CH-9101 Herisau/Switzerland E-Mail info@metrohm.com www.metrohm.com 838 Advanced Sample Processor Program version 5.838.0012 Installation Instructions 8.838.1313 09.
Teachware Metrohm AG Oberdorfstr. 68 CH-9100 Herisau teachware@metrohm.com These instructions are protected by copyright. All rights reserved. Although all the information given in these instructions has been checked with great care, errors cannot be entirely excluded. Should you notice any mistakes please inform the author at the address given above.
Table of contents 1 Introduction 1 1.1 Instrument description ......................................................................1 1.1.1 Scope of application of the 838 Advanced Sample Processor ................1 1.1.2 Instrument models and available versions ................................................2 1.2 Information about this Installation Instructions ..............................4 1.2.1 Note.....................................................................................................
3.3.15 Connecting pumps ..................................................................................35 3.3.16 Connector dosing drives and stirrers ......................................................35 3.3.17 Installing the catch pan ...........................................................................36 3.3.18 Sample racks ...........................................................................................36 3.3.19 Fitting the safety guard/splash guard .................................
6.1.2 Filter working life ......................................................................................68 6.1.3 Filter membrane selection .......................................................................68 6.2 Technical data ................................................................................ 70 6.2.1 2 Channel peristaltic pump......................................................................70 6.2.2 Pump connections ............................................................
List of illustrations Figure 1 Models 2.838.0x10 without injection valve ..................................................................... 2 Figure 2 Models 2.838.0x20 with injection valve........................................................................... 2 Figure 3 Overall view..................................................................................................................... 5 Figure 4 Rear panel..................................................................................
1.1 Instrument description 1 Introduction This Chapter offers you an initial overview of the Metrohm 838 Advanced Sample Processor and its instrument versions. It tells you how you can use this versatile instrument and familiarises you with the most important operating controls. 1.1 Instrument description 1.1.
1.1.2 Instrument models and available versions There are various models and available versions of the Metrohm 838 Advanced Sample Processor with various accessories. Instrument models • 838 Advanced Sample Processor with Swing Head, peristaltic pump and 2 pump connections • 838 Advanced Sample Processor with Swing Head, peristaltic pump, injection valve, and 2 pump connections Figure 1 Models 2.838.0x10 without injection valve Figure 2 Models 2.838.
1.1 Instrument description Available versions 2.838.0010 Advanced IC Sample Processor Model without injection valve, without keypad, with standard accessories 2.838.0020 Advanced IC Injection Sample Processor Model with injection valve, without keypad, with standard accessories 2.838.0110 Advanced IC Dialysis Sample Processor Model without injection valve, without keypad, with dialysis accessories 2.838.
1.2 Information about this Installation Instructions 1.2.1 Note Attention! Please study these Installation Instructions carefully before you start to use the Instrument. The instructions contain information and warnings that must be observed by the user in order to guarantee the safe use of the instrument. Please keep these instructions near the instrument so that they are always to hand when required. 1.2.
1.3 Parts and controls 1.3 Parts and controls 1.3.1 Overall view Safety note The safety guard 2 must always be fitted before operating an 838 Advanced Sample Processor.
1.3.2 Rear panel a Ext. Pump 1 Ext. Pump 2 12 13 W A R N IN G - F i r e H a z a r d - S : 115 V A M SB 1 F o r c o n tin u e d p r o te c tio n r ep la c e o n ly U : 1 0 0 - 24 0 V f: 5 0 - 60 H z w i th th e sa m e t yp e an d ra tin g o f fu s e M a d e by M e tr o h m R e m o te H e r is a u S w itz e r l a n d P ow er 1.838.
1.3 Parts and controls 1.3.
1.3.5 Peristaltic pump The peristaltic pump of the 838 Advanced Sample Processor can be used as a single-channel or two-channel pump. One or two tubing cartridges 6.2755.000 may be fitted. 53 48 52 49 51 50 Figure 7 Peristaltic pump with 2 tubing cartridges 1.3.6 48 Tubing cartridge (6.2755.000) For pump tubing (6.1826.
2.1 General 2 Safety notes Warning! This instrument should only be used in accordance with the information given in these installation instructions. 2.1 General This instrument left our works in perfect condition from the point of view of its operational safety (see Technical data, safety specifications). To keep it in this condition and to continue to operate safely the following information must be carefully observed. 2.
• Mains connection This instrument must only be operated at the specified mains voltages (see rear panel of instrument). 2.3 Personal protection The different drives of the Advanced Sample Processor and the Swing Head possess considerable force in order that they can freely move even under heavy loads. They are fitted with electronic overload limiters that are triggered if the mechanical resistance is too high. When the instrument is running do not intrude in the working range of the Sample Processor.
3.1 Installation flowchart 3 Installation This Chapter describes installation of all components of a Metrohm Sample Processor and illustrates how to connect it to other instruments. Please read the following sections carefully and follow the instructions in detail. This is the only way of ensuring that a Sample Processor will function correctly. 3.1 Installation flowchart The following installation flowchart provides an overview of the required installation work.
Tubing connections Chapter 3.3.8 Ø * Fitting the stand bracket Chapter 3.3.14 Ø * Connecting the pumps Chapter 3.3.15 Ø * Connecting the dosing drive and stirrer Chapter 3.3.16 Ø * Installing the catch pan Chapter 3.3.17 Ø Sample racks Chapter 3.3.18 Ø Fitting the safety guard Chapter 3.3.
3.2 Instrument setup 3.2 Instrument setup 3.2.1 Setup Packaging An 838 Metrohm Advanced Sample Processor and its specially packed accessories are supplied in very protective special packaging consisting of shock-absorbing plastic foam. Please store this packaging in a safe place; it is the only way in which the safe transport of the instrument can be guaranteed.
