00-120V USE ONL Y WITH 250V DES F USIB LE S DE 250V UNIQUE ME NT A VE C FUS ES/E MP LOY E R Metrohm 220-240V 788 IC Filtration Sample Processor Instructions for Use 8.788.
CH-9101 Herisau/Switzerland Phone +41 71 353 85 85 Fax +41 71 353 89 01 E-Mail info@metrohm.com Internet www.metrohm.com 788 IC Filtration Sample Processor Instructions for Use 8.788.1003 03.
Table of contents Table of contents 1 Introduction ..................................................................................... 1 1.1 Instrument description ................................................................... 1 1.2 Parts and controls ........................................................................... 2 1.3 Information about the Instructions for Use ................................. 5 1.3.1 Organization .......................................................................
Table of contents 2.4 Installation for full control with «IC Net»................................... 23 2.4.1 Connection options.............................................................. 23 2.4.2 General information on interface connections..................... 24 2.4.3 Metrohm IC Systems ........................................................... 25 2.4.4 MIC 1 – Modular anion or cation system with electronic suppression .................................. 26 2.4.
Table of contents 4 Operation ......................................................................................... 53 4.1 Fundamentals of operation.......................................................... 53 4.1.1 Display.................................................................................. 53 4.1.2 Keyboard.............................................................................. 54 4.1.3 Overview of key functions .................................................... 55 4.1.
Table of contents 5 Interfaces .................................................................................... 111 5.1 Remote interface ......................................................................... 111 5.1.1 Pin assignment of the remote socket .................................111 5.1.2 Functional characteristics ...................................................112 5.2 RS232 interface ........................................................................... 115 5.2.
Table of contents List of figures Fig. 1: Side view of the 788 IC Filtration Sample Processor ........................................2 Fig. 2: Rear of the 788 IC Filtration Sample Processor ................................................4 Fig. 3: Setting the mains voltage ................................................................................10 Fig. 4: Keyboard connection ......................................................................................12 Fig.
Table of contents VI 788 IC Filtration Sample Processor
1.1 Instrument description 1 Introduction 1.1 Instrument description The 788 IC Filtration Sample Processor can be used for automating ion chromatographic determinations including in-line filtration, especially in combination with Metrohm IC system instruments. It is possible to apply and automate existing procedures for sample preparation using the ultra-filtration of the 788 IC Filtration Sample Processor.
1 Introduction 1.2 Parts and controls 5 6 7 8 9 10 4 3 11 2 12 1 13 14 15 16 17 18 19 6 20 12 21 22 23 24 25 22 22 Always install splash protection! 26 220-240V 100-120V DES FUSIBLES DE 250V UNIQUEMENT AVEC FUSES/EMPLOYER USE ONL Y WITH 250V Metrohm 27 28 36 35 29 34 30 33 32 Always install plug cover! 31 Fig.
1.2 Parts and controls 1 Splash protection (6.2751.040) Must be installed always in operation! 19 PEEK coupling (6.2744.030) (Olive) Connecting compression fitting and pump tubing 2 Needle PEEK needle (6.1835.010) or PEEK tubing (6.1835.020) 20 PEEK capillary (6.1831.060) (1 m) For conveying the filtrate into the sample loop 3 Lift With swing head attached 21 PTFE capillary (6.1803.050) (0.5 mm / 0.2 m) Connecting ultrafiltration cell and filtrate pump tubing 4 Needle guide (4.766.
1 Introduction 9 37 37 14 27 1 37 37 23 36 26 28 28 35 38 Type 1.788. RS 232 39 Keyboard Made by Metrohm Herisau Switzerland 31 40 Fig. 2: Rear of the 788 IC Filtration Sample Processor 4 1 Splash protection (6.2751.040) Must be installed always in operation! 35 Sample rack 6.2041.430 9 Guide chain For fixing tubings and cables 36 PP sample tube (6.2743.050) (11 mL) Can be sealed with 6.2743.070 PP caps 14 Tubing cartridges (6.2755.000) For 6.1826.
1.3 Information about the Instructions for Use 1.3 Information about the Instructions for Use Please read through these Instructions for Use carefully before operating the 788 IC Filtration Sample Processor. The Instructions for Use contain information and warnings to which the user must pay attention in order to assure safe operation of the instrument.. 1.3.1 Organization These 8.788.
1 Introduction 1.3.
1.4 Support documentation 1.4 Support documentation 1.4.1 Application Bulletins The «Application Bulletin» is a collection of analytical methods, application examples and literature references. All these Application Bulletins are available on request free of charge from your Metrohm supplier. You will find an updated list of the Application Bulletins at any time under «http://www.metrohm.com». 1.4.2 Application Notes The «Application Notes» present application information in concentrated form, i.e.
1 Introduction 1.5.2 General safety rules x Install splash protection To avoid any danger of injury by the needle, the splash protection must always be installed when operating the 788 IC Filtration Sample Processor! x Install plug cover To prevent any contamination of the mains and remote connection by spilled solvents or chemicals, the plug cover must always be installed when operating the 788 IC Filtration Sample Processor! x Do not use caps together with the PEEK tube If you are using the 6.1835.
2.1 Setting up the instrument 2 Installation 2.1 Setting up the instrument 2.1.1 Packaging The 788 IC Filtration Sample Processor is supplied together with the separately packed accessories in special packaging containing shockabsorbing foam linings designed to provide excellent protection. The actual instrument is packed in an evacuated polyethylene bag to prevent the ingress of dust. Please store all these special packagings as only they can assure damage-free transport of the instrument. 2.1.
2 Installation 2.2 Mains connection Follow the instructions below for connecting to the power supply. If the instrument is operated with the mains voltage set wrongly and/or wrong mains fuse there is a danger of fire! 2.2.1 Setting the mains voltage 220 – 240 V 100 – 120 V 100 - 120 V 100 - 120 V 220 - 240 V 34 220 - 240 V 32 Fuse holder 33 Mains connection plug 34 Mains switch 33 32 Fig.
2.2 Mains connection 2.2.2 4 Insert fuse Change fuse if necessary and reinsert in fuse holder 32. 32 5 Install fuse holder Depending on the desired mains voltage, insert fuse holder 32 in the 788 IC Filtration Sample Processor so that the corresponding mains voltage range can be read normally and the adjacent white arrow points to the white bar imprinted on the right side of the fuse holder (see Fig. 3). Fuses One of the two fuses 0.5 A/slow-blow for 100}120 V or 0.
2 Installation 2.3 Attaching the accessories For attaching the accessories at the 788 IC Filtration Sample Processor, proceed in the order described below. 2.3.1 Connecting the swing head Plug in the branch plug of the connection cable 29 permanently attached to the swing head into the remote connection socket at the 788 IC Filtration Sample Processor and screw it onto this connection using a screw-driver (see Fig. 1). 2.3.2 Connecting the keyboard 1 Connecting the keyboard Connect the 6.2142.
2.3 Attaching the accessories 2.3.4 Installing the splash protection To avoid any danger of injury by the needle, the 6.2751.040 splash protection must always be installed when operating the 788 IC Filtration Sample Processor! 1 Remove holding screws Remove the holding screws 37 and the washer mounted on the screw threads 12 at tower 27 using the 6.2621.100 Allen key. 2 Remove protective film from splash protection Remove the plastic film glued on both sides of the splash protection 1.
2 Installation 2 PEEK needle (6.1835.010) or PEEK tubing (6.1835.020) 4 Needle holder 5 PEEK compression fitting (4.766.4320) 6 PEEK compression fitting (6.2744.010) 4 7 Swing head 2 8 PTFE capillary (6.1803.070) 8 7 6 5 Fig. 6: Needle installation If you are using the 6.1835.020 PEEK tubing, the sample tubes may not be sealed with caps because they cannot be pierced by the PEEK tubing and the needle may be damaged thereby. 2.3.
2.3 Attaching the accessories 2 PEEK needle (6.1835.010) or PEEK tubing (6.1835.020) 2 41 35 Sample rack (6.2041.430) 41 Adjusting position 37 42 42 Adjusting screw 35 Fig. 8: Adjusting the sample rack 788 IC Filtration Sample Processor 2 Move to adjusting position Press , enter number '37' and confirm with . Sample rack and swing head are turned until needle is above the adjusting position 37 (first opening of the middle row).
