH WINDOW is changed to the value set for Out of range. Send current parameters immediately to the 709 IC Pump (only available if pump is running). Parameters are not stored in the system file (*.smt) as long as the file is not saved. Set current parameters and start 709 IC Pump drive. Stop 709 IC Pump drive.
6.8 Metrohm solvent delivery unit 6.8 Metrohm solvent delivery unit 6.8.1 Metrohm SDU features A Metrohm SDU (Solvent Delivery Unit) system can be used for high-pressure gradient mixture of one to four eluents. For each eluent, a 709 IC Pump is used. Each 709 IC Pump must be connected to the 762 IC Interface using a 6.2134.080 or 6.2134.090 cable. Examples for interconnections with the 709 IC Pump can be found in the 762 Instructions for Use.
6 Devices The Manual tab of the Metrohm SDU window is only available for a connected system. Pressure Flow New flow A new flow can be set by moving the scroll bar, by entering a value or by changing the value using the up and down arrows. Entry range: 0.01 ... 20.00 mL/min (depending on number of pumps) Min/Max pressure The minimum and maximum pressure limit for the individual pump units can be set in steps of 0.1 MPa. Entry range: 0.0 ... 50.0 MPa.
6.8 Metrohm solvent delivery unit Stop after Time left Stop time for all pump drives. Entry range: 0 ... 99999 min Display of time left before pumps are stopped. Send current parameters immediately to the pump control system. Parameters are not stored in the system file (*.smt) as long as the file is not saved. Start all pump drives. Stop all pump drives. Program The Program tab of the SDU window contains a time program for the pump control system to define eluent gradients.
6 Devices Flow %B %C %D Overall flow of pump control system in mL/min. Entry range: 0.01 ... 20.00 mL/min (depending on number of pumps) Individual flow rate for pump B in percent of total flow. Entry range: 0 ... 100 % Individual flow rate for pump C in percent of total flow. Entry range: 0 ... 100 % Individual flow rate for pump D in percent of total flow. Entry range: 0 ... 100 % Enabled Enable time program start (a disabled program is not started).
6.8 Metrohm solvent delivery unit 709 IC Pump window for parameter settings and manual control of the selected pump. Pressure checking Selection of the pump whose pressure is checked using the Min/Max pressure limits. Time reaction for press. min/max overflow Time delay for pump stopping at Min/Max pressure limits. Links The Links tab of the Metrohm SDU is used for COM port selection and settings (details see section 5.2.4 Links). IC Net 2.
6 Devices 6.9 752 Pump Unit 6.9.1 752 Pump Unit features The Metrohm 752 Pump Unit is a 2-channel peristaltic pump which is normally used for operating the suppressor module in the 733 IC Separation Center by supplying regeneration and rinsing solutions. The 752 Pump Unit, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6.9 752 Pump Unit Manual The Manual tab of the 752 Pump Unit window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Start pump with startup hardware Automatic start of pump drive with Startup hardware. Time program On the Program tab of the 752 Pump Unit window a user-defined time program can be entered. This program is started automatically as defined in the Start mode window (see section 4.4.
6 Devices ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program page: Instruction Parameter entry Meaning Program RESET, END Set program flag.
6.10 753 Suppressor Module 6.10 6.10.1 753 Suppressor Module 753 Suppressor Module features The Metrohm 753 Suppressor Module consists of a control unit with a 2-channel peristaltic pump and a block containing a suppressor module. The 753 Suppressor Module, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6 Devices Manual The Manual tab of the 753 Suppressor Module window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Suppressor Rotate suppressor to the next position. The time since the last step action is displayed in the field beside the button. Start pump with startup hardware Automatic start of pump drive with Startup hardware.
6.10 753 Suppressor Module First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6 Devices 6.11 6.11.1 754 Dialysis Unit 754 Dialysis Unit features The Metrohm 754 Dialysis Unit is a unit for on-line sample dialysis before sample injection. It consists of a 2-channel peristaltic pump and a dialysis cell. The 754 Dialysis Unit, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6.11 754 Dialysis Unit Manual The Manual tab of the 754 Dialysis Unit window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Start pump with startup hardware Automatic start of pump drive with Startup hardware. Time program On the Program tab of the 754 Dialysis Unit window a user-defined time program can be entered. This program is started automatically as defined in the Start mode window (see section 4.4.
6 Devices Third column Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. Test the time program (error messages are displayed if program is wrong). List of program instructions The following program instructions can be added to the time program on the Program page: Instruction Parameter entry Meaning Program RESET, END Set program flag.
6.12 793 Sample Prep Module 6.12 6.12.1 793 Sample Prep Module 793 Sample Prep Module features The Metrohm 793 Sample Prep Module consists of a control unit with a 2-channel peristaltic pump and a block containing the sample preparation module. The 793 Sample Prep Module, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6 Devices Manual The Manual tab of the 793 Sample Prep Module window for manual operation is only available for a connected system. Pump Start pump drive. Stop pump drive. Actuator Rotate sample preparation module to the next position. The time since the last step action is displayed in the field beside the button. Start pump with startup hardware Automatic start of pump drive with Startup hardware.
6.12 793 Sample Prep Module First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6 Devices 6.13 6.13.1 812 Valve Unit 812 Valve Unit features The Metrohm 812 Valve Unit is a control unit with one or two built-in injection valves. The following versions are available: • 2.812.0010 IC Valve Unit with 1 injector • 2.812.0020 IC Valve Unit with 2 injectors The 812 Valve Unit, which is normally operated manually using the keypad of the instrument or automatically via 732 IC Detector via remote interface, can be fully operated using the «IC Net» program.
6.13 812 Valve Unit 6.13.3 812 Valve Unit window 812 icon / Open The 812 Valve Unit window for parameter settings is opened by se- lecting this menu option with the right mouse button or by doubleclicking the 812 icon in the SYSTEM window. It consists of the three tabs Manual, Program, and Links. Manual The Manual tab of the 812 Valve Unit window for manual operation is only available for a connected system. Valve A Switch valve A to "FILL" position. Switch valve A to "INJECT" position.
6 Devices First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). ENABLED Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6.14 816 IC Eluent Selector 6.14 6.14.1 816 IC Eluent Selector 816 IC Eluent Selector features The Metrohm 816 IC Eluent Selector is a step-gradient 3-port selector, which can select one of three solutions (eluents). The 816 IC Eluent Selector, which is normally operated manually using the keypad of the instrument, can be fully operated using the «IC Net» program. The 816 IC Eluent Selector is normally connected to the 762 IC Interface using the 6.2134.080 or 6.2134.090 cable and the 6.2125.150 Adapter.
