871 Advanced Bioscan CH-9101 Herisau/Switzerland E-Mail info@metrohm.com Internet www.metrohm.com Instructions for Use 8.871.
CH-9101 Herisau/Switzerland E-Mail info@metrohm.com Internet www.metrohm.com 871 Advanced Bioscan 8.871.1003 Instructions for Use 8.871.1003 01.
Teachware Metrohm AG Oberdorfstrasse 68 CH-9101 Herisau teachware@metrohm.com 1. Edition 2007 These instructions are protected by copyright. All rights reserved. Although all the information given in these instructions has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please inform the author at the address given above.
1.1 Instrument description Table of contents 1 Introduction ................................................................ 1 1.1 1.2 Instrument description ................................................................................1 Parts and controls .......................................................................................2 1.2.1 1.2.2 1.2.3 1.3 Information on the Instructions for Use .....................................................6 1.3.1 1.3.2 1.4 Front....................
1 Introduction 4.2.4 4.3 4.3.1 4.3.2 4.3.3 4.3.4 4.4 4.5 4.6 Connections ..................................................................................................... 24 Maintenance and servicing .......................................................................25 General information.......................................................................................... 25 Shutdown .........................................................................................................
1.1 Instrument description List of figures Figure 1: Figure 2: Figure 3: Figure 4: Figure 5: Figure 6: Front 871 Advanced Bioscan .................................................................... 2 Rear 871 Advanced Bioscan ..................................................................... 3 Interior 871 Advanced Bioscan.................................................................. 4 Mains connection plug ............................................................................
1.1 Instrument description 1 Introduction 1.1 Instrument description The 2.871.0010 Bioscan is a PC-controlled measuring instrument for the sensitive analysis of carbohydrates by ion chromatography using pulsed amperometric detection.
1 Introduction 1.2 Parts and controls In this Section you will find the numbers and designations of the parts and controls of the 871 Advanced Bioscan. The numbering applies throughout the Instructions for Use, i.e. bold numbers in the text (e.g. 4 ) refer to the parts and controls illustrated here. 1.2.
1.2 Parts and controls 1.2.2 Rear 5 12 6 7 8 9 Figure 2: 5 Remote connection A 6 Mains switch to switch instrument on and off: I = ON 0 = OFF 7 Fuse holder changing the fuses, see section 2.2.1 8 Mains connection plug mains connection, see section 2.2 871 Advanced Bioscan / Instructions for Use 8.871.1003 10 11 Rear 871 Advanced Bioscan 9 Connection C Manual valve (not used with IC Net) 10 Remote connection B 11 RS232 interface Connection of the PC; see chapter 5.
1 Introduction 1.2.3 Interior 34 33 32 30 31 13 14 15 16 17 18 Metrosep Carb 1 19 20 21 22 23 24 25 26 Figure 3: 28 29 Interior 871 Advanced Bioscan 13 Column connection capillary Connection to Flow cell PEEK capillary 4 27 14 Flow cell see section 2.4.7 871 Advanced Bioscan / Instructions for Use 8.871.
1.2 Parts and controls 15 Electrode cable (black) connection for reference electrode 16 IC column e.g. Metrosep Carb 1 (6.1013.000) for carbohydrate analysis 17 Column holder 25 Connection capillary for eluent. connection 818 IC Pump – Pulsation absorber 26 PEEK capillary 26 Pulsation absorber 6.2620.150 18 Injection valve see section 2.4.
1 Introduction 1.3 Information on the Instructions for Use Please read through these Instructions for Use carefully before you put the 871 Advanced Bioscan into operation. The Instructions for Use contain information and warnings to which the user must pay attention in order to assure safe operation of the instrument. 1.3.1 Organization These Instructions for Use 8.871.
1.3 Information on the Instructions for Use 1.3.2 Notation and pictograms The following notations and pictograms (symbols) are used in these Instructions for Use: Fill SYSTEM STATE 23 Menu item, parameter or entry value in the software Program window in the software Button in the software Part or control of 871 Hazard This symbol draws attention to a possible danger to life or of injury if the associated directions are not followed correctly.