2 Installation 2.3.8 Assembling the ultra-filtration cell In this section, the assembling of the ultra-filtration cell including the filtration membrane is described. Please consider section 4.7 for evaluating the membrane type and time of exchange. The exchange of filtration membranes is also described in section 6.3.4. 23 43 47 44 50 45 48 46 49 Fig. 9: Assembling the ultra-filtration cell 23 Ultra-filtration cell (6.2729.110) 46 Screws V.022.6030 incl. washer 4.754.
2.3 Attaching the accessories Use only ultrapure water or ethanol to clean the dialysis cell; organic solvents (e.g. acetone) will cause damage to the plexiglas cell! 2 Prepare filtration membrane x Use the 6.2831.010 tweezers to extract a new filtration membrane 50 (6.2714.020) from its packaging and immerse in a Petri dish containing ultra pure water for approx. 2 min until the membrane is completely saturated with water.
2 Installation 6 Rinse ultra-filtration cell x Before rinsing the cell, please finish all tubing connections as described in the following sections. x Every time the filtration membrane is changed, the remaining air in the cell and tubings has to be removed. This can be done by rinsing with ultrapure water. x A completely installed 788 IC Filtration Sample Processor with ultra-filtration cell is required for processing the following steps. The rinsing solution (e.g.
2.3 Attaching the accessories 2.3.9 Tubing connections The tubing connections on the 788 IC Filtration Sample Processor and the connection to the injection valve of the 733 IC Separation Center must be made as follows: Pump tubings are consumable material with a lifetime which depends on the contact pressure (see chapter 6.3.3).
2 Installation 20 1 Install pump tubing x Release tubing cartridge 14 from holding clamp 17 by pressing down snap-action lever 16 and remove from mounting pin 13 on the 788 IC Filtration Sample Processor (see Fig. 1). x Press contact pressure lever 18 on the tubing cartridge down as far as it will go. x Insert pump tubings 10 and 11 in the tubing cartridges 13 as shown in Fig. 10. The black-black stopper 43 must click into the corresponding holder on the left-hand side of each tubing cartridge.
2.3 Attaching the accessories Do not connect the dialysis cell with anything other than the 6.2744.000 PVDF compression fittings supplied. If 6.2744.010 PEEK compression fittings are used, stress cracks may appear in the ultra-filtration cell ! 788 IC Filtration Sample Processor 3 Connection pump tubing 10 – ultra-filtration cell x Mount a PEEK compression fitting 6 on the end of the PTFE capillary 24 and screw it on to coupling 19.
2 Installation x Pull the PEEK capillary 20 through the opening 22 or 28 of the 733 IC Separation Center and screw onto connection "1" of injection valve 66 using a 6.2744.010 PEEK compression fitting 6. x Retighten rotary nipple on the interior side of connection 22 or 28 to fix the capillary 20. 20 7 Tubing connection injection valve – waste x Insert 6.2744.020 coupling (from 733 accessories) into connection 21 or 27 of the 733 IC Separation Center. x Screw PTFE suction tubing 84 onto the 6.2744.
2.4 Installation for full control with «IC Net» 2.4 Installation for full control with «IC Net» 2.4.1 Connection options The 788 IC Filtration Sample Processor can be connected to Metrohm IC Systems according to the following techniques: 1. The IC System is totally controlled by the PC software Data acquisition and direct control of the IC components is done by the PC software «IC Net 2.1» in combination with the 762 IC Interface.
2 Installation 2.4.2 General information on interface connections Before an external device is connected to remote connection 30 or RS232 interface 40, 40 the 788 IC Filtration Sample Processor must always be switched off using mains switch 34! 34 Remote connection The branch plug of cable 29 leading from the swing head (see Fig. 1) is plugged into the 25 pin remote interface (see section 2.3.1). Any external devices can be connected to remote connection 30 of this branch plug.
2.4 Installation for full control with «IC Net» 2.4.3 Metrohm IC systems Metrohm IC systems can be classified into modular IC systems ("MIC") and compact IC systems (e.g. 761 Compact IC or 790 Personal IC).
2 Installation 2.4.4 MIC 1 Modular anion or cation system with electronic suppression The 788 IC Filtration Sample Processor is connected to an IC system with electronic suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump and 762 IC Interface as shown in Fig. 13 using the 6.2141.110 remote connection cable and the 6.2134.080 RS232 cable. 732 6.2128.130 733 6.2125.090 6.2141.110 6.2115.070 PC 6.2134.100 762 709 788 6.2134.080 6.2134.090 Fig.
2.4 Installation for full control with «IC Net» 2.4.5 MIC 2 Modular anion system with chemical suppression The 788 IC Filtration Sample Processor is connected to an anion IC system with chemical suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump, 752 IC Pump Unit, and 762 IC Interface as shown in Fig. 14 using the 6.2141.110 remote connection cable and the 6.2134.080 RS232 cable. 732 6.2128.130 6.2125.090 733 6.2134.090 6.2141.110 6.2115.070 709 PC 6.2134.
2 Installation 2.4.6 MIC 3 Modular anion and cation system with chemical suppression The modular anion and cation system can be operated with chemical or electronic suppression. The 788 IC Filtration Sample Processor is connected to the MIC 3 system consisting of two 732 IC Detectors, 733 IC Separation Center (two injectors), two 709 IC Pumps, 753 IC Suppressor Module (if suppression is used), and the 762 IC Interface as shown in Fig. 15 using the 6.2125.120 remote adapter, the 6.2125.
2.4 Installation for full control with «IC Net» Settings in the «IC Net 2.1» program 1. Definition of new program parameters for the 788 IC Filtration Sample Processor under Configuration/Control: FILL A 2 INJECT A 2 ZERO 2 = ---***0*010*** = ---***1*000*** = ---***0*011*** 2.
2 Installation 2.4.7 Modular anion system with chemical suppression and dialysis MIC-7 It is possible to operate the 788 IC Filtration Sample Processor in combination with an anion IC system with dialysis. For this purpose, the ultra-filtration cell of the 788 IC Filtration Sample Processor should be bridged (see section 2.3.10).
2.4 Installation for full control with «IC Net» Settings in the «IC Net 2.1» program There are two ways to operate an automated Metrohm IC system with dialysis: A) For each sample, every analysis procedure contains first a dialysis step followed by the chromatographic separation. The total analysis time per sample results from summation of the single times of dialysis and separation. B) Chromatographic separation of one sample and dialysis of the next sample is done simultaneously.
2 Installation B) Separation with simultaneous dialysis of the next sample The following time program descriptions are based on chromatogram times of 20 minutes. Approx. 10 minutes before end of each separation the dialysis of the next sample is started. For this purpose, the 788 program in the system dialysis.smt contains a waiting time of 540 s in line 006 (see below). These values have to be adapted, if necessary.
2.4 Installation for full control with «IC Net» 5. Change this program for 'start-dialysis.
2 Installation 2.5 Installation with compact IC systems 2.5.1 Connection to 761 Compact IC or 790 Personal IC Compact IC systems 761 Compact IC and 790 Personal IC are remote controlled by PC software. The 788 IC Filtration Sample Processor is connected to these IC systems via its remote connection: 761 / 790 788 6.2141.110 cable Fig. 17: Interconnection with compact IC systems Tubing connections are described in section 2.10.
2.5 Installation with compact IC systems The corresponding settings in the PC software vary according to the IC system which is used: Settings in the «761 Compact IC» program A time program must be drawn up for the selected system which first produces an impulse at remote lead 3 to start sample changing on the 788 IC Filtration Sample Processor and then switches the injection valve to the "Fill" position. A further impulse is then produced to start filling the sample loop within 240 s.
2 Installation Settings in the «790 Personal IC» program A time program must be drawn up for the selected system which first produces an impulse at remote lead 3 to start sample changing on the 788 IC Filtration Sample Processor and then switches the injection valve to the "Fill" position. A further impulse is then produced to start filling the sample loop within 240 s. At the end the injection valve is switched to the "Inject" position, which also starts data acquisition.
2.6 Installation for simple remote control of the IC system 2.6 Installation for simple remote control of the IC system Using the 714 PC Board or 771 IC Compact Interface a simple interconnection of modular IC systems to a PC can be established. These devices just convert the analog signals from the 732 IC Detector to digital data for the PC. For simple control of the IC system, the 732 IC detector has to be connected to the PC additionally via its RS232 port.