3.3 Fitting the accessories 3.3.1 Connecting the keypad (Only required if Sample Processor methods are to be used and edited). The 6.2142.050 keypad must be connected to the instrument rear panel at the keypad socket 17 provided. The instrument must be switched off before doing this. Squeeze the connector together at both sides in order to disconnect the connector. Figure 11 Connecting the keypad 3.3.
3.3 Fitting the accessories Please refer to the 786 Swing Head Instructions for Use, which you can obtain from Metrohm by quoting order No. 8.786.1001, for details of the individual settings and robotic arm types. 3.3.3 Fitting the retention plate When sampling from covered vials with the needle, the retention plate 9 serves to wipe the vials when the lift is rising. It is fixed in position on the front side of the tower with the three supplied Allen screws. 9 Figure 13 Fitting the retention plate 3.3.
3.3.5 1 Remove the PEEK pressure screw 24 Undo the PEEK pressure screw 24 screwed onto the needle holder 23 and remove it. 2 Insert the needle • Insert the needle 22 (6.1835.010 PEEK needle or 6.1835.020 PEEK tubing) from the top a little into the opening of the needle holder 23. • Slide the PEEK ring wedge from the top over the needle 22. The narrow side of the seal must point upwards. 3 Secure the needle • Screw the PEEK pressure screw 24 into the needle holder 23.
3.3 Fitting the accessories 3.3.6 Fitting the ultrafiltration cell This section describes initial fitting of the ultrafiltration cell including filtration membrane. Please note Chapter 6.1 when assessing the need to exchange the membrane and when selecting membranes. The procedure for exchanging a filtration membrane is also described in full in Chapter 4.2.2. 28 29 30 31 32 Figure 17 Fitting the ultrafiltration cell 33 34 35 36 28 Ultra filtration cell (6.2729.
2 Preparing the filtration membrane • Remove a new 6.2714.020 filtration membrane 34 from the packaging using the 6.2831.010 tweezers and immerse it for approx. 2 minutes in a petri dish filled with ultra-pure water until the membrane is fully soaked with water. It is imperative that the filtration membrane be soaked with water before inserting it into the filtration cell since this causes it to expand.
3.3 Fitting the accessories ing solution at a special beaker position on the sample rack. • Use the IC NET software to control the 838 Advanced Sample Processor directly. Choose the corresponding system and open the Manual control dialog box. You can run the individual functions of the Sampler Processor under Autosampler. Please refer to the IC NET software Online Help documentation for details of operation. • Move the beaker with the rinsing solution beneath the needle with MOVE.
3.3.7 Fitting the dialysis cell Before fitting the dialysis cell, you must first insert a dialysis membrane. 37 38 39 40 41 42 43 Figure 18 Fitting the dialysis cell 44 43 45 46 47 37 Dialysis cell (6.2729.100) 43 Slot for engaging the dialysis cell in the cell holder 38 Upper section of the dialysis cell 44 Outlet for acceptor solution 39 Sealing ring (E.301.0111) 45 Inlet for acceptor solution 40 Dialysis membrane (e.g. 6.2714.
3.3 Fitting the accessories Only ultra-pure water or ethanol may be used to clean the dialysis cell since other organic solvents (e.g. acetone) would damage the Plexiglass cell! 3 Preparing the dialysis membrane Remove a new dialysis membrane 40 (e.g. 6.2714.010) from the packaging using the 6.2831.010 tweezers and immerse it for approx. 2 minutes in a petri dish filled with ultra-pure water until the membrane is fully soaked with water.
3.3.8 Fitting the Dosino If a Dosino is required, it can be installed in the 6.2057.040 Dosino holder. Fit the Dosino holder as specified in 3.3.5. Figure 20 Fitting the Dosino 22 • Insert the 6.1618.020 threaded adapter (included in the scope of delivery of the 2.838.310) from below into the Dosino holder. • Fit the dosing unit onto the Dosino holder and screw the threaded adapter tight. • Connect the tubing. Figure 21 Threaded adapter GL 45 (6.1618.
3.3 Fitting the accessories 3.3.9 Tubing connections Pump tubing is consumable material whose service life depends on the contact pressure. You should thus fully raise the tubing cartridges by releasing the snap-action lever 51 at the right-hand side if the pump is to be switched off for a long time (this will maintain the set optimum contact pressure). The 6.1826.0X0 pump tubing consists of PVC or PP and may thus not be used for rinsing with solutions containing acetone .
1 Detaching the tubing cartridge(s) Detach the tubing cartridge(s) 48 by pressing in the snapaction lever 51 on the retaining bracket and disengage it (them) from the retaining cam 53. 2 Fitting the pump tubing Press the contact pressure lever 49 of the tubing cartridge(s) fully down. Insert one length of pump tubing into the tubing cartridge(s) as shown above. The stopper 57 must engage in the corresponding fixture at the top of the tubing cartridge.
3.3 Fitting the accessories 3.3.10 Pump tubings Used with Order No. Description Advanced IC Ultrafiltration Sample Processor 2.838.0210: both channels 6.1826.010 Pump tubing made of PVC (Tygon® ST) with 2 permanently mounted white-white stoppers; Inner diameter = 1.02 mm ± 0.05 mm, Delivery 1.41 ml/min (20 rpm) 1.69 ml/min (24 rpm) 150 Pump tubing made of PVC (Tygon® ST) with 2 permanently mounted blue-blue stoppers; Inner diameter = 1.65 mm ± 0.05 mm, Delivery 3.75 ml/min (20 rpm) 4.
Advanced IC Ultrafiltration Sample Processor 2.838.0210: channel for pumping the sample solution 6.1826.070 Optional 6.1826.110 Optional Advanced IC Sample Processor 2.838.0010: both channels 6.1826.120 6.1826.130 Advanced IC Injection Sample Processor 2.838.0020: both channels Advanced IC Dilution Sample Processor 2.838.0120: both channels 6.1826.140 Advanced VA Sample Proc- 6.1826.150 essor 2.838.