1 Introduction 1.4 Safety notes 1.4.1 Electrical safety While electrical safety in the handling of the 871 Advanced Bioscan is assured in the context of the specifications EN 61010-1 (protection class I, degree of protection IP20), the following points should be noted: • Mains connection Setting of the mains voltage, checking the mains fuse and the mains connection must be effected in accordance with the instructions in Section 2.2.
2.1 Setting up the instrument 2 Installation 2.1 Setting up the instrument 2.1.1 Packaging The 871 Advanced Bioscan is supplied together with the separately packed accessories in special packaging containing shock-absorbing foam linings designed to provide excellent protection. The actual instruments are packed in an evacuated polyethylene bag to prevent the ingress of dust. Please store all this special packaging as only it can assure transport of the instruments free from damage. 2.1.
2 Installation 2.2.1 Fuses Fuses 2.5AT/250V are installed in fuse holder 7 of the 871 Advanced Bioscan. Ensure that the instrument is never put into operation with fuses of a different type as this could cause a fire! For checking or changing fuses, proceed as follows: 1 Disconnect mains cable Disconnect mains cable from mains connection plug 8. 2 Remove fuse holder Take fuse holder 7 until it dangles (on the bottom of the fuse holder is a small opening to grip it).
2.3 Connection to PC Any break in the earthing inside or outside the instrument can make it a hazard! Mains connection Plug the mains cable into mains connection plug 8 of the 871 Advanced Bioscan (see Figure 2). 2.2.3 On/off switching of the instruments The 871 Advanced Bioscan is switched on and off using mains switch 6. Do not switch the IC components on before all cable connections have been established. 2.3 Connection to PC 2.3.
2 Installation Reports Systems Folder for storage of report files (*.txt) and graphic files (*.wmf) Folder with subfolders with system files (*.smt) and sample queue files (*.que). The installed files (incl. system and method files) are generally not write-protected. To prevent these files from being deleted by mistake, switch on the write-protection or make a backup copy in another directory. 2.3.
2.4 Capillary connections 2.4.2 Connect Drainage tube Connect silicone tube 6.1816.050 to the drain opening 3 and lead it to a waste container. 2.4.3 Connecting the pulsation absorber The 818 IC Pump needs to be deaerated before putting it into operation. The procedure is described in the 818 IC Pump Instructions for Use. To protect the column material from any pressure shocks which the injection may cause we recommend the insertion of the 6.2620.
2 Installation 2 2.4.5 Connect outflow tube • Lead PEEK capillary 29 through opening 2 of the Advanced Bioscan and connect it to the injection valve 18. Lead the other end to a waste container. Installation of the precolumn The use of easily exchangeable precolumns protects the separating columns and prolongs their lifetime. Recommended precolumn for the 871 Advanced Bioscan: Metrosep Carb 1 Guard.
2.4 Capillary connections 2.4.7 2 Rinse the IC column • Place a beaker beneath PEEK capillary 13. • Switch on the 818 IC Pump for 10 min. 3 Fix IC column • Fix the IC column 16 in column holder 17. Measuring cell Install the measuring cell according to the measuring cell documentation. 871 Advanced Bioscan / Instructions for Use 8.871.
3 Basic principles 3 Basic principles 3.1 Introduction The 871 Advanced Bioscan can be operated as an amperometric detector in three different working modes: 3.2 • DC mode A constant potential is applied to the working electrode. The analyte substances are oxidized or reduced according to their electrochemical properties. The current that is produced is measured. • Scan mode Current-potential curves are recorded in order to determine the optimum parameters for amperometric detection (DC and Pulse).
3.3 Pulsed amperometric detection a displacement of the characteristic current/potential curves (voltammograms). Possible results are the reduction of the signal intensity and lower signal/noise ratios. In order to ensure that a stable baseline and reproducible measuring conditions are obtained care should be taken that the pH of the eluent is correct. • Pulsation 3.3 Electrochemical reactions at the electrode surfaces depend on the transport of the reacting substances to the electrode.
3 Basic principles 3.3.1 Optimization of the PAD parameters When adapting the method parameters the preset parameters should initially be used. Descriptions of various applications are available from Metrohm AG in the form of Application Works and Application Notes. These can be obtained from your local Metrohm agency or on the Internet under www.metrohm.com. The potential profile shown in Figure 5 must always be matched to the analyte under investigation.