2 Installation 2.6.1 IC system without suppression The 788 IC Filtration Sample Processor is connected to an IC system without suppression consisting of 732 IC Detector, 733 IC Separation Center and 709 IC Pump as shown in Fig. 18 using the 6.2141.110 cable. With this interconnection the standard methods 'PC', 'PC Seg', 'SP' and 'SP Seg' can be used (see section 4.6). 732 6.2125.090 cable 733 6.2125.060 cable 709 788 6.2141.110 cable Abb. 18: Interconnection with IC system without suppression 2.6.
2.6 Installation for simple remote control of the IC system 2.6.3 IC system with suppression with PC as "Master" The 788 IC Filtration Sample Processor is connected to an IC system with suppression consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump and either 752 Pump Unit or 753 Suppressor Module as shown in Fig. 20 using the 6.2125.120 adaptor. With this interconnection, in which the PC is the "Master", the standard methods 'PC', 'PC Seg' and 'Preconc' can be used (see section 4.6).
2 Installation 2.6.5 IC system with sample dialysis The 788 IC Filtration Sample Processor can be connected to an IC system with sample dialysis. However, this application is not planned for usage together with the ultra-filtration cell, which should be bridged. The sample processor is connected to the modular IC system consisting of 732 IC Detector, 733 IC Separation Center, 709 IC Pump, 754 Dialysis Unit and (if suppression is used) the 753 Suppressor Module as shown in Fig. 22 using the 6.2125.
2.7 Connection of devices to the RS232 interface 2.7 Connection of devices to the RS232 interface 2.7.1 General information on RS232 interface Many different instruments may be connected via the serial RS232 interface 40. 40 In addition to all Metrohm instruments that support the Metrohm remote control language (see section 5.2) any printer with serial interface (or parallel interface and parallel/serial converter) or a personal computer (PC) may be connected.
2 Installation 2.7.
2.7 Connection of devices to the RS232 interface If you connect other printers, ensure that these emulate a printer mode supported by the 788 IC Filtration Sample Processor. Please note, that some printers with a GDI-Interface do not have such printer emulation. They require a direct control by a personal computer and therefore cannot be used with a 788 IC Filtration Sample Processor. Most printers with a serial interface are connected using the 6.2125.050 cable.
2 Installation 44 788 IC Filtration Sample Processor
3.1 Prerequisites / Preparations 3 Operating tutorial In order to become acquainted with the 788 IC Filtration Sample Processor and its mode of operation, it is helpful to work through the short Operating Tutorial. The basic operating steps that are required to prepare the first sample series and run it with a given method are described here. For further explanations of the operation, please refer to section 4, which describes the functions of the individual keys and the programming in detail. 3.
3 Operating tutorial 3.2 Configuration CONFIG ENTER x The dialog language can be set in the configuration menu. Press configuration >auxiliaries x and then . >auxiliaries dialog: TOWER SELECT english x This menu item has a colon, indicating that here the parameters can be selected from a list. Press
3.3 Rack configuration RESET CLEAR 3.3 x At the end of this basic configuration the 788 IC Filtration Sample Processor must be turned off and on again or re-initialized by pressing to make the latest settings effective. x All data entered up to this point however, are retained. The same is true for any methods that may have been saved. Rack configuration x Using the keys <Ï> and <Ð> you can run the lift to the desired work position for the needle.
3 Operating tutorial >rack definitions work position 2 71 mm x In any case don’t forget to confirm the value with . >rack definitions 2 rinse position 125 mm x The next menu item 'rinse position' defines the height at which the lift must be when the needle is rinsed. As for the work position, the value here can also be entered manually or automatically accepted. For the latter, the configuration menu must be exited by pressing twice and the lift newly positioned.
3.4 Methods 3.4 Methods USER METHOD x Now open the user method menu by pressing . methods >recall method x Press to load a predefined method. >recall method method: TOWER SELECT 3.5 x Choose 'SP' with the
3 Operating tutorial parameters >start sequence x In the submenu '>start sequence' the commands that are executed at the start of a sample series are found. >start sequence 1 CTL:Rm: <Ð> INIT x The first command is a CTL command for the initialization of the remote interface. This command should be used in the start sequence of every method. Do not change anything here and press <Ð>.
3.5 "Tracing" HOLD x Press to access the LEARN mode. The blinking LEARNLED indicates that the 788 IC Filtration Sample Processor is ready to execute the command. x Now move the lift into the desired position with the <Ð> and <Ï> keys. You will notice that the current lift position is always indicated "live". During execution of the command the LEARN-LED is lit continuously. Accept the lift position that has been set by pressing and thereby exit the LEARN mode.
3 Operating tutorial >sample sequence 8 WAIT 1200 s x In this line a waiting time is defined that is used for the acquisition of the chromatogram. x The LEARN mode can also be used with the WAIT command. >sample sequence 9 NOP <Ð> x An empty line with a 'NOP'-entry (no operation) always forms the end of a sequence. x Exit the sample sequence with and go to the final sequence. x After all sample tubes have been processed, the final sequence is executed.
4.1 Fundamentals of operation 4 Operation This section provides a detailed description of the operation of the 788 IC Filtration Sample Processor using the keyboard and dialog display. The overview of the fundamentals of operation and key functions (section 4.1) is followed by a detailed description of configuration settings (section 4.2), methods (section 4.3), manual operation (section 4.4), sample racks (section 4.5), standard methods (section 4.6), and filtration principles (section 4.7). 4.
4 Operation 4.1.2 Keyboard Metrohm SC Controller ******** counter PUMPTOWER 1 CONFIG TOWER 2 PARAM DELETE LEARN USER METHOD END HOME INSERT 1/999 ready SAMPLE MOVE LIFT 7 8 9 PUMP STIR DOS 4 5 6 TOWER RESET SCAN CTRL WAIT SELECT CLEAR 1 2 3 DEF PRINT QUIT ENTER 0 . < ENDSEQ > * LEARN HOLD STOP START 6.2142.010 Most of the keys have two functions according to whether the 788 IC Filtration Sample Processor is in the normal state or in the edit mode.
4.1 Fundamentals of operation 4.1.3 Key CONFIG PARAM USER METHOD HOME Overview of key functions Normal state Edit mode Open the configuration menu x The key opens the selection menu for the configuration of the 788 IC Filtration Sample Processor . x The settings in the configuration menu remain constant until they are changed or the working memory (RAM) for the configuration settings is re-initialized.
4 Operation Key END 56 Normal state Edit mode Lift in work position x The key runs the lift into the work position. x The work position is defined separately for every sample rack in the configuration menu under '>rack definitions' (in mm from the rest position, i.e. as measured from the upper stop). Select the last line of a menu x With the key, the last line in a menu or a sequence can be accessed.
4.1 Fundamentals of operation Key Normal state Edit mode Turn rack right x The <Î> key turns the sample rack one position to the right, i.e. in the clockwise direction. The next lowest beaker position is placed under the needle. x The turning speed of the rack can be defined in the Parameter menu. x The rotation of the rack can only be carried out when the lift is at or above the shift position.
4 Operation Key RESET CLEAR Normal state Edit mode Initialization of the 788 IC Filtration Sample Processor x The key serves to initialize the IC Sample Processor. x A method in the working memory remains unchanged. The sample rack and the lift return to their initial positions in this case. Deleting parameters, setting the default values x The key sets the initial (default) value given for a parameter.
4.1 Fundamentals of operation Key Normal state Accept data, next line x The key accepts the value entered and selects the next menu line. x A modification of data or parameters must always be confirmed by , otherwise the change will not be accepted. x If a change in a parameter is not confirmed by and another menu line is accessed, the previous value will be retained. This is indicated by an acoustic signal.
4 Operation Key LIFT 9 PUMP 4 SCAN 1 60 Normal state Edit mode Positioning the lift x Raises or lowers the lift to a predefined position. These positions (work position, rinse position, shift position, special position) can be defined in the Configuration menu as rack-specific. x An absolute lift position in mm can also be given. Numerical entry ('9') or Control pump x The key is used to switch the peristaltic pump of the 788 IC Filtration Sample Processor on or off.
4.1 Fundamentals of operation Key CTRL 2 Normal state Edit mode Interface control x Controlling of external instruments via the remote and RS232 interface. This function is used for data communication with or control of connected instruments. x The first parameter stands for the selection of the interface. The second parameter defines the state of the remote output lines or data to be transmitted via RS232 interface.