3.3 Fitting the accessories 3.3.11 Connection of the ultrafiltration cell 60 PEEK pressure screw (6.2744.010) 56 Pump tubing (6.1826.070) 61 Pump tubing (6.1826.030) 62 PEEK coupling (6.2744.030) 63 PTFE capillary (6.1803.050) 64 PVDF pressure screw (6.2744.000) 65 PTFE capillary (6.1803.060) 66 PTFE capillary (6.1803.080) 67 PEEK capillary (6.1831.
ure 9). • Insert the second end of the capillary into the waste container (e.g. optional: 6.1608.070 bottle with 6.1602.150 bottletop GL45). 3 Filtration cell – pump tubing connection • Fit a PEEK pressure screw 60 to one end of the PTFE capillary 63. • Screw a coupling 62 onto this pressure screw. • Fit a PVDF pressure screw 64 onto the other end of the PTFE capillary.
3.3 Fitting the accessories 3.3.12 Connecting the dialysis cell 76 37 820 70 71 73 64 72 68 A 75 Column Eluent 77 68 833 Pump Unit 69 Metrohm 61 Acceptor 838 74 78 Waste Figure 24 Capillary connections for dialysis 68 PTFE capillary (6.1803.040, 1 m) supply of the sample/acceptor solution 37 Dialysis cell (6.2729.100) 61 Pump tubing (6.1826.030) with orange-yellow stoppers for delivery of the acceptor solution 69 Pump tubing (6.1826.
3.3 Fitting the accessories Proceed as follows in order to connect the dialysis cell 37 to the IC system: Capillaries provided with new connectors must feature a flawless, flat cut edge. In order to ensure this, it is best to use the 6.2621.080 cappillary cutter.. Connections for sample solution 30 1 Fitting the pump tubing to the 838 Advanced Sample Processor Fit the two 6.1826.040 pump tubings (black-black stoppers), as described in Chapter 3.3.
3.3 Fitting the accessories Use only the specified 6.2744.000 PVDF pressure screws for connection to the dialysis cell. If the 6.2744.010 PEEK pressure screws are used, this could cause stress cracking on the dialysis cell! 4 Connecting the waste container Attach the 6.1803.040 PTFE capillary 74 (L =1 m) with a 6.2744.010 PEEK pressure screw to the 6.2744.
3.3 Fitting the accessories Use only the specified 6.2744.000 PVDF pressure screws to connect to the dialysis cell. If the 6.2744.010 PEEK pressure screws are used, this could cause stress cracking on the dialysis cell! 32 4 Connecting the injection valve Screw the free end of the 6.1831.050 PEEK capillary 76 (L = 40 cm) with a 6.2744.010 PEEK pressure screw to connection "1" of the injection valve A 71. Screw the 6.1831.060 PEEK capillary 77 (L =1 m) using a 6.2744.
3.3 Fitting the accessories Conditioning the dialysis system Before the first analysis, the dialysis cell with the fitted dialysis membrane and all tubing connections must be rinsed with ultra-pure water. Please proceed as follows: 1 Settings on the 820 IC Separation Centre In the «IC Net» software, switch injection valve A in the 820 IC Separation Centre to position "FILL".
3.3 Fitting the accessories 3.3.13 Inserting capillaries or tubings The individual chain links of the guide chain can be opened using a screwdriver or a similar implement in order to insert capillaries or tubings. Please note the marking on the chain links. Figure 25 Inserting capilliaries or tubings 3.3.14 Fitting the stand bracket An external dilution or measuring cell can be fitted with the aid of a 6.2001.070 stand bracket on the Advanced Sample Processor.
3.3 Fitting the accessories 3.3.15 Connecting pumps Two external pumps can be connected and controlled on the 838 Advanced Sample Processor. Ext. Pump 2 The pump connections (for 3-pin M8 connectors) on the rear side of the tower of the Advanced Sample Processor supply a voltage of 16 V and may be loaded with max. 600 mA.
3.3 Fitting the accessories 3.3.17 Installing the catch pan The 6.2711.060 catch pan is available as an optional accessory. It prevents escaping fluid spreading over the laboratory bench or penetrating the interior of the instrument. Figure 28 Catch pan Attach the supplied tubing to the drain connector of the catch pan. Detach the sample rack and move the catch pan over the turntable so that it lies on the Sample Processor's stirrer rail. Insert the other end of the tubing into a drain or waste canister.
3.3 Fitting the accessories 3.3.19 Fitting the safety guard/splash guard It is essential to fit the safety guard before using the 838 Advanced Sample Processor for the first time. It not only acts as an effective splash guard but also prevents the risk of injury. The safety guard must be fitted with the securing screw on the side of the tower of the 838 Advanced Sample Processor. The height of the safety guard can be adapted to the sample vessels.
3.4 Connecting external instruments 3.4 Connecting external instruments There are numerous options for connecting the 838 Advanced Sample Processor to Metrohm systems. The 838 Advanced Sample Processor features a serial RS-232 interface via which it is possible to access the instrument functions directly using suitable control software. If working with the sequence methods of the instrument, Sample Processor and connected instruments can be coordinated with control signals via the 25-pin remote interface.
3.4 Connecting external instruments The Metrohm remote cables have a designation at the ends of the cable, indicating for what instrument the relevant connector is scheduled and at what connector position it must be connected. Example: 732/1 Remote 3.4.2 Serial connections (RS232) The connection options at the serial RS232 interface are diverse. Besides all Metrohm instruments featuring the Metrohm remote control language, it is possible to connect a personal computer.
3.5 Connection to Metrohm IC systems 3.5 Connection to Metrohm IC systems 3.5.1 IC system remotely controlled by PC Data acquisition and control of the IC components can be performed from the PC using the «IC Net 2.x» PC software and the 830 IC Interface. In this case, the 838 Advanced Sample Processor is connected to the IC system both via the serial RS232 interface and via the remote interface.