3.4 Optimization of the measuring potential 3.4 Optimization of the measuring potential An optimization of the measuring potential for amperometric detection may bring benefits in the following situations: a) The sensitivity of the detection of the analyte is to be increased against the background signal. b) The selectivity of the detection is negatively affected by the analyte peak overlapping with a second substance peak that has not been optimally separated by chromatography.
3 Basic principles worry about contamination of your IC system you can still inject the substance into the eluent using a large sample loop (> 500 µL). The eluent should then be pumped at a very low flow rate (e.g. 0.2 mL/min). Generally, same conditions should be used as they are needed for the chromatographic separation (e.g. temperature, pH, eluent etc.).
3.4 Optimization of the measuring potential Hydrodynamic voltammogram Scan voltammogram Situation • Selectivity for an inadequate IC-separation is to be improved • Sensitivity of a substance peak is to be increased Requirement • Substance can be investigated in the mixture • Substance must be present in a pure form • IC parameters must be known Advantages • "Chromatographic" conditions, i.e.
4 Notes - Maintenance - Faults 4 Notes - Maintenance - Faults 4.1 Handling the 871 Advanced Bioscan General Amperometric detection takes place with a flowing current and therefore with a chemical conversion of the analyte. The course of a chemical reaction depends directly on various physical parameters, among other things. In order to obtain optimum measuring conditions (e.g. stable baseline or reproducible signals) it is necessary to take the following points into consideration.
4.2 Practical notes on ion chromatography fices for the analysis problems at hand. Compare the standard chromatogram enclosed with the column with your own measurements. If any difficulties arise, you should always first check the quality of the column by recording a standard chromatogram.
4 Notes - Maintenance - Faults Contaminated valves are cleaned by rinsing with water or organic solvents. When the cleaned valves are reinstalled, you must ensure that the flow direction is correct. The replacement of piston seals has to be done in accordance with the pump manufacturer’s directions. The corresponding maintenance work for the 818 IC Pump is described in the 818 Instructions for Use. Salt crystals between the piston and the seal are the cause of abrasive particles, which can enter the eluent.
4.3 Maintenance and servicing 4.3 Maintenance and servicing 4.3.1 General information Care The 871 Advanced Bioscan requires proper care and attention. Excessive contamination of the instruments could possibly lead to malfunctions and a shorter service life of the mechanical and electronic parts. Spilled chemicals and solvents should be wiped up immediately. It is especially important to protect the plug connections at the rear of the instrument (particular the mains plug) against contamination.
4 Notes - Maintenance - Faults 4.3.3 Cleaning the working electrode The surface of the gold working electrode should be cleaned whenever you notice that its electrochemical properties have changed. This may be caused by the deposition of reaction products. High current flows can also alter the electrode surface; this is indicated by a very reduced sensitivity after longer periods of use. In principle, you should avoid too high analyte concentrations or dirty samples.
4.3 Maintenance and servicing Also change the precolumn when you change the separation column. 1 Remove old column • Switch off flow cell. • Switch off pump drive of the 818 IC Pump. • Unscrew connection capillary 21 to flow cell from the column. 2 Connect new column to injector • Remove end caps from column. • Screw preheating capillary 31 to inlet end of separating column (note flow direction). 3 Rinse column • Place beaker beneath the column outlet.
4 Notes - Maintenance - Faults 4.4 4.4.1 Faults and malfunctions Malfunctions and their rectification If difficulties arise with the IC system during analyses, their causes are best investigated in the order separating column → pump → eluent → 871 Advanced Bioscan. Several of the malfunctions which may appear are listed in the following table with details of possible causes and countermeasures.
4.4 Faults and malfunctions • Wrong IC-Net parameter 'Range' • Check settings • Damaged working electrode • Replace working electrode • Flow cell is not connected properly • Check cable connections (REF: black, WE: red, AUX: blue) • Wrong measuring potential • Optimize measuring potential Considerable pressure drop • Leak in system • Check connections and make leakproof Considerable pressure rise • Contamination of the filter in the 6.2821.100 Filter unit PEEK • Replace the 6.2821.
4 Notes - Maintenance - Faults 4.5 Instrument test with the dummy cell If any interference occurs whose source is suspected to lie in signal recording or transfer you can selectively check the instrument electronics and the connection to the PC. This is done by connecting the 6.2813.030 Dummy Cell supplied. The temperature in the 871 Advanced Bioscan should be kept constant (30 °C) and, for optimal shielding, the front door should be closed. This dummy cell contains a resistor (300 MΩ) and a condenser (0.