4 Operation Key Normal state Edit mode x "<" serves also for shifting the chain of characters to chose from, i.e. the text cursor is shifted one place to the left for each instance (see section 4.1.6). ENDSEQ > * Initialization x The 788 IC Filtration Sample Processor is initialized with the key followed by . x A method in the working memory remains unchanged. The rack and the lift are run to the initial position.
4.1 Fundamentals of operation Key STOP LEARN HOLD Normal state Edit mode Stop process and peripheral instruments x The key terminates a method. x Any peripheral instruments that are connected (e.g. 709 IC Pump) are not automatically stopped. In '>manual stop options', a submenu of the Parameter menu, you can specify what signal or data is to be transmitted via the interface involved (remote or RS232) during manual activation of the key.
4 Operation 4.1.4 Instrument dialog The instrument dialog of the 788 IC Filtration Sample Processor is organized in the form of so-called rolling inquiries which are arranged in menu levels in a hierarchical manner and are subject to the following rules: Main menu The function keys , and of the 788 IC Filtration Sample Processor open a main menu whose thematically arranged submenus are selected by repeated pressing of this key or with <Ð>.
4.1 Fundamentals of operation Normal state KEY Kex title >Submenu 1 QUIT ENTER >Submenu 1 Inquiry 1 ENTER KEY QUIT >Submenu 1 Inquiry 2 Key title >Submenu 2 ENTER >Submenu 1 Inquiry n ENTER >Submenu 2 Inquiry 1 ENTER KEY QUIT >Submenu 2 Inquiry 2 ENTER >Submenu 2 Inquiry n Key title >Submenu n KEY Fig.
4 Operation 4.1.5 Data entry Edit line In a menu line or a sequence one or two parameters respectively can be entered. A blinking block cursor indicates where a parameter can be entered. >changer settings lift rate 1 Ű12 mm/s Ï Ï Ï menu item cursor parameter >sample sequence 7 PUMP 1.1 : Ï Í title line Í edit line Ï command 1st param. Ű150 s Í title line Í edit line Ï 2nd param. The cursor keys<Î> and <Í> can be used to switch between the parameters.
4.1 Fundamentals of operation 4.1.6 Text entry The text editor can be used when text entry is provided. Numbers can be entered directly via the keyboard. Opening the text editor PRINT . < ENDSEQ > The keys "< <" or "> >" open the text editor. With "< <" an existing character string is deleted and the text cursor is set to the left margin of the edit line. With "> >" an existing character string remains and the text cursor is set on the last character of the existing text.
4 Operation Scheme >store method method: ******** "<" or ">" blinking text cursor character chain >store method à ABCDEFGHIJ...abcd...1234...*%&/()=... method: 2x ">" blinking text cursor >store method method: à CC DEFGHIJ...abcd...1234...*%&/()=... "<" or ">" ...... >store method method: Chloride reject accept An entire text line can be entered in this way, for example, for the naming of a method. Text editing can be ended with .
4.2 Basic settings 4.2 Basic settings 4.2.1 Configuration – key CONFIG The key is used for the entry of basic settings of general applicability. If you have changed the configuration, press or switch on and off the instrument to make the entries effective.
4 Operation >auxiliaries beeper: ON ON,OFF >auxiliaries device label 8 ASCII characters >auxiliaries program 5.788.0010 read only >auxiliaries max. lift way 125 mm 0}125 mm Acoustic warning signal ON Acoustic warning signal in case of error messages OFF No warning signal Device label Freely selectable ASCII character string for characterizing the instrument (text entry, see section 4.1.6). Number of the program version Display only (no entry possibility).
4.2 Basic settings >rack definitions 2 type: M129-2 M129-2} } >rack definitions 2 work position 125 mm 0}125 mm >rack definitions 2 rinse position 125 mm 0}125 mm >rack definitions shift position 2 0 mm 0 mm >rack definitions special position 2 0 mm 0}125 mm >rack definitions >>special positions 2 Type description of the rack
4 Operation Settings for RS232 interface For further details on the RS232 interface, see section 5.2.
4.2 Basic settings 4.2.2 Locking keyboard functions Certain domains of the user dialog can be made inaccessible to the novice user by locking particular keys. For example, the inadvertent overwriting of a method or even the changing of parameters can be prevented in this way. Switch on + CONFIG The menu '>keyboard options' for the corresponding functions is opened by holding down the key while switching on the 788 IC Filtration Sample Processor.
4 Operation Submenu for locking method storage functions >keyboard options >user methods >user methods lock method recall: OFF Lock loading of methods ON,OFF >user methods lock method store: OFF Lock storage of methods ON,OFF >user methods lock method delete: OFF Lock deletion of methods ON,OFF >keyboard options lock display: OFF ON,OFF 74 Locking the display If the 788 IC Filtration Sample Processor is to be operated exclusively by an external control software (e.g.
4.3 Methods 4.3 Methods 4.3.1 Structure of a method A method consists of the following parts: x Number of samples to be processed x Process sequences (start, sample and final sequence) x Definition of the various instrument settings (changer settings, manual stop options) These method parameters are accessible by pressing the key (see section 4.3.2). With the key, methods can be stored, loaded and deleted (see section 4.3.8).
4 Operation 4.3.2 Method parameters – key PARAM The key combines the most important parameter settings for the 788 IC Filtration Sample Processor. All these settings constitute a method and may be saved as such (see section 4.3.8).
4.3 Methods parameters >start sequence Start sequence of a sample series The processing sequence entered here is executed once at the start of a sample series. This can be useful for switching on external devices, for example. In this submenu up to 99 command lines can be entered as a processing sequence (see section 4.3.3). parameters >sample sequence Processing sequence for each sample This processing sequence is executed during the processing of every sample of a series.
4 Operation parameters >manual stop Actions at manual stop The following entries define the commands or signals that are transmitted via the interfaces when the key is pressed. This enables peripheral instruments connected to be stopped automatically. >manual stop CTL Rmt: ************** 14 bit (1,0 or *) Signal output via remote interface The 3 lines 11, 12 and 13 are occupied for the swing head and are therefore ignored (see section 5.1).
4.3 Methods 4.3.4 LEARN mode LEARN HOLD When editing a method, the parameters of a command are most easily determined experimentally, i.e. by manual execution, and it is for this reason that certain commands are "teachable". The LEARN function makes the manual execution of particular changer commands possible during the editing of a sequence. The resulting parameters (for example, the lift position or the status of the input lines) can be taken over in the current command line.
4 Operation 4.3.6 Commands for sequences The following commands are programmable within a sequence. Most of them are also available in manual operation but may vary partially in their operation or exhibit a limited parameter selection (see section 4.4). SAMPLE SAMPLE 7 >start sequence 1 SAMPLE: = 1 =,+,–; 1}999 Define current sample position The SAMPLE command defines the rack position for the current sample (SAMPLE = X). This is stored as a index variable.
4.3 Methods LIFT LIFT >sample sequence 3 LIFT: 1 : rest mm 9 Position the lift work,rinse,shift, special,rest,0}125 mm Raising or lowering the lift to a defined position. Work, rinse, shift and special position are rack-specifically defined in the Configuration menu under '>rack definitions' (see section 4.2.1). The rest position is the zero position (0 mm) of the lift, i.e. the upper stop. Each lift can also be precisely positioned to the millimeter.
4 Operation Predefined bit patterns are supported which can be selected by short names (e.g. 'Ready1' or 'End2'). signifies a static "ready" line of an external instrument. 'End' stands for pulse signals, for example EOD (= end of determination). When scanning for pulse signals parallel scanning of several lines cannot be applied. 'Ready' Setting special bit patterns allows flexible control of connected instruments.
4.3 Methods More detailed information about the syntax can be found in the Instructions for Use of the instrument from which the status messages should be transmitted. CTL With the LEARN function transmitted data (= character strings) can be taken over interactively (see section 4.3.4).
4 Operation Data (= character string) can be sent to instruments connected via the serial RS232 interface. Make sure that the transmission parameters of the RS232 interface correspond to those of the connected instrument (see Configuration menu '>RS232 settings', section 4.2.1). Any letters, numbers and special characters can be chosen from the character set of the 788 IC Filtration Sample Processor. This function is suitable for instruments with Metrohm remote control language.