6 Devices Manual The Manual tab of the 816 IC Eluent Selector window for manual operation is only available for a connected system. Current eluent Selection of Eluent A, Eluent B, or Eluent C. Switch valves to feed the selected eluent. Flow A Possibility to rename Eluent A. Possibility to rename Eluent B. Possibility to rename Eluent C. Aliases Flow B Flow C Time program On the Program tab of the 816 IC Eluent Selector window a userdefined time program can be entered.
6.14 816 IC Eluent Selector First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6 Devices 6.15 6.15.1 828 IC Dual Suppressor 828 IC Dual Suppressor features The 828 IC Dual Suppressor is a continuous, regeneration-free solid phase suppressor which is used to increase the sensitivity of the detection of anions in ion chromatography. The 828 IC Dual Suppressor, which is normally operated manually using the switches of the instrument, can be fully operated using the «IC Net» program. The 828 IC Dual Suppressor is normally connected to the 762 IC Interface using the 6.2128.180 cable.
6.15 828 IC Dual Suppressor Manual The Manual tab of the 828 IC Dual Suppressor window for manual operation is only available for a connected system. Suppressor Switch the Suppressor on. Switch the Suppressor off. Start with startup hardware Start the Suppressor with the startup of the system hardware. Time program On the Program tab of the 828 IC Dual Suppressor window a userdefined time program can be entered.
6 Devices First column Second column Third column Time at which program instruction is applied. Entry range: 0.0 ... 999.9 min If no time is entered, the program instruction is applied together with the last instruction with time entry. Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction.
6.15 828 IC Dual Suppressor Links The Links tab of the 828 IC Dual Suppressor is used for COM port selection and settings (details see section 5.2.4 Links). IC Net 2.
6 Devices 6.16 6.16.1 750 Autosampler 750 Autosampler features The Metrohm 750 Autosampler is a sampler for automatic sample injection for ion chromatography. It has a capacity of up to 128 samples each with an effective volume of ca. 700 µL. The 750 Autosampler, which is normally operated using the keypad of the instrument, can be fully operated using the «IC Net» program. The 750 Autosampler is normally connected to the 762 IC Interface using the 6.2134.
6.16 750 Autosampler 6.16.3 750 Autosampler window 750 icon / Open The 750 Autosampler window for parameter settings is opened by selecting this menu option with the right mouse button or by double-clicking the 750 icon in the SYSTEM window. It consists of the four tabs Manual, Injection, Diagnostics and Links. Manual The Manual tab of the 750 Autosampler window for manual operation is only available for a connected system.
6 Devices Loop size is Size of the sample loop at the 733 IC Separation Center. If the loop size is > 20 µL, only 3 injections per sample vial are possible. Move transfer needle to the desired vial position (1...128 or Home). Lower transfer needle to the desired position (1...4). Start manual rinsing using the desired vial (1...128). Switch injection valve at the 733 IC Separation Center to the desired position (FILL or INJECT).
6.16 750 Autosampler Manual injection Sample vial number
6 Devices 6.17 6.17.1 766 IC Sample Processor 766 IC Sample Processor features The Metrohm 766 IC Sample Processor is a sampler for automatic sample injection for ion chromatography. It has a capacity of up to 127 samples each with an effective volume of up to 11 mL. The 766 IC Sample Processor, which is normally operated using the keypad of the instrument, can be fully operated using the «IC Net» program. The 766 IC Sample Processor is normally connected to the 762 IC Interface using the 6.2134.080 or 6.
6.17 766 IC Sample Processor If the system is connected and the 766 icon is clicked with the right mouse button, the following menu appears: 6.17.3 Open Open the 766 IC Sample Processor window for parameter settings (this window can also be opened by double-clicking the icon). Timeout Open a window for setting the timeout time (in s) for the scan command. Unlink Delete the 766 icon from the system.
6 Devices Position Stop moving the rack to place the specified vessel at the needle. Selection of the vessel to be placed at the needle. Selection: sample, sample+1, spec.#, 1...n Lift Position Start raising or lowering the lift to the specified position. Stop raising or lowering the lift to the specified position. Selection of the position. Selection: shift, work, rinse, special, 0, 1...
6.17 766 IC Sample Processor Status display The current status of the 766 IC Sample Processor can be displayed on the Current state subtab of the Manual tab which is only available for a connected system. Sample Vial position Lift position Pump state Display of current sample position. Display of current beaker position. Display of current lift position in mm. Display of current pump state (on, off). Status of the 8 input lines Status of the remote input lines.
6 Devices Num Task Parameter Row number (read-only). Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. List of program instructions The following program instructions can be added to the time program on the Program tab: 124 Instruction Parameter entry Meaning Move sample, sample+1, spec.#, 1...
6.17 766 IC Sample Processor Ctrl "control command" Set the specified remote output signal. In addition to the normal control commands (details see 766 Instruction for Use) special commands defined on the Configuration tab can be used. Wait 1...9999 s Waiting time. StartDA Start data acquisition. Configuration The Configuration tab of the 766 IC Sample Processor window contains parameters settings on the five subtabs Rack, Vial positions, Needle positions, Control, and Scan.
6 Devices Vial positions Rack type Current rack type (read-only). Parameter Name for special vial position. Number of special vial position. Pattern Add a new special vial position. Delete the selected special vial position. Needle positions Rack type Current rack type (read-only). Parameter Name for special needle position. Lift position of special needle position in mm. Pattern 126 Add a new special needle position. Delete the selected special needle position.
6.17 766 IC Sample Processor Control Pattern Name for special control command. Remote line settings for special control command. Add a new special control command. Delete the selected special control command. Parameter IC Net 2.
6 Devices Scan Pattern Name for special scan command. Remote line settings for special scan command. Add a new special scan command. Delete the selected special scan command. Parameter Links The Links tab of the 766 IC Sample Processor is used for COM port selection and settings (details see section 5.2.4 Links). 128 IC Net 2.
6.18 788 IC Filtration Sample Processor 6.18 6.18.1 788 IC Filtration Sample Processor 788 IC Filtration Sample Processor features The Metrohm 788 IC Filtration Sample Processor is a sampler for automatic filtration and injection of samples. It has a capacity of up to 127 samples each with an effective volume of up to 11 mL. The 788 IC Filtration Sample Processor, which is normally operated using the keypad of the instrument, can be fully operated using the «IC Net» program.
