User guide

2.8 Separating columns and suppressor module

732 IC Detector / 733 IC Separation Center

2.8.4 One-channel system without suppressor module

With the one-channel system without suppressor module, the IC sepa-

rating column is installed in the 2.733.0010 IC Separation Center as

follows (see Fig. 16):

1 Connect column to injector

• Remove end caps from column 7676.

• without precolumn:

Screw inlet end of separating column 7676 (note flow direction)

to column connection capillary 6767 mounted on the injector.

• with precolumn in twin cartridge holder:

Screw inlet end of separating column 7676 (note flow direction)

to outlet capillary 7272 (see Fig. 15).

• with precolumn in cartridge head:

Install separating column 7676 (note flow direction) in the car-

tridge head (see Fig. 15) as described in section 2.7.3.

2 Rinse column

• Place a beaker beneath the column outlet.

• Switch on 709 IC Pump and rinse the column for ca. 10 min

with eluent.

• Switch off IC Pump.

3 Connect column to detector block

• Screw outlet end of separating column 7676 to the inlet capillary

8282 permanently mounted on the detector block.

4 Fix column

• Insert one or two column holders 8484 (6.2027.030, 6.2027.040

or 6.2027.050) in the mounting rails 8383 and fasten separating

column in the column holder.

2.8.5 Two-channel system without suppressor module

With the two-channel system without suppressor module (2.733.0X20

IC Separation Center), the first IC separating column is connected on

the left side to injection valve A and detector block A as with the one-

channel system (see section 2.8.4 and Fig. 16). The second column is

connected on the right side to injection valve B and detector block B in

an analogous manner.