4.3 Methods 4.3.7 Process control START STOP LEARN HOLD RESET CLEAR QUIT With a method is started from the normal state. If there is no manual intervention or unexpected errors, the sample series is correctly processed and closed with the final sequence. The sample sequence is executed repeatedly according to the entry under 'number of samples', beginning with the sample tube that is defined as 'SAMPLE'.
4 Operation 4.3.8 User defined methods USER METHOD The key is used for the management of user defined methods, which can be stored and recalled under freely selectable names. A method comprises the parameters defined under the key. The key opens the following main menu: methods >recall method Recall method methods >store method Store method methods >delete method Delete method Repeated pressing of the key selects the submenus in turn.
4.3 Methods Deletion of methods methods >delete method >delete method method: ******** 8 ASCII characters >delete method delete ******** ? 4.3.9 Selection of the method Selection of the method which should be deleted using the
4 Operation 4.4 Manual operation 4.4.1 Turning the sample rack / Positioning the samples Using the <Í> and <Î> keys the sample rack can be turned one position to the left (in the counterclockwise direction) or right (clockwise). MOVE manual operation MOVE 1 : 8 sample Position vessel / Turn rack sample,spec.1}8, 1}999 With the MOVE command, a particular sample tube or other vessel can be placed under the needle.
4.4 Manual operation 4.4.3 Setting the sample position SAMPLE 7 manual operation SAMPLE: = 1 Define current sample position 1}999 The command serves to set the current sample position. It defines the first sample tube for the subsequent sample series. 4.4.4 Pump control PUMP 4 ******** counter PUMP+ 1/128 ready Switch pump on/off PUMP+,PUMP- The PUMP command is used to switch on/off the peristaltic pump at the 788 IC Filtration Sample Processor.
4 Operation 4.4.
4.4 Manual operation 4.4.7 Print out reports PRINT . < ******** counter Print: Print out a report 1/128 param all config param usermeth All reports in the order usermeth, config, param Configuration report Method parameter report Report of stored methods The key is used for the output of reports on an external printer or PC connected to the RS232 interface (see section 2.5). Example of "config" report 'co 788 IC Filtr. Proc. 5.788.
4 Operation Example of "param" report 'pa 788 IC Filtr. Proc. 5.788.0010 parameters method ******** number of samples: rack >start sequence >sample sequence >final sequence >changer settings rack number 0 lift rate 1 12 mm/s shift rate 20 >manual stop CTL Rmt: ************** CTL RS232: ------------ Example of "usermeth" report 'um 788 IC Filtr. Proc. user memory >methods PC PC Seg SP SP Seg 761 761 Seg An Cat AnCatSeg Preconc Dialysis remaining bytes ------------ 92 5.788.
4.5 Sample racks 4.5 Sample racks 4.5.1 Standard rack (6.2041.430) Metrohm delivers the following standard sample rack for the 788 IC Filtration Sample Processor: Type M129-2 Number of vessels 127 2 Type of vessel Predef. code Predef. rack no. PP Sample tube (6.2743.050; 11 mL) PE Bottle (6.1608.080; 300 mL) 010001 2 If desired, other user-defined racks can be delivered and defined in the instrument via PC software. Irregular arrangements of vessel positions are also possible. 4.5.
4 Operation When a sample series is started the 788 IC Filtration Sample Processor first runs the rack automatically into the normal starting position so that it is always ensured that the vessel positions correspond with the internal position table of the current rack. The rack code is used for automatic rack recognition. A code can be assigned only once. The standard rack supplied by Metrohm already have a predefined code.
4.5 Sample racks The Code is used for automatic rack recognition. Make sure that this 6place binary code in the rack configuration agrees with the actual inserted magnet code on the rack. Rack codes can be changed at any time. They must however, only be assigned to one rack. The assignment of standard predefined codes of standard racks provided by Metrohm should be avoided. There is one code which can be used for this purpose. It is normally used for special racks: 110000.
4 Operation 4.5.4 Special beakers Special beakers are reserved positions in a sample rack. 0 to 8 special beakers can be defined per rack. They can be placed under the needle during method processing for particular operations without interrupting or hindering the sample series run. Special beakers can be used in a sample sequence for rinsing the needle. Special beakers are placed under the needle with 'MOVE 1 : spec.1'.
4.6 Standard methods 4.6 Standard methods The following pages contain listings of standard user methods included with the instrument with explanations of the important commands. They are required if the full control of the 788 IC Filtration Sample Processor is not done by the PC directly. Therefore, the standard methods are required for the combination of the 788 IC Filtration Sample Processor with the following Metrohm IC systems: x 761 Compact IC or 790 Personal IC (see section. 2.6).
4 Operation All methods with the annex 'Seg' only differ by one additional step: PUMP 1.1 : 5 s This command is processed before the lift of the 788 IC Filtration Sample Processor is placed to working position. Thereby, a small segment of air is introduced into the tubing between two samples. Therefore, these methods should not be used together with the ultra-filtration cell. In the following methods, this command is written in brackets without a line number.
4.6 Standard methods 4.6.1 Method "PC" and "PC Seg" Application Processing a queue with the PC program with the PC as "Master". Interconnection See section 2.6.1 (without suppression) and section 2.6.3 (with suppression) Program of 788 IC Filtration Sample Processor 788 IC Filtr. Proc. parameters method PC number of samples: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump1 ? 3 MOVE 1 : sample ( PUMP 1.1 : 5 4 LIFT: 1 : work 5 CTL:Rm: FILL A 1 6 PUMP 1.
4 Operation 4.6.2 Method "SP" and "SP Seg" Application Processing a queue with the PC program «IC Metrodata» with the 788 IC Filtration Sample Processor as "Master". Interconnection see section. 2.6.1 (without suppression) and section 2.6.2 (with suppression) Program of 788 IC Filtration Sample Processor 788 IC Filtr. Proc. parameters method SP number of samples: rack >start sequence 1 CTL:Rm: INIT 2 CTL:Rm: PUMP 752 ON >sample sequence 1 SCN:Rm : Pump1 ? 2 MOVE 1 : sample ( PUMP 1.
4.6 Standard methods 4.6.3 Method "761" and "761 Seg" Application Processing a queue of the PC program «761 PC Software» or «790 PC Software» with compact IC systems and the PC as "Master". Interconnection See section 2.5.1. Program of 788 IC Filtration Sample Processor 788 IC Filtr. Proc. parameters method 761 number of samples: rack >start sequence 1 CTL:Rm: INIT >sample sequence 1 SCN:Rm : Wait1 2 MOVE 1 : sample ( PUMP 1.1 : 5 3 LIFT: 1 : work 4 SCN:Rm : Wait1 5 PUMP 1.
4 Operation 4.6.4 Method "An Cat" and "AnCatSeg" Application Simultaneous determination of anions and cations. Interconnection Siehe section 2.6.4. Program of 788 IC Filtration Sample Processor 788 IC Filtr. Proc. parameters method An Cat number of samples: rack >start sequence 1 CTL:Rm: INIT 2 CTL:Rm: PUMP 752 ON >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump* ? 3 MOVE 1 : sample ( PUMP 1.1 : 5 4 LIFT: 1 : work 5 CTL:Rm: FILL A 1 6 CTL:Rm: STEP MSM 753 7 PUMP 1.
4.6 Standard methods Settings in the «IC Metrodata» program 1. Definition of anion method for channel 1 (analysis time, start parameters for IC instruments 732, 733 and 709, etc.), additional association of this method with the following time program for the 732/1 IC Detector: Method / Equipment / Setup / Program / Text: 0.0 Puls_788_set Set remote line 3 at 732/1 to 1 (s. below) 0.1 Puls_788_reset Set remote line 3 at 732/1 to 0 (s. below) Program end 0.
4 Operation 4.6.5 Method "Preconc" Application Preconcentration of samples with very low concentrations in an preconcentration column (trace analysis). This method is not planned for usage together with the ultra-filtration cell. However, if ultra-filtration is done, the preconcentration time in line 8 has to be increased to maintain a sufficient filtering time, but without complete consuming of the sample solution. Interconnection Siehe section. 2.6.3.
4.6 Standard methods Program of 788 IC Filtration Sample Processor 788 IC Filtr. Proc. parameters method Preconc number of samples: rack >start sequence 1 CTL:Rm: INIT 2 CTL:Rm: INJECT A 1 3 CTL:Rm: PUMP 752 ON >sample sequence 1 SCN:Rm : Wait1 2 SCN:Rm : Pump1 ? 3 MOVE 1 : sample 4 LIFT: 1 : work 5 PUMP 1.1 : ON 6 WAIT 60 7 CTL:Rm: FILL A 1 8 WAIT 120 5.788.0010 mm s s s 9 PUMP 1.
