Instruction Manual

8.8 Calibration curve technique
797 VA Computrace – Software
249
2. Click on or MAIN WINDOW / Window / Working method
specification
to open the WORKING METHOD SPECIFICATIONS
window.
3. Load the desired method into the WORKING METHOD SPECIFI-
CATIONS
window (see How to Load a method, section 8.4).
4. Select Record calibration curve in the Calibration field of the
WORKING METHOD SPECIFICATIONS window (is not selectable
with the CVS and CPVS mode).
5. Select Manual in the Addition field of the WORKING METHOD
SPECIFICATIONS
window.
6. Select Batch in the Technique field of the WORKING METHOD
SPECIFICATIONS
window.
7. Click to open the EDIT WORKING METHOD PA-
RAMETERS
window.
8. Select the Determination tab (see section 5.2) and enter the Cell
volume
and the number of additions in the No. of additions
field.
9. Select the Substances tab (see section 5.2) and make sure that
for each substance entered in the table the number of the sin-
gle or mixed standard solution, its concentration and its volume
is defined.
10. If the addition of the standard solution should be done with
variable addition volumes, click the button in the Volume
column to open the EDIT VARIED ADDITION window, enter the
variable addition volumes in the Addition fields, and close this
window by clicking <OK>.
11. Close the EDIT WORKING METHOD PARAMETERS window by
clicking <OK>.
12. Place the electrolyte solution (e.g. buffer) in the measuring ves-
sel at the 797 VA Computrace stand.
13. Click on or MAIN WINDOW / Window / Monitor to open
the MONITOR window.
14. Start the measurement by clicking the icon in the MAIN
WINDOW
or the button in the MONITORING win-
dow.
15. Enter the Calibration curve id (used as part of the determination
file name) in the START CALIBRATION window and click <OK>.
16. Each time an addition is required in the MANUAL ADDITION
window, add the standard solution using a pipette.
Record calibration curve manually with solution exchange
This method is used for recording a calibration curve using different
calibration solutions of known concentration. Proceed as follows: