Owner's manual

7 Methods
IC Net 2.0
120
7.5.2 Notations
R Stands for response value, either area or height,
depending on setting selected in the Calibration
graphs window.
V Sample Volume injected.
D Dilution coefficient, shows number of times to
which the initial solution is dissolved before in-
jection.
V' = V / D Adjusted volume of injected sample. A correction
is made for the dilution coefficient.
C Concentration of the component in the initial so-
lution (before dilution).
Q = C • V' Quantity of component, used for calibration curve
construction.
t Retention time. Time needed by the mobile phase
to flow through the separation system.
t
0
Void time. Dead time needed by the mobile
phase to flow through the separation system.
t' = t – t
0
Corrected retention time, called also net retention
time.
L Column length.
v = L / t
0
Linear Flow rate.
W(R) = k
2
R
2
+ k
1
R + k
0
Calibration function (component quantity W vs.
detector response R). In the case of the most
common linear calibration curve Q = W(R) = k
1
R
it comes through the origin. The concentration of
the component in the analyzed mixture is calcu-
lated by the formula C = W(R) / V'.
RSD(Q, R) Procedure, used for computation of regression
coefficients (k
0
, k
1
and k
2
) of the calibration
function W(R) using RSD (Residual Standard
Deviation). The procedure gets input as a set of
calibration points (quantity Q vs. response R)
and outputs the calibration function W(R) used
for prediction of the component quantity Q
i
=
W(R
i
).
Subscript values used:
j Stands for j-th calibration level run.
s Stands for standard component.
i Stands for component number.