4 Operation 4.6.6 Method "Dialysis" Application IC determinations with automatic sample dialysis. This method is not planned for usage together with the ultra-filtration cell, which should be bridged. The present method contains a simultaneous separation of one sample and the dialysis of the following sample within the sample sequence. The start sequence just starts the dialysis of the first sample while the final sequence only starts the determination of the final sample. Interconnection See section 2.6.
4.6 Standard methods Settings in the «IC Metrodata» program Definition of IC method (analysis time, start parameters for IC instruments 732, 733 and 709, etc.), additional association of this method with the following time program for the 732 IC Detector: Method / Equipment / Setup / Program / Text: 10.0 Puls_788_set Set remote line 3 at 732/1 to 1 (s. below) 10.1 Puls_788_reset Set remote line 3 at 732/1 to 0 (s. below) Program end 10.
4 Operation 4.7 Filtration 4.7.1 Selecting suitable sample types Due to the small pore size of the filtration membrane, each filtration step may contribute to possible clogging of the membrane. In the following table some sample types are described, which have been processed by the 788 IC Filtration Sample Processor. Filtration was performed using the 6.2714.020 filtration membrane (0.15 µm) or a second filtration membrane (0.2 µm).
4.7 Filtration Finally, the sample matrix and the specifications of the analysis method determine the time for changing the filtration membrane. From our experience, suspensions cause membrane clogging earlier than bigger particles in the sample matrix. The latter should be rinsed across the membrane surface. An instruction for changing the filtration membrane is given in section 6.3.4. 4.7.
4 Operation 110 788 IC Filtration Sample Processor
5.1 Remote interface 5 Interfaces 5.1 Remote interface Peripheral instruments connected such as 732 IC Detector, 709 IC Pump, 752 Pump Unit, etc. can be controlled via the remote interface (25-pin socket). 14 lines (Output 0 – 13) are available for the emission signals. Lines 11 – 13 are used for swing head control. For receiving signals (e.g. the "ready" signal of the 732 IC Detector) 8 lines (Input 0 – 7) are provided. Line 7 is used for the swing head. 5.1.
5 Interfaces For the 788 IC Filtration Sample Processor, the output lines 11–13 and the input line 7 are occupied for the control of the swing head. These four lines are not continued in the plug and ignored, when further instruments are connected via the remote cable (see section 2.4.2). Various remote cables are available to use the specific functions of the individual instruments of the various Metrohm model lines (see section 2.4.2).
5.1 Remote interface Input lines The 8 input lines of the remote socket are queried during method processing with the "Scan" command (SCN). Method processing is interrupted until the predefined bit pattern compares to the effective state of the input lines (for example, the status of the ready line of the 732 IC Detector). An 8-place bit pattern must be set for this. Every bit is assigned to an input line. If there is correspondence, method processing will continue with the next command line.
5 Interfaces CTL commands Parameter Bit pattern Function Signal INIT 00000000000000 initializes the remote interface static INIT 732 ***0000*000**0 initializes the remote lines at 732/1 and 732/2 static PROG R/S 1 ***000*******1 starts/stops time program at 732/1 pulse (200 ms) PROG R/S 2 ******0*100*** starts/stops time program at 732/2 pulse (200 ms) PUMP R/S 1 ***001*******0 starts/stops 709/1 IC Pump pulse (200 ms) FILL A 1 ***010*******0 switches valve A at 733/1 to "Fill" pu
5.2 RS232 interface 5.2 RS232 interface 5.2.1 General rules for remote control The 788 IC Filtration Sample Processor is equipped with the comprehensive Metrohm remote control language, which allows full control over the instrument via an RS232 interface, i.e. the 788 IC Filtration Sample Processor can receive data from an external device or send data to an external device. The 788 IC Filtration Sample Processor sends 2uCR and LF as the terminator of a requested data block.
5 Interfaces 5.2.2 Call-up of objects A section from the object tree is shown below: Node 3 Language Node 2 Node 1 Prog Aux RSset Config Mode Node 0 & Root The following rules apply to the call-up of objects: Rules Examples The root of the tree is designated by ‘&’. For the call-up of an object, the nodes (levels) of the tree are marked by a point (.). For the call-up of the objects, as many characters as necessary to allow unambiguous allocation of the object suffice.
5.2 RS232 interface 5.2.3 Trigger Triggers initiate an action at the 788 IC Filtration Sample Processor , e.g. sequence start or data transmission. Triggers are marked by the introducer '$'. The following triggers are possible: $G Go Starts processes, e.g. starting the mode run or setting the RS232 interface parameters $S Stop Stops processes $Q Query Used to request all information from the current node in the tree forwards up to and including the values $Q.
5 Interfaces 5.2.4 Status messages To ensure appropriate control of an external control unit is possible, it must also be possible to request status conditions which provide information on the status of the 788 IC Filtration Sample Processor. The output of a status message is triggered by the trigger '$D'. Status messages consist of the global status, the detailed status and eventual error messages.
5.2 RS232 interface 5.2.5 Error messages Error messages ‘EXXX’ are appended to the status message and separated from this by a ‘;’. Error Meaning Exit/Corrective action E1 Incorrect program checksum Inform Metrohm service. E2 RAM read/write error Inform Metrohm service. E3 RAM lost data . If instrument adjustment is necessary, inform Metrohm service. E4 Timer interrupt for multi-tasking missing Inform Metrohm service. E5 RS232 module test error Inform Metrohm service.
5 Interfaces 5.2.6 Remote control commands The remote control tree can be divided into the following main branches: & Ō Mode Ō Config Ō Info Ō Setup Ō UserMeth Ō Assembly Ŋ Diagnose Root Method parameters Instrument configuration Instrument information Settings of operating mode User-defined methods Control of assemblies Diagnostic tests The following table lists all relevant objects of the remote control tree for the 788 IC Filtration Sample Processor.
5.2 RS232 interface Object Ň Ō.FinalSeq Ň Ō.1 Ň Ň Ō.Cmd Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ŋ.* Ň : Ň * Ň Ň Ň Ň Ň Ŋ.100 Ň Ō.Changer Ň Ō.RackNo Ň Ō.L1Rate Ň Ŋ.ShRate Ň Ŋ.ManStop Ō.RemCtl Ŋ.RSCtl Meaning Entry range/Selection Final sequence Line number of the command – – Command (see Start sequence) NOP, MOVE, LIFT, SAMPLE, PUMP, SCAN, CTRL, WAIT, ENDSEQ Parameter for command (see Start sequence) Sequence end .MOVE}, .LIFT}, .SAMPLE}, .PUMP}, .SCAN}, .CTRL}, .WAIT}, .
5 Interfaces Object Ň Ň 1Off Ň Ō.R1O 1O 2Off Ň Ō.R2O 2O Ň Ō.Num Ň Ō.1 Ň Ň Ŋ.Value Ň : Ň Ŋ.n Ň Ŋ.Value Ň Ŋ.RSset Ň Ō.Baud Ň Ō.DataBit Ō.StopBit Ō.Parity Ň.Handsh Ň Ŋ.CharSet Meaning Entry range/Selection Offset in 1/10-ang. degrees for positions in row 1 Offset in 1/10-ang. degrees for positions in row 2 Number of positions (n) Position 1 Position in 1/10-angular degrees 0}3599 0}3599 1}200 – 0}3599 Position n Position in 1/10-angular degrees – 0}3599 RS232 interface $G effects all the RS settings.
5.2 RS232 interface Object Ň Ō.Outputs Ň Ŋ.Status Ň Ň Ň Ň Ň Ō.Display Ň Ō.L1 Ň Ŋ.L2 Ň Ŋ.Counter Ō.Sample Ŋ.Maximum Meaning Entry range/Selection Remote outputs Status of the 14 output lines (Output 0}13; 1 = on, read only low, active; 0 = off, high, inactive) in byte form 13 Status ¦2 n n 0 Display Display in LCD line 1 Display in LCD line 2 – read only read only Sample counter Current sample position Number of samples to be worked off – read only read only &Setup &Setup Ň Ō.
