Manual

2 Installation

750 Autosampler

2.2.4 Tubing connection

For transferring the sample from the 750 Autosampler to the injection

valve of the 733 IC Separation Center the following tubing connections

must be made:

Tubing connection 750 – injection valve

• At the 733 IC Separation Center, loosen the rotary nipple

screwed onto the interior side of connection 2222 or 2828.

• Take PTFE suction tubing 8888 (see Fig. 16 and Fig. 17 of the

732/733 Instructions for Use) completely out of connection 2222

or 2828 and unscrew from connection "1" of injection valve 6868.

• Cut transfer tubing 88 (6.1803.000 PTFE capillary tubing of 750

accessories) to the shortest possible length between the

needle of the 750 Autosampler and injection valve 6868 of the

733 IC Separation Center.

• Remove the cover from transfer needle 55 of the 750 Auto-

sampler and screw one end of transfer tubing 88 to this con-

nection with the aid of a 6.2744.010 PEEK compression fit-

ting.

• Remove holder stopper 77 from holder 66 and pull 6.1803.000

PTFE capillary tubing so far through the opening of holder

stopper 77 that the tubing length between needle connection

and the holder stopper is about 30 cm. Then press holder

stopper 77 fully back into holder 66.

• Pull the other end of the transfer tubing 88 through the opening

2222 or 2828 of the 733 IC Separation Center and screw onto

connection "1" of injection valve 6868 using a 6.2744.010 PEEK

compression fitting.

• Retighten rotary nipple on the interior side of connection 2222

or 2828 to fix the capillary tubing.

Tubing connection injection valve – waste

• Insert 6.2744.020 coupling (from 733 accessories) into con-

nection 2121 or 2727 of the 733 IC Separation Center.

• Screw PTFE suction tubing 8888 onto the 6.2744.020 coupling

attached to connection 2121 or 2727 and lead it into the waste

container.

In the case of the 2.733.0X20 IC Separation Center with two injection

valves, it is possible to fill both sample loops from the same 750

Autosampler. For this, connection "1" of valve A (outlet of the sample

loop) must be connected to connection "2" of valve B (inlet of the

sample loop) using a 6.1803.000 PTFE Capillary tubing cut to the

appropriate length.