6 Devices If the system is connected and the 788 icon is clicked with the right mouse button, the following menu appears: 6.18.3 Open Open the 788 IC Filtration Sample Processor window for parameter settings (this window can also be opened by double-clicking the icon). Timeout Open a window for setting the timeout time (in s) for the scan command. Unlink Delete the 788 icon from the system.
6.18 788 IC Filtration Sample Processor Position Stop moving the rack to place the specified vessel at the needle. Selection of the vessel to be placed at the needle. Selection: sample, sample+1, spec.#, 1...n Lift Position Start raising or lowering the lift to the specified position. Stop raising or lowering the lift to the specified position. Selection of the position. Selection: shift, work, rinse, special, 0, 1...
6 Devices Status display The current status of the 788 IC Filtration Sample Processor can be displayed on the Current state subtab of the Manual tab which is only available for a connected system. Sample Vial position Lift position Pump state Display of current sample position. Display of current beaker position. Display of current lift position in mm. Display of current pump state (on, off). Status of the 8 input lines Status of the remote input lines.
6.18 788 IC Filtration Sample Processor Num Task Parameter Row number (read-only). Program instruction (see below). Parameter for program instruction (see below). Enabled Enable program start (a disabled program is not started). Add new program instruction. Delete selected program instruction. List of program instructions The following program instructions can be added to the time program on the Program tab: IC Net 2.
6 Devices Ctrl "control command" Set the specified remote output signal. In addition to the normal control commands (details see 788 Instruction for Use) special commands defined on the Configuration tab can be used. Wait 1...9999 s Waiting time. StartDA Start data acquisition. Configuration The Configuration tab of the 788 IC Filtration Sample Processor window contains parameters settings on the five subtabs Rack, Vial positions, Needle positions, Control, and Scan.
6.18 788 IC Filtration Sample Processor Vial positions Rack type Current rack type (read-only). Parameter Name for special vial position. Number of special vial position. Pattern Add a new special vial position. Delete the selected special vial position. Needle positions Rack type Current rack type (read-only). Parameter Name for special needle position. Lift position of special needle position in mm. Pattern IC Net 2.2 Add a new special needle position.
6 Devices Control Pattern Name for special control command. Remote line settings for special control command. Add a new special control command. Delete the selected special control command. Parameter 136 IC Net 2.
6.18 788 IC Filtration Sample Processor Scan Pattern Name for special scan command. Remote line settings for special scan command. Add a new special scan command. Delete the selected special scan command. Parameter Links The Links tab of the 788 IC Filtration Sample Processor is used for COM port selection and settings (details see section 5.2.4 Links). IC Net 2.
6 Devices 6.19 6.19.1 Data recorder Data recorder icon The Data recorder icon is one of the components always present in a new SYSTEM window. If the system is connected and this icon is clicked with the right mouse button, the following menu appears: Open Load the processing method linked to the system and open an empty chromatogram window (this window can also be opened by doubleclicking the icon). Setup Open data recorder setup window for selection of processing method and data source (see below).
6.19 Data recorder Processing method Name of the method file (*.mtw) linked to the system. Open processing method. Select the method file (*.mtw) to be linked to the system. Connected data source Data source for main measurement chan Connected nel. Open the Data source window for selection of the data source: Connected data source. Available 762 IC Interface # ch1 to ch4 Data source channels of the 762 IC Interface.
6 Devices 709 IC Pump # flow Flow channel of the 709 IC Pump. Pressure channel of the 709 IC Pump. # pressure Pump control system (SDU) Flow channel of the SDU. # flow Pressure channel of the SDU. Percentage of Eluent A channel of the SDU. Percentage of Eluent B channel of the SDU. Percentage of Eluent C channel of the SDU. Percentage of Eluent D channel of the SDU.
6.20 System timer 6.20 6.20.1 System timer Timer icon The system timer is used for programming system tasks which are started automatically daily or once at the desired time. The System timer icon is available in the SYSTEM window if a Timer has been installed with the New system wizard or by using the Setup/New devices/Install new device option of the SYSTEM window (see section 6.1.2). 6.20.2 Timer program The timer program of the system program is identical to the global timer program (see section 5.
7 Methods 7 Methods A method contains all information necessary for data acquisition, integration, peak evaluation and quantification. It can be considered as the chromatogram template, i.e. chromatogram without raw data. Methods are stored as method files (*.mtw) in the Methods directory. Each system is linked to a method. This method is called processing method and is opened automatically at the start of a new determination. 7.
7.2 Passport 7.2.1 General Tab General of the passport with general description of the method and determination. Ident Duration METHOD DATA Date/time Last update Calibration level User Detector Run IC Net 2.2 User defined identifier (title) for the chromatogram to be displayed in the title bar of the chromatogram window and in the Chromatogram open window. Duration of the chromatogram in min. You can modify this value when the chromatogram is running.
7 Methods To reset the Run number press [Ctrl] [F8]. The Analysis number window is opened where the run number for the next run can be modified to the desired value. Batch 7.2.2 X/Y: total number of started sample queues / current analysis number in the sample queue (read-only). Sample Tab Sample of the passport with sample information. Info 1 Info 2 Volume Dilution Vial number Amount 144 First sample description (max. 256 characters). Second sample description (max. 256 characters).
7.2 Passport Internal standard amount Concentration of the internal standard component for relative concentration calculations. Date/time when... 7.2.3 Date and time of sample collection (the default values are equal to the date and time when the chromatogram starts). Column Tab Column of passport with column information. Number ID Length Serial number of column (max. 256 characters). Internal diameter of the column in mm. Length of the column in mm. This parameter is used to calculate Linear flow.
7 Methods 7.2.4 Eluent Tab Eluent of passport with eluent information. Eluent A Eluent B Eluent C Flow Pressure Temp. 146 Description of eluent composition (max. 256 characters). Description of eluent composition (max. 256 characters). Description of eluent composition (max. 256 characters). Flow rate of the high-pressure pump in mL/min or µL/min. The flow rate is used to recalculate the time axis into volumetric units.
7.2 Passport 7.2.5 Comment Tab Comment of passport for entry of free-text user comments into the chromatogram description. Use this feature to enter any additional information about the chromatogram not included in other sections of the method. IC Net 2.
7 Methods 7.3 Method setup IC NET / Method / Method setup This menu item opens the Method setup window which includes the most common parameters for data acquisition and evaluation of the method and consists of the following tabs: General Measure Filters Processing Math 7.3.1 General information on determination. Important data acquisition parameters. Parameters for noise filtration. Set actions that are performed when the chromatogram finishes.