5 Interfaces Object Ň Ň Ň Ō.UserMeth Ň Ň Ō.Recall Ň Ň Ō.Store Ň Ň Ŋ.Delete Ň Ŋ.Display Ň Ō.AutoInfo Ň Ň Ň Ň Ň Ō.Status Ň Ō.P Ň Ō.Ch Ň Ň Ō.G Ň Ň Ō.R Ň Ň Ō.S Ň Ň Ō.H Ň Ň Ō.C Ň Ň Ō.B Ň Ň Ō.F Ň Ň Ō.OM Ň Ň Ŋ.CM Ň Ŋ.Error Ň Ō.PowerOn Ň Ō.Initialize Ň Ŋ.Select Ň Ň Ō.RamInit Ň Ň Ň Ŋ.InstrNo Ŋ.
5.2 RS232 interface Object Meaning Entry range/Selection &Assembly &Assembly Ň Ō.Sample Ň Ō.Func Ň Ŋ.Value Ň Ō.Move Ň Ō.Target Ň Ŋ.Position Ň Ň Ō.Lift Ň Ō.Station Ň Ŋ.Way Ň Ň Ō.Pump Ň Ō.Address Ň Ŋ.Value Ň Ō.Scan Ň Ō.Address Ň Ŋ.Pattern Ň Ň Ň Ň Ň Ň Ō.Ctrl Ň Ō.Address Ň Ŋ.Pattern Ň Ň Ň Ň Ň Ň Ň Ň Ō.Wait Ň Ŋ.Time Ň Ŋ.
5 Interfaces Object Meaning Entry range/Selection &Diagnose &Diagnose Ň Ō.Init Ň Ŋ.Select Ň Ň Ō.RamTest Ň Ō.LcdTest Ň Ō.ContrastTest Ň Ō.KeyTest Ň Ō.IoTest Ň Ō.RsTest Ň Ō.EbusTest Ň Ō.BeeperTest Ň Ō.RackcodeTest Ň Ō.FunctionTest Ň Ō.SimulateKey Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ň Ō.InstrNo Ň Ň Ŋ.PowerOn 126 Diagnosis (see section 6.
5.2 RS232 interface 5.2.7 Data transmission protocol The RS232 interface of the 788 IC Filtration Sample Processor is configured as DTE (Data Terminal Equipment) with the following technical specifications: x Standard Data interface in accordance with EIA standard RS 232C (DIN 66020, page 1), transmission parameters can be set under 'configuration/RS232 settings', see section 4.2.1. x Control characters CR LF XON XOFF x Max.
5 Interfaces 5.2.8 Handshake No Handshake (none) The 788 IC Filtration Sample Processor neither checks handshake inputs (CTS, DSR, DCD) nor sets handshake outputs (DTR, RTS). Reduced hardware handshake (HWs) 788 IC Filtration Sample Processor as receiver: 788 External device DTR DTR LF RxD RxD Time 788 IC Filtration Sample Processor as sender: 788 External device RTS RTS CTS CTS LF TxD TxD Time The data flow can be interrupted by deactivating the CTS line.
5.2 RS232 interface 788 IC Filtration Sample Processor as sender: 788 External device RTS RTS DSR DSR DCD DCD CTS CTS LF TxD TxD Time The data flow can be interrupted by deactivation of the CTS line. Software handshake with character stop (SWchar) Handshake inputs are (CTS, DSR, DCD) are not checked at the 788 IC Filtration Sample Processor, handshake outputs (DTR, RTS) are set. As soon as an LF is recognized, the 788 IC Filtration Sample Processor sends XOFF.
5 Interfaces 788 IC Filtration Sample Processor as sender: 788 External device XOFF XON Data output RxD LF TxD max. 4 char. Data output disabled Data input Data output enabled Time Software handshake with line stop (SWline) Handshake inputs are (CTS, DSR, DCD) are not checked at the 788 IC Filtration Sample Processor, handshake outputs (DTR, RTS) are set. The 788 IC Filtration Sample Processor has an input buffer which can accept a string of up to 80 characters + CR LF.
5.2 RS232 interface 5.2.9 Pin assignment RS232C interface external Transmitted Data (TxD) If no data transmission takes place, the line is maintained in the ”ON” condition. Data are sent only when CTS and DSR are in the ”ON” condition and DCD is in the ”OFF” condition. Pin 2 Received Data (RxD) Data are received only when DCD is ”ON”. Pin 3 Transmitted Data Received Data Request to Send (RTS) ON condition: 788 IC Filtration Sample Processor is ready to send data.
5 Interfaces 5.2.10 RS232 error rectification Problem Questions for corrective action No characters can be received on an attached printer. Are the devices switched on and the connecting cables plugged in properly? Is the printer set to ”on-line”? Do the baud rate, data bits and parity for the two devices have the same setting? Is the handshake set properly? If everything appears ok, try to print out a report by pressing the key.
6.1 Error messages 6 Appendix 6.1 Error messages When an error occurs the execution of the active command is interrupted and an error message is displayed (display blinks). This must be acknowledged with the key. If an error occurs during processing of a sample series, the 788 IC Filtration Sample Processor will then be switched into the "HOLD" state.
6 Appendix 134 Error message Cause * changer low power The power supply cannot deliver enough power for the simultaneous operation of all components currently in use (pump, lift). * RS232 error The transmission parameters of the RS232 interface do not agree with those of the receiving instrument. * check rack/swing head Invalid configuration of sample rack or swing head. * invalid position The sample position selected is defined as a special beaker or the special beaker chosen is not defined.
6.2 Technical data 6.2 Technical data Lift Lift path Load Stroke speed ca. 125 mm ca. 30 N adjustable, 3}12 mm/s Turntable Turning speed adjustable, 3}20 angular degrees/s Pump Pump type 2-channel peristaltic pump with rotational speed of 20 /min Pump capacity (with water, without counterpressure; depends on contact pressure ) with 6.1826.010 pump tubing: typ. 1.5}1.7 mL/min with 6.1826.030 pump tubing: typ. 0.5}0.6 mL/min with 6.1826.070 pump tubing: typ. 1.8}2.0 mL/min Pressure max. 1.5 bar (0.
6 Appendix Interfaces Remote interface specifications, see section 5.1 RS232 interface specifications, see section 5.2 Safety specifications Construction / Testing According to IEC 1010 / EN 61010 / UL 3101-1, protection class 1 Safety directions The Instructions for Use include information and warnings which must be heeded by the user to assure safe operation of the instrument.
6.3 Maintenance and servicing 6.3 Maintenance and servicing 6.3.1 Maintenance by Metrohm service Maintenance of the 788 IC Filtration Sample Processor is best done as part of an annual service performed by specialists from the Metrohm company. If work is frequently performed with caustic and corrosive chemicals, it may be necessary to shorten the interval between servicing.
6 Appendix 6.3.3 Replacing the pump tubing Pump tubings are consumable material with a limited lifetime and should be replaced at regular intervals (approx. every 2 weeks under continuous use). The working life of pump tubing depends to a considerable extent on the contact pressure. This is why the contact pressure must be correctly set as described in section 2.3.9.
6.3 Maintenance and servicing 6.3.4 Replacing the filtration membrane A good condition of the filtration membrane is a prerequisite for a constant quality of the analysis results. Therefore, the membrane has to be replaced regularly. Please consider section 4.7 for evaluating the membrane type and time of exchange. Replace the filtration membrane as follows, see also Fig. 9 in section 2.3.8.
6 Appendix Do not connect the dialysis cell with anything other than the 6.2744.000 PVDF compression fittings supplied. If 6.2744.010 PEEK compression fittings are used, stress cracks may appear in the ultra-filtration cell ! 4 Close ultra-filtration cell x Insert sealing ring in the appropriate recess in top part 43. 43 x Place top part on bottom part 45 so that both parts lie flush. x Using the 6.2621.070 Allen key, screw the 5 screws 46 right in and tighten up.
6.4 Diagnosis 6.4 Diagnosis 6.4.1 General Information The 788 IC Filtration Sample Processor is a very precise and reliable control instrument. Thanks to its rugged construction, its functions are rarely influenced by mechanical or electrical affects. Although an occasional fault in the instrument can not be excluded completely, it is certainly much more likely that malfunctions are caused by wrong operation or handling or by improper connections and operation with non-Metrohm instruments.