3.5 Connection to Metrohm IC systems 3.5.3 MIC-2 modular anionic system with chemical suppression This system requires the model 2.838.0010 of the 838 Advanced Sample Processor. The 838 Advanced Sample Processor is connected to an anionic IC system with chemical suppression with 819 IC detector, 820 IC Separation Centre (2.820.0230), 818 IC pump, 833 IC Liquid Handling Unit and 830 IC Interface in accordance with Figure 31 using the 6.2141.190 remote cable and the 6.2134.100 RS232 cable. 819 6.2134.
3.5 Connection to Metrohm IC systems 3.5.4 MIC-2 modular anionic system with chemical suppression and sample dilution This system requires the model 2.838.0120 of the 838 Advanced Sample Processor. The 838 Advanced Sample Processor is connected to an anionic IC system with chemical suppression with 819 IC detector, 820 IC Separation Centre, 818 IC pump, 833 IC Liquid Handling Unit and 830 IC Interface in accordance with Figure 32 using the 6.2141.190 remote cable and the 6.2134.100 RS232 cable. 819 6.
3.
3.5 Connection to Metrohm IC systems 3.5.5 Capillary connections for sample dilutions 1 838 Advanced Sample Processor B 820 Separation Centre Waste A 2 G Water 7 H D C Pump F 3 8 4 5 801 E 6 Figure 33 Capilliary connections for sample dilutions A B C D Capillary (6.1803.120) IØ = 0.97 mm, L = 3.0 m Capillary (6.1803.070) IØ = 0.97 mm, L = 0.4 m Capillary (6.1803.070) * IØ = 0.97 mm, L = 0.4 m Capillary (6.1803.080) IØ = 0.97 mm, L = 1.0 m E F G H Capillary (6.1803.060) IØ = 0.
3.5 Connection to Metrohm IC systems 3.5.6 Equipping the work head of the mixing container The 6.1414.070 work head of the mixing container must be provided with a capillary for supply of sample and water and a capillary for aspiration of diluted solution. The other openings must be fitted with blind stoppers. Blind stopper (6.2744.080) Free opening Inlet capilliary Pressure screw (6.2744.010) PEEK capillary (6.1803.070) Aspiration Pressure screw (6.2744.010) PEEK capillary (6.1803.
3.5 Connection to Metrohm IC systems 3.5.7 MIC-2 modular anionic system with chemical suppression and ultrafiltration of the samples This system requires the model 2.838.0210 of the 838 Advanced Sample Processor. Install the ultrafiltration cell, see Chapter 3.3.6.
3.5 Connection to Metrohm IC systems 3.5.8 MIC-3 modular anionic and cationic system with chemical suppression This system requires the model 2.838.0010 of the 838 Advanced Sample Processor. The combined anionic/cationic IC system can operate either with chemical suppression or with electronic suppression.
3.5 Connection to Metrohm IC systems 010 012 013 015 016 Ctrl Scan Ctrl Pump Ctrl 3.5.9 − − − − − ZERO 2 Wait1 FILL A 2 120 s INJECT A 2 Trigger autozero on 819/2 IC Detector Wait for Remote signal Switch over injection valve 2 on 820 to "Fill" Fill sample loop for 120 s with sample Switch over injection valve 2 on 820 to "Inject" MIC-7 anionic system with chemical suppression and dialysis This system requires the model 2.838.0110 of the 838 Advanced Sample Processor.
3.5 Connection to Metrohm IC systems ⇒ The total analysis time per sample comprises the times for dialysis and for the chromatogram. B) Dialysis of the next sample is performed as early as the phase in which one sample is being chromatographically separated. ⇒ The total analysis time per sample is shortened to the time required for one chromatogram.
3.5 Connection to Metrohm IC systems Dialysis.
3.5 Connection to Metrohm IC systems End-Dialysis.
3.5 Connection to Metrohm IC systems 3.5.10 MIC-8 modular system with 817 Bioscan for the sugar analysis This system requires the model 2.838.0020 of the 838 Advanced Sample Processor. The 838 Advanced Sample Processor is connected to a modular Bioscan system with 819 IC detector and 817 Bioscan in accordance with Figure 38 via PC (RS232 connections). 817 6.2125.160 PC 818 838 6.2125.110 6.2125.
3.5 Connection to Metrohm IC systems Injektion valve in FILL position Injektion valve in INJECT position Waste Sample Waste Sample Column Eluent Column Eluent 838-specific settings in the «IC Net 2.
3.5 Connection to Metrohm IC systems 838-specific settings in the «IC Net 2.3» program Program for the 838 Advanced Sample Processor: 001 002 003 Move Lift Pump − Move needle to sample position − Move lift with needle to working height − Fill sample loop with sample Sample work 240 s Time program for 761 Switchover of the injection valve is programmed in the time program of the 761 Compact IC. The times may need to be adapted for the application.
3.5 Connection to Metrohm IC systems 838-specific settings in the «IC Net 2.
3.6 Connection to Metrohm VA systems 3.6 Connection to Metrohm VA systems If the 838 Advanced Sample Processor is operated with a Metrohm VA system, the predefined sequence methods of the 838 Advanced Sample Processor are used. It is absolutely essential to connect the 6.2142.050 keypad in order to adapt these methods. The possible instrument combinations depend greatly on the individual determination methods in voltammetry. Two systems are shown by way of example below. 3.6.
3.6 Connection to Metrohm VA systems 3.6.2 MVA-13 modular system for automatic analysis of electroplating baths The 838 Advanced Sample Processor is connected to a Metrohm 797 VA Computrace with the multiple 6.2141.180 remote cable, see below. Connection of pumps to the 731 Relay Box requires one 6.2160.010 adapter cable in each case. 800 (1) 800 (2) 800 (3) 731 838 797 823 50 mL 2 mL 2 mL 823 6.2141.180 6.2160.010 Figure 42 MVA-13 connection to an analysis system for electroplating baths 3.6.