7.3 Method setup 7.3.3 Filters Tab Filters of Method setup window with parameters for noise filtration of raw data. Three different filtration algorithms are available to reduce noise and increase apparent signal-to-noise ratio. Spikes Slit Median Slit Gaussian Raw data points Smallest peak has ... IC Net 2.2 Spikes filter. The Spikes filter smoothes the first and last points of the chromatogram and the points identified as spikes.
7 Methods 7.3.4 Processing Tab Processing of Method setup window for definition of actions that are performed during the run or when the chromatogram finishes. Automatic post-run data processing is especially useful for chromatographic systems that include an autosampler. Save chromatogram after the run If checked, autosaving of the chromatogram will be performed on data acquisition ending. while running every ...
7.3 Method setup Program before Program after 7.3.5 Name and path of the program to be started automatically before the run is started. Name and path of the program to be started automatically after the run is finished. Math Tab Math of Method setup window with parameters that are used for various types of calculations. Parameter Formula set Theoretical plates Resolution Asymmetry Formula Custom formulae IC Net 2.
7 Methods European Pharmacopoeia Formula setting according to European Pharmacopoeia. US Pharmacopoeia Formula setting according to US Pharmacopoeia. Calculation of void time/void volume: Calculation method Selection of the method for void time calculation: None Manual entry in the Void time field. 1st component The peak corresponding to the first component is selected as a void time marker and its retention time replaces the previous value of the void time.
7.4 Integration 7.4 Integration 7.4.1 General information IC NET / Method / Integration This menu item opens the Integration parameters window which contains parameters and events for peak integration and consists of the two tabs Setup (see section 7.4.2) and Events (see section 7.4.3). The «IC Net» software includes a built-in automatic integration algorithm to detect peaks on the chromatographic curve and to evaluate them using calculated baselines.
7 Methods 7.4.2 Setup Tab Setup of Integration parameters window with integration parameters. The two most important of these parameters for peak recognition are Width and Slope. If the signal-to-noise ratio of the chromatogram is high enough (e.g. 100:1 or higher), the integration algorithm is not too sensitive to changes in integration parameters. On the contrary, if the baseline noise is high, careful tuning of these parameters may be needed. Number of peaks Number of peaks detected (read-only).
7.4 Integration Broadening Slope Asymmetry Min area Min height Rider ratio Negative peaks width of the narrowest peak on the chromatogram (normally 2…10 s). Entry range: 0.1…480 s Broadening for peaks in the end of the chromatogram compared to peaks in the beginning. This parameter is used for automatic adjustment of Width. Entry range: 0.1…100 Threshold for peak recognition.
7 Methods 7.4.3 This function sets up reasonable integration parameters in the following way: • Slope is set to a reasonable value. • Asymmetry is set to a reasonable value. • Width and Broadening are calculated by fitting a straight line through the measured peak halfwidth values vs. retention time. Width is determined as the y value of this line at the Delay time, Broadening is calculated as the y value at the end of the chromatogram divided by the y value at the Delay time.
7.4 Integration If an integration event is added or modified, the Edit integration event window is opened where the following parameters can be set: Time Event Value Start time for integration event in min. List box with integration events to be selected (see below). Parameter value for selected integration event. This field appears for events that demand input of an additional parameter. Integration events for peak detection Set this mode to stop detection of new peaks.
7 Methods Integration events for peak start/end Force the beginning of a new peak. If a peak is available at the moment of the event it is either rejected (upslope) or terminated (downslope). Set peak end Force peak end at the time of the event. Upslope peaks are rejected (except those born by Set peak start event), downslope peaks are terminated. Stop single peak mode Disable peak end detection. All peaks of any group after this event will be treated as one peak. The group end will be treated as peak end.
7.4 Integration Set horizontal baseline Set horizontal baseline for all peaks except the last one of adjacent peaks that are not separated. The baseline is drawn forwards from the peak start point. Set back horizontal base Set normal baseline Set horizontal baseline for all peaks except the first one of adjacent peaks that are not separated. The baseline is drawn backwards from the peak end point. Set default baseline detection mode. Set baseline point at 4.5, 6, 7.2, 8.9 and 9.
7 Methods Force horizontal base back Cancel horizontal base back Force horizontal base back Set horizontal baseline for a single peak. The baseline is drawn backwards from the peak end point. The intersection of the baseline with the signal is defined as peak start point. Cancel horizontal base back Clear Force horizontal base back mode. Enable baseline penetration Disable baseline penetration Enable baseline penetration Allow crossing of the signal by the baseline.
7.5 Calibration and quantification 7.5 Calibration and quantification 7.5.1 General information The aim of any chromatographic analysis is to answer the question "What components are present in the sample and what are their concentrations?". Two procedures are used to achieve this goal: the first step is called calibration, the second step includes quantification.
7 Methods 7.5.2 Notations R Stands for response value, either area or height, depending on setting selected in the Calibration graphs window. V Sample Volume injected. D Dilution coefficient, shows number of times to which the initial solution is dissolved before injection. V' = V / D Adjusted volume of injected sample. A correction is made for the dilution coefficient. C Concentration of the component in the initial solution (before dilution).
7.5 Calibration and quantification 7.5.3 External standard calibration External standard calibration (absolute calibration) is a key way of calibration. Basically this procedure calculates the dependence of injected component's quantity versus area (or height) of the corresponding peak. This dependence is showed as a calibration plot with injected quantity along the Y axis and peak area (or height) along the X axis.
7 Methods 164 Number Row number (read-only). Peak Number of the peak in the chromatogram that corresponds to the given component. If this field contains a 0, the peak number must be entered manually. Time Expected retention time of the component in the calibration run. After a new entry of the peak number the corresponding retention time is entered automatically as soon as this field is clicked on with the mouse. Wind.% Identification window of the component.
7.5 Calibration and quantification min C (max C) Minimum (Maximum) concentration value for the component. Components whose concentration leaves the range min C…max C are marked in the peak table by the sign " ! ". Additionally, the component table contains the following buttons: Add a new component (an empty column) to the component table. Delete the current component from the component table. Open the Peak identification window (see section 7.5.5).
7 Methods 7.5.5 Peak identification IC NET / Method / Calibration / Identification Selecting this menu item or clicking on in the component table opens the Peak identification window with parameters for tuning the peak identification procedure. Number of components Number of components in the component table (read-only). Scheme Standard Nonstandard Identification Reference peaks Other peaks Identification parameters. Identification parameter for reference components.