4.6 Diagnostic tests / Validation / GLP 4.6 Diagnostic tests / Validation / GLP The requirements of GLP (Good Laboratory Practice) include a periodic check of analytical measuring instruments with regard to their reproducibility and accuracy using Standard Operating Procedures, SOP. Under the title «Application Bulletin No. 277 – Validation of Metrohm ion chromatographs» an example of such a standard operating procedure is available from Metrohm; it can be adapted and used with the 871 Advanced Bioscan.
5 Appendix 5 Appendix 5.1 Technical data Reference conditions:: Environment temperature Oven temperature Relative humidity Instrument condition Adjustment interval 5.1.1 5.1.2 Measuring unit Operating modes DC, Pulse and Scan Potential Range: Resolution: -2.00 ...+ 2.00 V 10 mV Operating modes DC mode Pulse mode Scan 32 +22 °C (± 3 °C) +35 °C 50 - 65 % >45 min in service yearly Range: Resolution: Filter (avg) Resolution filter Noise 10 pA – 200 µA 1, 2, 5 Steps off, 0.2, 0.
5.1 Technical data 5.1.3 5.1.4 5.1.5 Autozero Function Compensation of actual cell current. Maximum compensation Range: 10 pA - 1 nA: 2 nA - 5 nA: 10 nA - 50 nA: 100 nA - 2 µA: 5 µA - 200 µA: Initiation Externally (RS232), I/O Injection valve Positions Fill .... Inject Material Peek Port connections 10-32 male threaded fittings Max. pressure 41 MPa Oven Temperature range Accuracy Stability 5.1.6 5.1.7 5.1.8 Compensation: 25 nA 250 nA 2.5 µA 25 µA 2.5 mA 8 °C above ambient to 45 °C 0.
5 Appendix Output lines 5.1.9 Signal output Analog output signal 5.1.10 5.1.11 5.1.12 5.1.13 34 Connection A, sensor board Relais: 'RELAY 1' and 'RELAY 2' TTL Input: 'CELL ON', 'RESET', 'CELL OFF', 'START' and 'AUTOZERO' TTL Output: 'OVERLOAD', 'AUX1', und 'AUX2' Range -10 V ... +10 V or -1 V ... +1 V Resolution 20-Bit DA converter Offset -50 % ... +50 % from max. output voltage Resolution 5 % steps Safety specifications Construction / testing According to EN 61010-1 , UL 61010A-1, CSAC22.
5.1 Technical data Width 260 mm Height 381 mm Depth 355 mm Weight 11 kg (without accessories) 871 Advanced Bioscan / Instructions for Use 8.871.
5 Appendix 5.2 Standard equipment Subject to changes ! All dimensions are given in mm. 2.871.0010 Advanced Bioscan Quantity Order no. Description 1 1.871.0010 871 Advanced Bioscan 1 6.1816.060 Drain tubing to UV/VIS Compact IC and Bioscan Drainage tubing for draining off escaped liquid Material Silicone 1 6.1825.
5.2 Standard equipment Quantity Order no. 1 6.2156.000 Description Cable to Bioscan flow cell Cable Bioscan - measuring cell 1 6.2156.010 Recorder cable for Bioscan Analog cable Bioscan - recorder, for converted signal (not required in IC system). 1 6.2620.150 Pulsation absorber (metal-free) For all ion chromatographs Material PEEK (metalfree) in contact with the eluent 2 6.2744.010 Height (mm) 41.5 Outer diameter in mm 76 Pressure screw 5x With UNF 10/32 connection.
5 Appendix Quantity Order no. 1 6.2802.100 Description Polishing plate for Bioscan flow cell Polishing plate for Bioscan working electrode 1 6.2802.110 Diamond paste for Bioscan flow cell Polishing paste for Bioscan electrode 1 6.2813.030 Test cell for Bioscan Test cell for checking electronic functions of Bioscan. 1 6.6034.033 IC Net 2.3 CD Software for data handling (storing, printing, displaying curves) and methods, complete remote instrument use. Dialog language: English CD.