3.6 Connection to Metrohm VA systems The 6.1835.040 PEEK tubing, the 6.2833.000 needle holder and the 6.1831.070 PEEK capillary can be used for sample transfer of small volumes. Please refer to the 797 VA Computrace Instructions for Use or the documentation of the MVA-13 system for further information.
3.7 Sample racks 3.7 Sample racks A sample rack is a turntable for accommodating sample vessels which is fitted on the Sample Processor. Various types of sample rack can be used and exchanged easily in order to support different types of sample vessel. The rack offers space for different numbers of samples depending on the diameter of the sample vessels. 3.7.1 Metrohm standard sample racks Article No. / Rack type Number of samples Type of sample vessel Vessel diame- Magnetic ter code predefined 6.
3.7 Sample racks 3.7.2 Magnetic codes Each individual sample rack can be uniquely identified by means of a magnetic code. Magnetic pins on the underside of the rack (see Figure 8) can be combined to form a binary, six-digit code. The Sample Processor can thus automatically detect what rack is fitted. When changing the rack, it should first be moved to initial position by pressing the key (if a keypad is connected) or the instrument should be switched off and then on again.
4.1 Servicing 4 Service, maintenance, faults 4.1 Servicing Servicing the Advanced Sample Processor should be carried out within the framework of an annual service visit by technicians from Metrohm or one of its local agencies. If aggressive or corrosive chemicals are used then shorter service intervals are necessary. The Metrohm service department is always pleased to provide competent advice about the servicing and maintenance of all Metrohm instruments. 4.
4.2 Care and maintenance Proceed as follows in order to exchange pump tubing: 1 Removing the old pump tubing • Press the contact pressure lever 49 on the tubing cartridge down fully. • Detach the tubing cartridge 48 from the retaining bracket 50 by pushing the snap-action lever 51 in and disengage the tubing cartridge from the retaining cam 53 on the 838 Advanced Sample Processor. • Remove the old pump tubing. 2 Fitting the new pump tubing • Insert the new pump tubing 56 (6.1826.
4.2 Care and maintenance 4.2.2 Replacing the filtration membrane Perfect condition of the filtration membrane used is absolutely essential in order to achieve constant quality of the analysis results. Consequently, this membrane must be exchanged at regular intervals. Please follow the information in Chapter 6.1 when assessing the need to exchange a membrane. Please proceed as follows (see also Chapter 3.3.
4.2 Care and maintenance Please use only the specified 6.2744.000 PVDF pressure screws for connection to the ultrafiltration cell. If you use the 6.2744.010 PEEK pressure screws, this may result in stress cracking on the ultrafiltration cell! 64 4 Closing the ultrafiltration cell • Fit the lower section 45 onto the upper section 43 so that the two parts fit perfectly. • Using the 6.2621.070 Allen key, fully screw in and firmly tighten the 5 screws 46.
4.2 Care and maintenance 4.2.3 Replacing the dialysis membrane It may be necessary to replace the dialysis membrane in the following cases: • Reduced yield in dialysis • Dried-out membrane or membrane damage as the result of deposits or bacterial growth • Irremediable blockage of the sample channel (sample is no longer able to be pumped through the dialysis cell) Proceed as follows in order to exchange the membrane (see also 3.3.7): 1 Detaching the dialysis cell Unscrew the four inlet resp.
5.1 Validation / GLP 5 GLP validation – diagnosis 5.1 Validation / GLP GLP (Good Laboratory Practice) requires, among other things, that the precision and correctness of analytical instruments is checked at regular intervals by using SOPs (Standard Operating Procedures). As this instrument is not a measuring instrument as such, we recommend that the Sample Processor is included in an analytical system and is validated as part of the all-embracing validation of the whole analytical system.
6.1 Filtration 6 Annex 6.1 Filtration 6.1.1 Selection of possible sample types Each filtration process using a filtration membrane with a small pore size could be subject to a membrane blockage. The following table lists some types of sample that have been filtered with the Metrohm 6.2729.110 Ultrafiltration Cell with the standard 6.2714.020 Filtration membrane (0.15 μm) or a second filter membrane (0.2 μm) and then analyzed on a Metrohm IC system.
6.1 Filtration 6.1.2 Filter working life A reduction in the recovery rate when analyzing standard solutions can be used as a possible indicator for the early recognition of an impending blockage. Ideally these solutions should be made up in the sample matrix being analyzed. This means that if a large number of samples are to be analyzed then it is advisable to measure standard solutions at regular intervals, for example after every 5th or 10th sample for sample with a high particle load.
6.1 Filtration Test solutions: silica particles in water (1) (2) Pore size of filter membrane 1 Effect 0.5 %, 5 μm 0.15 μm no breakthrough 0.5 %, 5 μm 3 μm no breakthrough 0.5 %, 5 μm 8 μm no breakthrough 0.5 %, 5 μm 10 μm breakthrough 2 0.5 %, 5 μm 12 μm no breakthrough 0.5 %, 1.5 μm 0.15 μm no breakthrough 0.5 %, 1.5 μm 3 μm breakthrough Nominal pore size according to information supplied by manufacturer. All the membranes came from a single manufacturer except this one.
6.2 Technical data 6.2 Technical data 6.2.1 6.2.2 6.2.3 2 Channel peristaltic pump Speed ± 6.7/min ... ± 100/min adjustable in ±15 steps Delivery rate (typical) Tubing Delivery (with water, no backpressure, dependent on contact pressure and tubing type, at 20/min apart from 6.1826.150 in the case of 67/min) 6.1826.010: 6.1826.020: 6.1826.030: 6.1826.040: 6.1826.070: 6.1826.130: 6.1826.150: 1.4 ml/min 3.8 ml/min 0.4 ml/min 0.8 ml/min 2.6 ml/min 1.4 ml/min 23.8 ml/min Pressure max. 4 bar (0.