7.5 Calibration and quantification Worst case... Information on the component with the worst (largest) deviation of actual and expected retention time. Deviation is given as a part of component’s identification window. Average relative deviation Relative deviation averaged for all components. 7.5.
7 Methods Level 1...Level N Contains concentrations (or other chosen values) of components for corresponding Calibration level. Add a new Calibration level to the concentration table. The following window appears: Create calibration level Number of calibration level to add. Fill level with concentration Concentration to be filled in for all components. Copy concentrations from level Number of calibration level from which concentrations are copied.
7.5 Calibration and quantification 7.5.7 Calibration curve IC NET / Method / Calibration / Graphs Selecting this menu item or clicking on in the component table opens the Component window. In this window the results of the calibration are shown for each component together with the calibration curve. The parameters for calculating the calibration curve can also be entered here.
7 Methods Calibration points table This table contains the basic information used to construct the calibration curve: Level Number of Calibration level. Conc. Concentration of the current component in the calibration sample. It is taken from the concentration table. Area (or Height) Peak height or area of the current component, depending on calibration base. File File name of calibration chromatogram that stores data on the given calibration level.
7.5 Calibration and quantification Base Base for calculations (Area or Height) in quantification and calibration procedures. Indicates, which parameter is to be used as a peak response. Reference channel Channel which is used to measure area or height (read-only). Formula Formula for calibration function. There are six possible calibration dependencies for linear and nonlinear calibration curves.
7 Methods amount of the injected components becomes equal to the declared value. This declared summary amount is entered into the Concentration field of the universal component for This run level. 7.5.9 Calibration data handling Load and save calibration data IC NET / Method / Calibration / Load from method Load the calibration from the method. This option is used to update the calibration for the current run by the one taken from the method used for acquisition.
7.6 Report output 7.6 Report output 7.6.1 Report options window IC NET / Method / Report options IC NET / Process / Make report The Report options window is divided into several regions that combine parameters for report output on their functionality and includes several different areas. The report output is started by clicking on . IC Net 2.
7 Methods 7.6.2 Items to report This part of the Report options window contains a list of important report items that can be included into the report on the user's choice: General Report date: Printed by: General description on the analysis from the Passport. The Report date and the logged-in user Printed by are also put out. 07/12/1999 15:59:10 Roland Dörig Ident: Tap water Analysis from: 10/11/1999 16:17:02 File: jb101617.chw 16/11/1999 15:02:14 Modified! Method: Multicalibration.
7.6 Report output Chromatogram plot Plot of the chromatogram (only for Printer or File destination). Peak table Peak table with results. The structure of the peak table depends on the selected Quantification method. The following report is put out as default: Quantitation method: Custom No Retention Height Area Conc. min mV mV*sec mg/L 1 3.04 1.85 21.062 0.079 2 5.91 82.44 1703.350 7.173 3 6.83 3.58 97.495 0.000 4 14.29 29.27 969.705 7.561 5 21.21 19.34 935.179 5.
7 Methods 7.6.3 More items to report This part of the Report options window contains a list of rarely used report items that can be included into the report on the user's choice: Parameters used for data acquisition from the from the General, Measure and Filters tabs of the Method setup window. Acquisition ACQUISITION PARAMETERS Channels: Method duration: Run duration: Measurements (method): Measurements (run): Freq.
7.6 Report output 4 5 6 7 11.19 14.60 16.80 21.26 5.0 5.0 5.0 5.0 9.933e-03 7.912e-03 1.556e-02 6.021e-03 Calibration results CALIBRATION OF COMPONENT Method: Equation: RSD: Correlation 0.00 7.56 0.00 5.57 0.000 0.000 0.000 0.000 0 0 0 0 S bromide nitrate phosphate sulphate Calibration results of the component window. For each component a new page with calibration results and calibration curve is put out. fluoride Multicalibration.mtw Q = - 7.48163·A^2 + 0.00262326·A + 0.0233088 0.
7 Methods Zero Maximum Range Coefficient Noise Shift (multi)Channel ratio 178 parameter is used to detect an underflow condition. A signal level on a baseline (in ADC conversion units). This parameter is a result of the ADC calibration. Maximum value of the linear range of the AD interface (in ADC conversion units). This parameter is used to detect overflow condition. An input signal value in maximum in userdefined units.
7.6 Report output 7.6.4 Report destination The following output devices can be selected simultaneously in any combination as targets for report output: Screen Printer File 7.6.5 Output report (without curves) to screen. Output report to printer. Output report to file (see section 7.6.7).
7 Methods As long as no universal component is defined, only peaks with non-zero concentrations are listed. The peak table includes the following columns: Peak number, Retention time, Height, Area, Response factor, Concentration, Concentration%, Name Relative concentration Index Column test Custom Standard component 180 This quantification method uses the internal standard method to calculate the relative concentrations of the components.
7.6 Report output Concentration of std Concentration of the internal standard component for relative concentration calculations. This value is stored in This run column of the concentration table. Total % for normalization A value to which the sum of concentrations is normalized. It is used for Response normalization and Normalized concentrations quantification methods. The default value is 100. Printing order By peaks By components IC Net 2.2 Determines the order of the components in the peak table.
7 Methods 7.6.6 Template options For report output using a report template the following parameters can be modified: 7.6.7 Template Selection of the report template file *.rtt. Several templates for different languages and applications are available. Separator Defines which character separates report columns in tables. A separator other than Space is useful when the report is printed to a file and this file is imported into a spreadsheet software. Tab size Sets tab stop value for the screen window.
7.6 Report output 7.6.8 Report elements If Quantification method = Custom has been selected, the peak table can be customized on the user's choice. After clicking the button, the following columns can be included in the peak table. number Peak number. retention time Retention time of the component (in minutes, irrespective of the chosen retention units on the chromatogram graph axes). The total value in the column is equal to the chromatogram duration.
7 Methods where i and i+1 indexes refer to the neighboring peaks, and w0.607 stands for the peak width at 60.7 % of the peak height. effectivity, TP Effectivity for the peak in number of theoretical plates. The number of theoretical plates Ni per column for a chosen peak is calculated for a chromatographic peak by one of two formulas: Ni = 2 PI (ti • Hi / Ai)2, where PI = 3.1415926..., ti = retention time, Hi = height, Ai = area of the peak. The more commonly used formula is: Ni = 5.
