User guide

3.3 The dialysis procedure
754 Dialysis Unit
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3.3 The dialysis procedure
3.3.1 Dialysis flow chart
Every sample dialysed with the 754 Dialysis Unit comprises the follow-
ing four steps:
1
Rinsing
Valve A: FILL
Valve B: INJECT
Sample channel, ac-
ceptor channel and
sample loop are rinsed
(rinsing time: approx. 2
min).
A B
Fill Inject
2
Dialysis with «Stopped Flow»
Valve A: FILL
Valve B: FILL
The sample is dialysed
with resting acceptor
solution (dialysis time:
approx. 10 min).
A B
Fill Fill
3
Transfer to sample loop
Valve A: FILL
Valve B: INJECT
The acceptor solution
enriched with ions from
the sample is trans-
ferred to the sample
loop (transfer time:
approx. 0.4 to 0.6 min).
A B
Fill Inject
4
Injection
Valve A: INJECT
Valve B: INJECT
The acceptor solution
enriched with ions from
the sample is injected
into the IC system.
A B
Inject Inject
Eluent
Column
Waste
Sample
Acceptor
Eluent
Column
Waste
Sample
Acceptor
Eluent
Column
Waste
Sample
Acceptor
Eluent
Column
Waste
Sample
Acceptor