6.2 Technical data 6.2.
6.2 Technical data 6.2.12 Safety specification Design and testing in accordance with EN/IEC 61010-1, EN/IEC 61010-2-081, UL 3101-1 safety class I Safety notes The Instructions for Use contain information and warnings which must be followed by the user in order to ensure safe operation of the instrument. 6.2.
6.2 Technical data 6.2.15 Dimensions and material Height Width Depth 0.73 m 0.28 m 0.50 m 15.50 kg (1.838.0010) Weight (not including accesso- 15.90 kg (1.838.
6.3 Standard equipment 6.3 Standard equipment On receipt of the instrument please check that the delivery is complete. 6.3.1 Metrohm 838 Advanced Sample Processor Order no. 2.838.0010 Advanced IC Sample Processor The following accessories are included as standard: No. Order no. 1 1.838.0010 1.786.0010 6.1462.
6.3 Standard equipment 1 6.2064.000 Retention plate 1 6.2134.100 RS-232 cable DB9/f — DB9/f (5 m) 1 6.2141.190 1 6.2621.300 Remote cable 732/819 — 766/788/838 Tool kit for 838 1 6.2739.000 Spanner for nipple 1 6.2744.010 Pressure screw made of PEEK with UNF 10/32 connection, 5 ea. 1 6.2744.034 Coupling, olive/UNF 10/32 2 ea. 1 6.2744.160 Coupling with lock Olive/UNF 10/32 1 6.2751.110 Safety guard/splash guard 1 Y.1070.150 Cable strap, self-adhesive 1 6.2122.
6.3 Standard equipment Order No. 2.838.0020 Advanced IC Injection Sample Processor The following accessories are included in the scope of delivery: Qty. Order No. Description 1 1.838.0020 838 Advanced Sample Processor For Metrohm IC systems 1.786.0010 6.1462.090 • • • • • • 1 tower Swing Head Robotic arm with needle adapter Peristaltic pump Injection valve Without sample rack Sample racks, see 6.3.2. 3 6.1608.080 PE bottle 300 ml (40 mm opening) 1 6.1825.
6.3 Standard equipment 1 6.2064.000 Retention plate 1 6.2134.100 RS-232 cable DB9/f — DB9/f (5 m) 1 6.2141.190 Remote cable 732/819 — 766/788/838 1 6.2621.300 Tool kit for 838 1 6.2739.000 Spanner for nipple 1 6.2744.010 Pressure screw made of PEEK with UNF 10/32 connection, 5 ea. 1 6.2744.034 Coupling, olive/UNF 10/32 2 ea. 1 6.2744.110 Coupling UNF 10/32 external / olive 1 6.2744.160 Coupling with lock Olive/UNF 10/32 1 6.2751.110 Safety guard/splash guard 1 Y.1070.
6.3 Standard equipment Order No. 2.838.0110 Advanced IC Dialysis Sample Processor The following accessories are included in the scope of delivery: Qty. Order No. Description 1 1.838.0010 838 Advanced Sample Processor For Metrohm IC systems 1.786.0010 6.1462.090 • • • • • • 1 tower Swing Head Robotic arm with needle adapter Peristaltic pump Without injection valve Without sample rack Sample rack, see 6.3.2. 1 6.1602.150 Bottletop GL 45-3x10/32 3 6.1608.
6.3 Standard equipment 1 6.1831.050 PEEK capillary 1/16 " d = 0.5 mm, L = 0.40 m 1 6.1831.060 PEEK capillary 1/16 " d = 0.5 mm, L = 1 m 1 6.1835.010 PEEK needle 1/8" 1 6.2057.010 Holder for dialysis cell 6.2729.100 1 6.2064.000 Retention plate 1 6.2134.100 RS-232 cable DB9/f — DB9/f (5 m) 1 6.2141.190 Remote cable 732/819 — 766/788/838 1 6.2621.300 Tool kit for 838 1 6.2714.010 Dialysis membrane made of cellulose acetate 0.2 μm, 50 ea. 1 6.2729.
6.3 Standard equipment 1 6.2744.000 Pressure screw made of PVDF, UNF 10/32 5 ea. 1 6.2744.010 Pressure screw made of PEEK with UNF 10/32 connection, 5 ea. 1 6.2744.034 Coupling, olive/UNF 10/32 2 ea. 1 6.2744.160 Coupling with lock Olive/UNF 10/32 1 6.2751.110 Safety guard/splash guard 1 6.2831.010 PBTP tweezers 1 Y.1070.150 Cable strap, self-adhesive 1 6.2122.xxx Mains cable with coupling, Type IEC 320/C13 Cable connector to customer specifications: - Type SEV 12 (Switzerland...) 6.
6.3 Standard equipment Order No. 2.838.0120 Advanced IC Dilution Sample Processor The following accessories are included in the scope of delivery: Qty. Order No. Description 1 1.838.0020 838 Advanced Sample Processor For Metrohm IC systems 1.786.0010 6.1462.090 • • • • • • 1 tower Swing Head Robotic arm with needle adapter Peristaltic pump With injection valve Without sample rack Sample racks, see 6.3.2. 1 6.1414.070 Titration vessel upper section 1 6.1456.210 Mixing vessel 10 … 90 ml 3 6.
6.3 Standard equipment 3 6.1608.080 PE bottle 300 ml (40 mm opening) 1 6.1619.010 Absorber 1 6.1803.060 PTFE capillary 1/16 " d = 0.97 mm, L = 0.20 m 1 6.1803.070 PTFE capillary 1/16 " d = 0.97 mm, L = 0.40 m 1 6.1803.080 PTFE capillary 1/16 " d = 0.97 mm, L = 1.0 m 1 6.1803.110 PTFE capillary 1/16 " d = 0.97 mm, L = 0.50 m 1 6.1803.120 PTFE capillary 1/16 " d = 0.97 mm, L = 3.0 m 2 6.1826.140 Pump tubing LFL Grey/Grey 1 6.1835.010 PEEK needle 1/8" 1 6.2001.