7.6 Report output asymmetry PGF = 1 for an ideal peak with gaussian shape. Peak asymmetry As is calculated at 1/10 of the peak height as a ratio of width after the top of the peak w2 to the width before the top w1. As = w2 / w1 response factor Coefficient k1 of the calibration curve. raw concentration Absolute concentrations (raw quantity) of the components, calculated as Ci = Wi(Ri) / V' Total value for the column is the sum of concentrations for all components.
7 Methods peak amount Absolute amount of substance which is contained in the sample loop volume. It is the product of the absolute concentration C(i) multiplied to the volume of the sample loop. The amount is displayed without unit in the report, pay attention to the order of magnitude of the displayed value! index Linear or logarithmic retention index for identified components. Total value for the column is a weighted index average with absolute concentration as weight. I = ∑ { (Ii Ci) / ∑ Ci }.
7.6 Report output The complete component type may look like: BBD : !R. IC Net 2.2 group Number of the group for the component. spectral ratio Ratio of responses on different channels for multi-channel chromatograms. name Name of the component. file name File name of chromatogram. This column is very useful for the purpose of processing of exported data. ident Title (identifier) of the chromatogram. This column is useful for the processing of exported data.
8 Chromatograms 8 Chromatograms 8.1 Chromatogram window The CHROMATOGRAM window is used to show a running or recorded chromatogram. Title bar Status bar Chromatogram The title bar of the chromatogram window contains buttons for minimizing, maximizing and closing the window. The window name consists of the elements "Ident (method name) chromatogram name". A star ( * ) at the end of the name indicates that the chromatogram has been changed since the last saving. The status bar contains two fields.
8.2 Chromatogram file handling During active data acquisition the elapsed time, the duration of the chromatogram or program and the current X and Y values are shown in the second field of the status bar. If the peak editor is switched on then the position of the cursor will be shown. A chromatogram can be scaled with the help of keyboard or mouse functions or through the Chromatogram axes window opened with IC NET / View / Appearance / Chromatogram axes.
8 Chromatograms Filename Selected Directory Filename (common wildcards as * and ? are possible). Number of selected chromatograms and their total size. Path name of the working directory File window Box with the list of files residing in the working directory sorted by time (the last recorded chromatogram is at the top of the list). As additional short information on the chromatogram the two parameters Ident and Method are also displayed.
8.2 Chromatogram file handling 8.2.2 Save chromatogram IC NET / File / Save / Chromatogram Save the selected chromatogram in a chromatogram file (*.chw) in the working directory. If this chromatogram has been already stored in this directory, the message File ... exists. Overwrite? appears. If it is preferable to overwrite the previous copy (for example, after the reprocessing), press the button. Pressing saves the chromatogram into the new file (a new copy of the chromatogram is created). 8.2.
8 Chromatograms Represent raw data as Output of raw data: Default units (float) Y values are exported in default units for the channel. ADC response (integer) Y values are exported in bits. Include retention time In seconds (float) As point number 8.2.6 X values are exported in seconds. X values are exported as point number. Import chromatogram IC NET / File / Import / Chromatogram Import chromatogram of the following formats: *.chm Chromatogram files of the «Chrom&Spec» program (DOS version). *.
8.3 Graphical representation 8.3 Graphical representation 8.3.1 Appearance IC NET / View / Appearance This menu item opens the Appearance window. It determines the appearance of the chromatogram and consists of four tabs: Chromatogram axes Labels Select channel Colors Scaling of chromatogram axes. Settings for peak labels and baseline drawing. Select channels to be displayed (only available for multi-channel chromatograms). Color settings for chromatogram elements.
8 Chromatograms Response axis Y from Y to Tic marks Beginning of the window on Y axis. End of the window on Y axis. Plot axes labels and tic marks on Y axis with no, relative or absolute scaling. View all Set scales on X and Y axes so that the whole chromatogram is visible. Drift compensation Turns the chromatogram screen image so that the last and first points of the chromatogram are on the same level. This option is useful for gradient chromatograms.
8.3 Graphical representation Component name Name of the component. Component name + Quantity Name and quantity of the component. Channel labels At start In corner Label for data acquisition channel. Channel label is shown at the start of the chromatogram. Channel label is shown in the upper right corner of the chromatogram. Show baseline and peaks Baselines are shown. Baseline marker Start and end of baselines are marked. Label always visible Label is shown always with zooming.
8 Chromatograms Colors Tab Colors of the Appearance window with color settings parameters for chromatograms. this color is default Hair Background Axes Baseline AlterGround Channel 1...8 Line width Line width for the selected element (Axes or Channel 1...8) in % of selected plot font height. Range: 0 ... 232 Set all Set colors of all opened chromatograms automatically to the settings in the selected chromatogram window.
8.3 Graphical representation 8.3.2 Other graphical functions IC NET / View / X full scale By selecting this menu item or pressing [ Ctrl ] + [ Home ] the X axis is scaled so that the chromatogram perfectly fits the window horizontally. IC NET / View / Y full scale By selecting this menu item or pressing [ Ctrl ] + [ End ] the Y axis is scaled so that the chromatogram perfectly fits the window vertically.
8 Chromatograms 8.4 Peak editor 8.4.1 Switching on/off the peak editor IC NET / Process / Peak editor The peak editor mode is used for subsequent manual correction of the automatic integration for chromatogram peaks (see section 7.4). It can be used to select the most important points for the peak evaluation (start, end and top of peak, valley between peaks) and move them to the required position. If the peak editor mode is enabled, the vertical bar cursor appears in the chromatogram window.
8.4 Peak editor IC NET / Peak / Select end point Move the cursor to the end of the nearest peak and select the peak. IC NET / Peak / Select valley point Move the cursor to the valley of the two nearest peaks. IC NET / Peak / Unselect peak Delete the selection. [–] Move a selected point (start, top, end, valley) to the cursor position. IC NET / Peak / Move selected point [+] Merge two neighboring peaks into a single peak.
8 Chromatograms 8.5 Printing 8.5.1 Page layout for printing IC NET / File / Page layout The selection of this menu item or clicking on in the Report options window opens the Page layout window for page layout parameters. Length units inches centimeters Page margins Left Right Top Bottom Selection of length units. Inches. Centimeters. Selection of page margins. Left margin. Right margin. Top margin. Bottom margin.