5.3 Optional accessories 5.3 Optional accessories Order no. Description 6.1254.010 Gold flow cell for Bioscan Flow-through cell for Bioscan, electrode material: gold Material Gold Working electrode 6.1254.020 Distance piece for Bioscan flow cell, 50 µm 50 µm Spacer for Bioscan flow-through cell Material Plastic Height (mm) 0.05 6.1254.050 Glassy carbon flow cell for Bioscan Flow cell for Bioscan, electrode material: glassy carbon Material Glassy carbon Working electrode 6.1254.
5 Appendix Order no. Description 6.1254.070 Silver flow cell for Bioscan Flow cell for Bioscan, electrode material: Silver Material Silver Working electrode 6.1254.110 Au Variocell to Advanced Bioscan Gold measuring cell with replaceable electrode disks. 6.1254.120 Glassy Carbon Variocell to Advanced Bioscan Glassy carbon measuring cell with replaceable electrode disks. 6.1254.130 Platinum Variocell to Advanced Bioscan Platinum measuring cell with replaceable electrode disks. 6.1254.
5.3 Optional accessories Order no. Description 6.1803.030 PTFE capillary 0.5 mm i.d. / 3 m Capillary for inline dialysis, for Dialysis Unit, IC Dialysis Sample Processor, IC Liquid Handling Dialysis Unit Material PTFE Outer diameter in inches Inner diameter in mm 1/16 0.5 6.2156.030 Remote cable for Advanced Bioscan Remote cable Advanced Bioscan - IC Sample Processor 6.2156.040 Cable to Variocell Electrode Cable Advanced Bioscan - Variocell 6.2156.
5 Appendix Order no. Description 6.5331.010 Equipment set for Au flow cell of Bioscan Measuring cell for Bioscan with gold working electrode 6.5331.020 Equipment set for GC flow cell of Bioscan Measuring cell for Bioscan with glassy carbon working electrode 6.5331.030 Equipment set for Pt flow cell of Bioscan Measuring cell for Bioscan with platinum working electrode 6.5331.040 Equipment set for Ag flow cell of Bioscan Measuring cell for Bioscan with silver working electrode 6.5331.
5.3 Optional accessories Order no. Description 6.9988.713 Validation Folder for 871 (English) 871 Advanced Bioscan / Instructions for Use 8.871.
5 Appendix 5.4 5.4.1 Warranty and Conformity Warranty The warranty on our products is limited to defects that are traceable to material, construction or manufacturing error which occur within 12 months from the day of delivery. In this case, the defects will be rectified in our workshops free of charge. Transport costs are to be paid by the customer. For day and night operation, the warranty is limited to 6 months. Glass breakage in the case of electrodes or other parts is not covered by the warranty.
5.4 Warranty and Conformity 5.4.2 Declaration of Conformity This is to certify the conformity to the standard specifications for electrical appliances and accessories, as well as to the standard specifications for security and to system validation issued by the manufacturing company. CH-9101 Tel. Fax Herisau, Switzerland +41 71 353 85 85 +41 71 353 89 01 www.metrohm.com Name of commodity 871 Advanced Bioscan Name of manufacturer Metrohm Ltd.
5 Appendix 5.4.3 Quality Management Principles Metrohm Ltd., CH-9101 Herisau, Switzerland CH-9101 Herisau/Switzerland E-Mail info@metrohm.com Internet www.metrohm.com Metrohm Ltd. holds the ISO 9001 Certificate, registration number 10872-02, issued by SQS (Swiss Association for Quality and Management Systems). Internal and external audits are carried out periodically to assure that the standards defined by Metrohm’s QM Manual are maintained.
5.5 Index 5.5 Index 7 709 IC Pump Maintenance.............................. 23 8 871 Advanced Bioscan Handling .................................... 22 A Abrasive particles .......................... 24 ambient temperature..................... 22 Amperometric detection................ 16 Analog signal 12 Figure........................................... 3 Appendix ................................... 6, 32 Application Notes .......................... 23 Application Works .........................
5 Appendix Figure .........................................10 Mains connection plug 8 Figure .....................................3, 10 Mains switch 6 Figure .....................................3, 10 On/off switching of the instruments ................................11 Maintenance ............................22, 25 Malfunctions...................................28 Manual valve 9 Figure ...........................................3 Measuring cell holder 34 Figure ...........................................