6.3 Standard equipment 1 6.2036.000 Retaining ring for titration vessel 1 6.2064.000 Retention plate 1 6.2134.100 RS-232 cable DB9/f — DB9/f (5 m) 1 6.2141.190 Remote cable 732/819 — 766/788/838 1 6.2621.300 Tool kit for 838 1 6.2730.030 Stopper with nipple M10 1 6.2739.000 Spanner for nipple 1 6.2744.010 Pressure screw made of PEEK with UNF 10/32 connection, 5 ea. 1 6.2744.034 Coupling, olive/UNF 10/32 2 ea. 1 6.2744.
6.3 Standard equipment 1 6.2744.080 PEEK coupling, M6 external/UNF 1 6.2744.160 Coupling with lock Olive/UNF 10/32 1 6.2751.110 Safety guard/splash guard 1 Y.1070.150 Cable strap, self-adhesive 1 6.2122.xxx Mains cable with coupling, Type IEC 320/C13 Cable connector to customer specifications: - Type SEV 12 (Switzerland...) 6.2122.020 - Type CEE(7), VII (Germany...) 6.2122.040 - Type NEMA/ASA (USA...) 6.2122.070 1 84 8.838.
6.3 Standard equipment Order No. 2.838.0210 Advanced IC Ultrafiltration Sample Processor The following accessories are included in the scope of delivery: Qty. Order No. Description 1 1.838.0010 838 Advanced Sample Processor For Metrohm IC systems 1.786.0010 6.1462.090 • • • • • • 1 tower Swing Head Robotic arm with needle adapter Peristaltic pump Without injection valve Without sample rack Sample racks, see 6.3.2. 3 6.1608.080 PE bottle 300 ml (40 mm opening) 1 6.1803.
6.3 Standard equipment 2 6.1831.060 PEEK capillary 1/16 " d = 0.5 mm, L = 1 m 1 6.1835.010 PEEK needle 1/8" 1 6.2057.030 Filtration cell holder 1 6.2064.000 Retention plate 1 6.2134.100 RS-232 cable DB9/f — DB9/f (5 m) 86 1 6.2141.190 Remote cable 732/819 — 766/788/838 1 6.2621.300 Tool kit for 838 1 6.2714.020 Filtration membranes 0.2 μm, 50 ea. 1 6.2729.110 Ultrafiltration cell 1 6.2739.
6.3 Standard equipment 1 6.2744.000 Pressure screw made of PVDF, UNF 10/32 5 ea. 1 6.2744.010 Pressure screw made of PEEK with UNF 10/32 connection, 5 ea. 1 6.2744.030 Coupling, olive/UNF 10/32 4 ea. 1 6.2744.160 Coupling with lock Olive/UNF 10/32 1 6.2751.110 Safety guard/splash guard 1 6.2831.010 PBTP tweezers 1 Y.1070.150 Cable strap, self-adhesive 1 6.2122.xxx Mains cable with coupling, Type IEC 320/C13 Cable connector to customer specifications: - Type SEV 12 (Switzerland...) 6.
6.3 Standard equipment Order No. 2.838.0310 Advanced VA Sample Processor The following accessories are included in the scope of delivery: Qty. Order No. Description 1 1.838.0010 838 Advanced Sample Processor For Metrohm VA systems 1.786.0010 6.1462.090 • • • • • 1 tower Swing Head Robotic arm with needle adapter Peristaltic pump Without injection valve 1 6.1618.020 Threaded adapter S40 to GL45 1 6.1805.020 FEP tubing connection M6 d = 2 mm, L = 0.52 m 1 6.1805.
6.3 Standard equipment 1 6.1822.420 PEEK capillary 1x M6, 1/16" d = 0.75 mm 2 6.1826.020 Pump tubing ENE-18 Blue/Blue 2 6.1826.150 Pump tubing ENE-23 Violet/Orange 1 6.1831.070 PEEK capillary 1/16" d = 0.75 mm, L = 0.70 m 1 6.1831.080 PEEK capillary 1/16" d = 0.75 mm, L = 1.10 m 1 6.1835.040 PEEK needle 1/8" 1 6.1835.050 PEEK needle 1/8" d = 1.58 mm 1 6.2041.450 Sample rack 56 x 15 ml + 56 x 50 ml 1 6.2057.040 Dosino holder 1 6.2142.050 Keypad for 838 1 6.2621.
6.3 Standard equipment 1 6.2739.000 Spanner for nipple 1 6.2743.057 PP sample vessels 11 ml 200 ea. 1 6.2744.010 Pressure screw made of PEEK with UNF 10/32 connection, 5 ea. 2 6.2744.080 Coupling M6 external/UNF 10/32 internal 1 6.2744.160 Coupling with lock Olive/UNF 10/32 4 6.2747.010 PP sample vessels 50 ml 25 ea. 90 1 6.2751.110 Safety guard/splash guard 1 6.2833.020 Needle holder 1/8" to M6 2 Y.1070.150 Cable strap, self-adhesive 1 6.2122.
6.3 Standard equipment 6.3.2 6.3.3 6.3.4 Keyboard (optional) Order No. Description 6.4142.050 Keyboard for the 838 Advanced Sample Processor Dosing drive and units Order No. Description 2.800.0010 800 Dosino 6.3032.120 807 Dosing unit, 2 mL glass cylinder 6.3032.150 807 Dosing unit, 5 mL glass cylinder 6.3032.210 807 Dosing unit, 10 mL glass cylinder 6.3032.220 807 Dosing unit, 20 mL glass cylinder 6.3032.250 807 Dosing unit, 50 mL glass cylinder Stirrer Order No. 2.801.