8.5 Printing 8.5.2 Save set page layout parameters as default values. Printer settings IC NET / File / Printer setup By selecting this menu item the Print setup window is opened where printer, paper size and format can be defined. 8.5.3 Print preview IC NET / File / Preview By selecting this menu item the Preview window appears on the screen where the report is shown in the appearance formatted for the desired printer as defined in the Report options window. 8.5.
8 Chromatograms 8.6 Miscellaneous functions 8.6.1 Reintegration IC NET / Process / Reintegrate This menu item opens the Integration parameters window where the reintegration of the chromatogram can be started (details see section 7.4). 8.6.2 Recalibration IC NET / Process / Calibrate This menu item opens the Recalibration window for entry of the calibration level and recalibration of the chromatogram using this level (details see section 7.5). 8.6.
8.6 Miscellaneous functions Meaning Meaning of the signature, can be selected from a pop-up list that is predefined in the Meaning set window. Actions Sign the chromatogram. Revoke the signature. Once a chromatogram is signed it cannot be altered. To alter a signed chromatogram the signature has to be revoked. Close the Electronic signature window . Opens the Meaning set window.
8 Chromatograms IC NET / Process / Electronic signature This window displays the signatures of the chromatogram in the active window. The signature table can be sorted according to one of the five columns by clicking the title. A star ( * ) at the end of the title indicates the sorting criterion. 8.6.
8.6 Miscellaneous functions 8.6.5 Compare chromatogram IC NET / Process / More / Compare This menu item opens the Compare with window that allows the comparison of the active open chromatogram to any other opened chromatogram or method. The chromatogram (or method) that is to be compared has to be the active window. Compare the selected file of the list to the chromatogram (or method) of the active window and display the result in the Differences window.
8 Chromatograms File Close Close window and return to Compare with window. Edit Copy Left frame Right frame 8.6.6 Copy the data of the comparison to the clipboard. Overview of the found differences, arranged in a menu tree. Click on the interesting menu point to display its properties in the Right frame. The displayed menu point is inverted. Table of the property values of the selected menu point.
8.6 Miscellaneous functions 8.6.7 Invert chromatogram IC NET / Process / More / Invert! This menu item inverts the response curves for all channels of the chromatogram so that negative peaks become positive and vice versa (useful for chromatograms with wrong input polarity). 8.6.8 Autodatabase IC NET / Method / Autodatabase The Autodatabase options window contains parameters and settings for sending chromatograms to the Autodatabase program. Database file Definition of the database file (*.
8 Chromatograms Close the window and abandon changes. For details on the «Autodatabase 1.0» program, open this program and call on-line help. IC NET / Options / Indicate AutoDB server Indicate Autodatabase program icon on the task bar if it has been already activated in the background by sending data. 208 IC Net 2.
9.1 Sample queue file handling 9 Sample queue A sample queue is a table containing sample-specific data which is used to facilitate work with autosamplers and multiple analyses. The number of rows of the sample queue table defines the number of determinations which are run automatically. Once the queue has been started, the sample-specific data are transferred line-by-line to the running determination overwriting the corresponding fields of the method. A sample queue is stored in a sample queue file *.
9 Sample queue 9.2 Sample queue control 9.2.1 Sample queue overview table IC NET / File / Open / Sample queue SYSTEM / System / Sample queue These two menu items open the OPEN window for opening an existing sample queue file *.que or creation of a new sample queue file *.que by entering a new name. After confirmation with the sample queue overview table is opened showing the current status of the sample queue: No System Ident Vial Inj-s Started Level Volume 210 Row number.
9.2 Sample queue control Amount Sample amount. If this value is different for the calibration run (c) and the sample run (s), the component concentrations of the sample are calculated as follows: Cs = Cc • Amounts / Amountc Shut down system after the queue finishes If this option is enabled, the system hardware is shut down after the sample queue has been finished (all pumps are switched off), but it remains connected.
9 Sample queue 9.2.4 Cancel last run SAMPLE QUEUE OVERVIEW / Control / Cancel last run Cancel the last run (set Started = 0 for this row). If the queue is restarted, this row is executed again. This function is only available if the sample queue has been interrupted by pressing the button. 9.2.5 Reset sample queue SAMPLE QUEUE OVERVIEW / Control / Reset Reset sample queue table (set Started = 0 for all rows).
9.3 Sample queue editor Vial Volume Dilution Amount Autosampler vial position to take sample from. Injected volume of sample in µL. Sample dilution prior to injection. Sample amount. If this value is different for the calibration run (c) and the sample run (s), the component concentrations of the sample are calculated as follows: Cs = Cc • Amounts / Amountc Internal standard amount Level Injections Done Sample Info 1 Sample Info 2 9.3.
9 Sample queue QUEUE EDITOR / Edit / Paste row(s) Paste the rows from the clipboard into the sample queue table. QUEUE EDITOR / Edit / Delete row(s) Delete the selected rows from the sample queue table. QUEUE EDITOR / Edit / Duplicate row(s) Duplicate the selected rows of the sample queue table. QUEUE EDITOR / Edit / Increment Fill selected column fields with values automatically incremented by 1. The last character of the first selected field must be a number.
10.1 Batch reprocessing queue file handling 10 Batch reprocessing Reprocessing is understood to be the subsequent reprocessing of a series of chromatograms which have been loaded in a queue (Batch reprocessing queue). For reprocessing according to a selected method the settings for calibration, integration, passport, appearance and report can be altered at will. A batch reprocessing queue is stored in a batch reprocessing queue file *.bar in the Data directory. 10.
10 Batch reprocessing 10.1.3 Save batch reprocessing queue QUEUE EDITOR / File / Save Save the batch reprocessing queue in a batch reprocessing queue file (*.bar) in the working directory. The batch reprocessing queue editor window remains open. QUEUE EDITOR / File / Save & exit Save the batch reprocessing queue in a batch reprocessing queue file (*.bar) in the working directory and close the batch reprocessing editor window. 10.2 Perform batch reprocessing 10.2.
10.2 Perform batch reprocessing Reprocess calibration runs Reprocess all calibration chromatograms (calibration level > 0). Update method file in directory after reprocessing Save the method file *.mtw after reprocess- ing if the method is changed. Reintegrate Reintegrate chromatograms according to the current settings of the integration parameters and integration events.
10 Batch reprocessing Default scheme switched on) by updating the concentration table. Default setting for recalibration reprocessing. The two options Apply final calibration... and Forget calibration points... are switched on. A new calibration is performed with the calibration runs and the resulting new calibration parameters (component table, concentration table and calibration curve) are applied to all sample runs.