6 Appendix Required Instruments: Only required, if the RS232 or remote interface is to be checked: x 3.496.8550 Test plug (on the "Remote" plug) x 3.496.8480 Test plug (on the "RS 232" plug) 6.4.2 Preparing the instrument x Disconnect power plug. x Remove cables to the "RS232" and "Remote" interfaces. x Plug in power plug and immediately press <9> and keep it depressed until the start test pattern disappears.
6.4 Diagnosis 6.4.3 Working memory (RAM) This diagnosis step completes a non-destructive test over the entire range of the RAM contents (working memory). x Prepare instrument for diagnosis (see section 6.4.2). x If necessary, press <Ð> several times until diagnosis >RAM test x If no errors are found, this appears on the display: >RAM test RAM test ok x diagnosis >display test 6.4.4 Display With this diagnosis step, the functionality of the LED’s and the display is tested.
6 Appendix diagnosis >display contrast test x After pressing the key the following display appears whereby the contrast of the display continuously varies between bright and dark. >display contrast test 788 IC Filtr. Processor x The test can be exited with the or key. diagnosis >key test 6.4.5 Keyboard This diagnosis step allows all the keys on the keyboard to be tested for their functionality. x Prepare instrument for diagnosis (see section 6.4.2).
6.4 Diagnosis Key code table Code Key 0 Key 16 <7 / SAMPLE> 1 17 <4 / PUMP> 2 18 <1 / SCAN> 3 19 <0> 4 20 5 21 <Î> 6 22 23 7 6.4.6 Code 8 <9 / LIFT> 24 <Ï> 9 <6> 25 <Ð> 10 <3 / WAIT> 26
6 Appendix 6.4.7 RS232 interface This diagnosis step tests the functionality of the serial interface. x Prepare instrument for diagnosis (see section 6.4.2). x If necessary, press <Ð> several times until diagnosis >RS232 test x >RS232 test RS232 test connector ? x Insert the 3.496.8480 test plug into the RS232 interface without turning off the instrument. x The test runs automatically.
6.4 Diagnosis 6.4.9 Beeper x Prepare instrument for diagnosis (see section 6.4.2). x If necessary, press <Ð> several times until diagnosis >beeper test x The beeper is turned on and off in an endless loop. >beeper test x The test can be exited by pressing the or keys. diagnosis >rack code test 6.4.10 Rack code recognition This diagnosis step checks the functionality of the sensors with regard to their ability to automatically recognize the attached rack.
6 Appendix x The test reads the code continuously and displays it immediately. For representation on the screen a 6-place bit pattern is provided (code ??????). The first position is for magnet no. 1, the second position for magnet no. 2, etc. If a magnet is recognized, a “1” is written into the corresponding position, otherwise a “0” is assigned. x Position all racks which are used one after the other and compare the notes made before the test with the information on the display.
6.5 Initialize data memory x Prepare instrument for diagnostic test (see section 6.4.2). x If need be, press <9> key repeatedly until diagnosis >RAM initialization x Press key to open the following diagnostic menu: >RAM initialization select: param Set method parameters with default values. >RAM initialization select: config Set config parameters with default values. >RAM initialization select: setup Set setup parameters with default values.
6 Appendix 6.6 Validation / GLP GLP (Good Laboratory Practice) requirements include the periodic check of analytical instruments for reproducibility and accuracy using Standard Operating Procedures (SOP). Although the instrument in question is not a measuring instrument, it is recommended to include the 788 IC Filtration Sample Processor in the validation of an analytical system as a part of it.
6.7 Warranty and conformity 6.7 Warranty and conformity 6.7.1 Warranty The warranty on our products is limited to defects that are traceable to material, construction or manufacturing error which occur within 12 months from the day of delivery. In this case, the defects will be rectified in our workshops free of charge. Transport costs are to be paid by the customer. For day and night operation, the warranty is limited to 6 months.
6 Appendix 6.7.2 EU Declaration of conformity EU Declaration of Conformity The METROHM AG company, Herisau, Switzerland hereby certifies that the instrument: 788 IC Filtration Sample Processor meets the requirements of EC Directives 89/336/EWG and 73/23/EWG.
6.7 Warranty and conformity 6.7.3 Certificate of conformity and system validation Certificate of Conformity and System Validation This is to certify the conformity to the standard specifications for electrical appliances and accessories, as well as to the standard specifications for security and to system validation issued by the manufacturing company. Name of commodity: 788 IC Filtration Sample Processor Name of manufacturer: Metrohm Ltd.
6 Appendix 6.8 Standard equipment Subject to changes ! In figures, all dimensions are given in mm. The 2.788.0010 IC Filtration Sample Processor includes the following parts: Quant. Order No. Description 2 6.1608.080 PE Bottle 300 mL Bottle for special beaker positions 1 (128) and 2 (129) 128 67 PTFE Capillary outer diameter: 1/16 '' 1 1 1 2 6.1803.050 6.1803.060 6.1803.070 6.1803.080 X (i.d.) [mm] 0.50 0.97 0.97 0.
6.8 Standard equipment Quant. Order No. Description 1 6.2041.430 Sample rack (M129-2) For 127 sample tubes 6.2743.050 (11 mL) and 2 PE bottles 6.1608.080 (300 mL) 1 6.2122.0X0 Mains cable To customer's specifications: Cable socket Cable connector Type IEC 320/C 13 Type IEC 320/C 13 Type CEE (22), V Type SEV 12 (CH})............................ 6.2122.020 Type CEE (7), VII (D})........................ 6.2122.040 Type NEMA 5-15 (USA})................... 6.2122.070 1 6.2141.
6 Appendix Quant. Order No. Description 1 6.2743.050 PP Sample tube (11 mL) For 6.2041.430 sample rack; set of 2000 2 6.2743.070 PP Cap For sealing the 6.2743.050 sample tubes; set of 2000 1 6.2744.000 PVDF Compression fitting For connecting 6.1803.0X0 PTFE capillaries to the filtration cell, set of 5 1 6.2744.010 21 PEEK Compression fitting For connecting 6.1831.0X0 PEEK capillaries or 6.1803.0X0 PTFE capillaries, set of 5 26 1 156 6.2744.030 PEEK Coupling Connection between 6.2744.
6.9 Optional accessories 6.9 Optional accessories Order No. Description 6.1831.040 PEEK Capillary Length = 15 cm For the connection of two injection valves at the 733 IC Separation Center 0.5 1.59 6.2125.090 Connection cable Connection cable 788 IC Filtration Sample Processor – 6.2125.120 Adaptor 6.2125.120 Adaptor for remote connection With 5 connections for remote cables 6.2141.120 Connection cable Connection cable 788 IC Filtr. Sample Proc. – 732/2 IC Detector – 709/2 IC Pump 6.2743.
6 Appendix 158 788 IC Filtration Sample Processor
6.10 Index 6.10 Index # 714 PC Board .......................................................37 754 Dialysis Unit ...................................................40 761 Compact IC Connection......................................................34 PC software settings.......................................35 762 IC Interface.....................................................23 771 IC Compact Interface.....................................37 790 Personal IC Connection.....................................
6 Anhang Display Contrast ..........................................................69 Description......................................................53 Locking ...........................................................74 Representation .................................................6 Test ...............................................................143 display contrast..........................................69 Documentation .......................................................7 E Earthing .....
6.10 Index LCD-Display........................................................135 Leaks ...................................................................8,9 LEARN mode ...................................................50,79 LIFT ................................................................81,88 Lift 3 Figure ................................................................3 Manual operation ............................................88 Positioning .....................................................
6 Anhang PEEK capillary 6.1831.040 Interconnection of two injection valves...........22 Ordering designation....................................157 PEEK compression fitting 5 Connect PEEK capillary..................................20 Figure...........................................................3,14 Needle installation ..........................................13 PEEK compression fitting 6 Figure.................................................3,14,19,20 Ordering designation..............................
6.10 Index Error messages.............................................119 Handshake....................................................128 Pin assignment .............................................131 Remote control .............................................115 Remote control commands ..........................120 RS232 error rectification ...............................132 Scanning .........................................................82 Sending data..............................................
6 Anhang V Validation ............................................................150 W WAIT.....................................................................84 Waiting time ..........................................................84 Warning...................................................................6 Warning signal ......................................................70 Warranty..............................................................151 Waste container...................................
6.10 Index Keys Menus < < >...............................................................67 < > >...............................................................67 .............................................58,64,67,85 ...........................................46,55,69,73 ..............................................61,83,90,114 .....................................................57,78 <Ð> ..........................................................45,56,88 ......