6.3 Standard equipment 6.3.5 Sample racks and vessels Order No. Description 6.2041.310 Sample rack 12 x 250 ml (d = 416 mm) for 6.1432.320 6.1453.220 6.1453.250 Metrohm sample beakers, glass, 250 ml Metrohm sample beaker PP 200 ml Metrohm sample beaker PP 250 ml 6.2041.320 Sample rack 16 x 150 ml for Standard beakers (high type) 6.2041.340 Sample rack 24 x 75 ml (d = 416 mm) for 6.1432.210 Metrohm sample beakers, glass 75 ml, 6.2041.350 6.1432.
6.3 Standard equipment 6.2041.450 6.2743.057 6.2747.010 6.2041.760 6.2743.057 6.1432.320 6.1453.220 6.1453.250 Sample rack 56x 11 ml + 56x 50 ml (d = 476 mm,) for sample tubes 11 ml and PP sample vessel 50 ml Sample rack 54x 11 ml + 1x 250 ml (d = 476 mm,) for sample tubes 11 ml and Metrohm sample beakers, glass, 250 ml or Metrohm sample beakers, PP 200 ml or Metrohm sample beakers, PP 250 ml 6.2743.040 PP sample vessels 2.5 mL 2000 ea., not for use with ultra filtration cell 6.2743.
6.3 Standard equipment 94 1 6.1462.030 Robotic arm for sample transfer, for left-swinging installation 1 6.1562.100 Pipetting tubing 10 ml 1 6.1823.010 Guide shank for pipetting tubing 1 6.2621.120 Allen key 1.5 mm 1 6.2751.100 Splash guard 2 V.024.4012 Countersunk screws M.
6.3 Standard equipment 6.3.7 Connecting cables Order No. Description 6.2141.020 Standard remote cable 25-pin/25-pin 6.2134.090 Remote cable 762/830 IC Interface — Device 1...4 or 5... 6.2141.110 Remote cable 766/788/813 — 709 or 762 and 732 6.2141.140 Remote cable 766/788/813 — 817 6.2141.180 Remote cable 797— 766/813/838 — 731, 25-pin/25-pin/25pin 6.2125.090 Connecting cable RS232 Wired 1:1, 1m, 25-pin/m to 25-pin/m 6.2125.
6.3 Standard equipment 6.3.9 Pump tubing Order No. 6.1826.010 6.1826.020 6.1826.020 6.1826.030 6.1826.040 6.1826.050 6.1826.060 6.1826.070 6.1826.110 96 Description Pump tubing made of PVC (Tygon® ST), White/White Inner diam. = 1.02 mm ± 0.05 mm Pump tubing made of PVC (Tygon® ST), Blue/Blue Inner diam.= 1.65 mm ± 0.05 mm Pump tubing made of PVC (Tygon® ST), Blue/Blue Inner diam.= 1.65 mm ± 0.05 mm Pump tubing made of PVC (Tygon® ST), Orange/Yellow Inner diam.= 0.51 mm ± 0.
6.3 Standard equipment 6.1826.120 6.1826.130 6.1826.140 6.1826.150 Long-life pump tubing made of PVC (Tygon® LFL), Orange/White Inner diam.= 0.59 mm ± 0.05 mm Long-life pump tubing made of PVC (Tygon® LFL), White/White Inner diam.= 1.02 mm ± 0.0127 mm Long-life pump tubing made of PVC (Tygon® LFL), Grey/Grey Inner diam.= 0.51 mm ± 0.0102 mm Pump tubing made of PVC (Tygon® ST), Violet/Orange Inner diam.= 2.54 mm 150 400 150 400 150 400 150 400 6.3.10 Needles and tubings Order No.
6.4 Warranty 6.4 Warranty The warranty on our products is limited to defects that are traceable to material, construction or manufacturing error which occur within 12 months from the day of delivery. In this case the defects will be rectified in our workshops free of charge. Transport costs are to be paid by the customer. For day and night operation the warranty is limited to 6 months. Glass breakage in the case of electrodes or other parts is not covered by the warranty.
6.5 Declaration of Conformity 6.5 Declaration of Conformity This is to certify the conformity to the standard specifications for electrical appliances and accessories, as well as to the standard specifications for security and to system validation issued by the manufacturing company. Name of commodity 838 Advanced Sample Processor Description CH-9101 Herisau/Switzerland E-Mail info@metrohm.com www.metrohm.
6.5 Declaration of Conformity 6.5.1 Quality Management Principles Metrohm AG, CH-9101 Herisau, Switzerland CH-9101 Herisau/Switzerland E-Mail info@metrohm.com Internet www.metrohm.com Metrohm Ltd. holds the ISO 9001 Certificate, registration number 10872-02, issued by SQS (Swiss Association for Quality and Management Systems). Internal and external audits are carried out periodically to assure that the standards defined by Metrohm’s QM Manual are maintained.
7 Index 7 Index .............................. 60 731 Relay Box ................. 56, 57 761 Compact IC........ 21, 40, 53 772 Pump Unit ...................... 35 797 VA Computrace........ 56, 57 820 IC Separation Centre21, 33 823 Membrane Pump Unit.... 35 833 Dialysis Unit ............. 31, 33 A ________________________ Acceptor solution...... 29, 31, 33 Accessories .. 76, 78, 81, 85, 88 Acetone........................... 23, 62 Adapter cable (6.2160.010) .... ...................................
7 Index Inserting a dialysis membrane20 Inserting capillaries............... 34 Inserting the filtration membrane ......................................... 18 Installation....................... 11, 13 Instrument models.................. 2 Instrument number ................. 7 Interconnection ..................... 38 797 VA Computrace......... 56 analysis system for electroplating baths (MVA-13)...................... 57 anionic system with chemical suppression (MIC-2).............
7 Index Signals .................................. 39 Simultaneous dialysis ........... 49 Snap-action lever ........ 8, 23, 24 Solvents ................................ 35 SOP....................................... 66 Splash guard ........................ 37 Stand bracket ....................... 34 Standard equipment ............. 74 Standard sample racks......... 59 Standards ............................. 72 Steel needle .......................... 96 Stirrer connection.................... 6 Stirrer rail..