10.2 Perform batch reprocessing ters Ident, Sample Info 1 and Sample Info 2 entered in the batch reprocessing table are overwritten if these values are modified in the Passport window. Modify chromatogram appearance If this option is enabled, the chromatogram Appearance parameters which have been changed after clicking the button are applied to all chromatograms. Open the Appearance window for modification of the settings for chromatogram axes, labels and colors.
10 Batch reprocessing Statistics Options REPROCESS / The Statistics options window is used to set the parameters for the statistical evaluation of the loaded batch reprocessing file. Property Select the property which is to be evaluated statistically. It can be selected by clicking the pop-up menu.
10.2 Perform batch reprocessing Open the Advanced window for export settings of the statistics. Advanced REPROCESS / / The Advanced window is used to specify Path and File name of the output file. If a Viewer program for the output file is specified, the file opens with this viewer when the file is created. This feature can be used for further processing of the statistical data.
10 Batch reprocessing The statistics output file is a ASCII file. It can be edited by any text editor, but the file structure of the output file allows also the processing of this file in MS Excel. This provides access to all the powerful spread sheet functionalities of this program and thus an easy opportunity to create meaningful diagrams from your statistical data. The statistics output file is a ASCII file, even if it is saved with the *.xls file extension.
10.3 Batch reprocessing queue editor The chromatogram axes, labels and colors can be set in the Appearance window. The multi-channel chromatogram can be saved with File / Save / Chromatogram. 10.3 Batch reprocessing queue editor 10.3.1 Open batch reprocessing queue editor window REPROCESS / This menu item opens the batch reprocessing editor program for editing the batch reprocessing table. No File name Method Ident Vial Volume Dilution Amount Row number.
10 Batch reprocessing Calibration level Sample Info 1 Sample Info 2 10.3.2 Calibration level (see section 7.5) for the sample: Level 0 stays for normal analysis, levels 1 and greater for calibration runs. Correct filling of this column enables to calculate calibration coefficients automatically. First sample description. Will be placed into appropriate passport field. Second sample description. Will be placed into appropriate passport field.
10.3 Batch reprocessing queue editor Save the modified batch reprocessing queue and close the QUEUE EDITOR window. QUEUE EDITOR / File / Exit Close the batch reprocessing QUEUE EDITOR window without saving the modified batch reprocessing table. IC Net 2.
11 Appendix 11 Appendix 11.1 Software license The use of the Software is subject to this License Agreement between you and Metrohm AG. With the offer, you have received this License Agreement and taken note of it. You have already accepted this License Agreement upon the placement of your order with Metrohm AG or one of its distributors or upon confirmation of the order by Metrohm or one of its distributors.
11.1 Software license 5. Metrohm and its distributor disclaim all other warranties and liability. In particular, Metrohm and its distributor assume no liability for third-party damage or consequential damage, loss of data, lost profits, interruption of business, etc. 6. This Agreement applies until it is terminated. You can terminate this Agreement by destroying the Software and every copy of it.
11 Appendix 11.2 Declaration of conformity – Software validation The software "Metrodata IC Net 2.2" was developed in accordance with the requirements of the ISO 9001 quality system regarding the design, testing and servicing of Metrodata software. The relevant procedures are described in the document "Project procedure for creating Metrodata software" which is available at your Metrohm agency on request.
11.3 Index 11.3 % %B %C %D Index ...................................................................90,92 ...................................................................90,92 ...................................................................90,92 1 1st component ......................................................152 1st peak................................................................152 2 21 CFR Part 11.....................................................23 21 CFR Part 11 On ...........
11 Appendix 788 IC Filtration Sample Processor configuration................................................. 134 features......................................................... 129 icon ............................................................... 129 links............................................................... 137 manual control.............................................. 130 program ........................................................ 132 program instructions ...........................
11.3 Index Autodatabase options .....................................207,219 Automatic logout after ..............................................19 Available..........................................................21,139 Average relative deviation.......................................167 Axes .....................................................................196 Axes labels .........................................................194 B Background ......................................................
11 Appendix data compression ........................................ 206 definition ........................................................... 8 delete ............................................................ 191 directory for saving....................................... 150 duration......................................................... 143 export............................................................ 191 file handling .................................................. 189 identifier ........
11.3 Index D ..................................................................48 Damping ............................................................56,57 Danger.................................................................... 3 DATA....................................................................143 Data acquisition 762 parameters...............................................39 771 parameters...............................................45 channel .........................................
11 Appendix Enable detection ................................................... 157 Enable negative peaks ........................................... 157 Enable peak reject ................................................. 157 Enable valley-to-valley ........................................... 158 Enabled...................................... 92,111,114,124,133 ENABLED .................42,58,65,84,96,99,102,105,108 END................................................................. 43,66 Error messages ..
11.3 Index I IC Applications Collection (8.732.2013) ................ 2 IC pump..................................................................63 Icons 709 IC Pump ...................................................86 732 IC Detector...............................................54 733 IC Separation Center ...............................82 750 Autosampler...........................................116 752 Pump Unit ................................................94 753 suppressor Module...................
11 Appendix List of program instructions......43,59,66,85,96,99, ............................102,105,108,111,114,124,133 .................................................... 200 ................................................... 196 Local.................................................................... 170 Lock system ........................................................... 19 Logarithmic index .................................................. 145 Login ...............
11.3 Index Number of peaks ...................................................154 Number of theoretical plates.......................151,184 O Offset .................................................56,57,76,77,79 OK, Apply buttons mean ...........................................23 .................................................................48 On-line ...................................................................33 On-line help ..........................................................
11 Appendix Printing range..................................................... 201 Processing .................................................... 150,188 Processing method............................. 9,25,139,142 Program 732 time program........................................... 57 733 time program........................................... 84 752 time program........................................... 95 753 time program........................................... 98 754 time program....................
11.3 Index Respnsibility .........................................................203 Response ...........................................................162 Response axis .......................................................194 response factor .....................................................185 Response factor .................................................164 Response normalization .........................................179 Result report .......................................................
11 Appendix Software validation............................................. 228 Sort window............................................................ 21 Sort Sequence ........................................................ 21 Spikes.................................................................. 149 Split peak ............................................................. 158 Standard component ...................................... 171,180 ...........................................
11.3 Index ............................................................166 Upload buffer ........................................................138 UploadStartupValues ...............................................33 US Pharmacopoeia ................................................152 Use method from file for reprocessing .....................216 Use peristaltic pump ................................................68 Used ....................................................................170 User .......
