Owner manual

4 Operation
761 Compact IC
118
Notations
R Stands for response value, either area or height, de-
pending on setting selected in the
Calibration graphs
window.
V Sample
Volume injected.
D
Dilution coefficient, shows number of times to which
the initial solution is dissolved before injection.
V' = V / D
Adjusted volume of injected sample. A correction is
made for the dilution coefficient.
C Concentration of the component in the initial solution
(before dilution).
Q = C
• V' Quantity of component, used for calibration curve
construction.
t
Retention time. Time needed by the mobile phase to
flow through the separation system.
t
0
Void time. Dead time needed by the mobile phase to
flow through the separation system.
t' = t – t
0
Corrected retention time, called also net retention time.
L Column length.
v = L / t
0
Linear Flow rate.
W(R) = k
2
R
2
+ k
1
R + k
0
Calibration function (component quantity W vs. de-
tector response R). In the case of the most common
linear calibration curve Q = W(R) = k
1
R it comes
through the origin. The concentration of the compo-
nent in the analyzed mixture is calculated by the for-
mula C = W(R) / V'.
RSD(Q, R) Procedure, used for computation of regression coef-
ficients (k
0
, k
1
and k
2
) of the calibration function W(R)
using RSD (Residual Standard Deviation). The proce-
dure gets input as a set of calibration points (quantity
Q vs. response R) and outputs the calibration func-
tion W(R) used for prediction of the component
quantity Q
i
= W(R
i
).
Subscript values used:
j Stands for j-th calibration level run.
s Stands for standard component.
i Stands for component number.