User guide

2 Installation

732 IC Detector / 733 IC Separation Center

2.8 Separating columns and suppressor module

2.8.1 General information on separating columns

New IC separating columns are normally filled with solution and

sealed at both ends. Before the column is installed in the system, it

must be ensured that this solution is freely miscible with the eluent

used (check manufacturer's specifications).

The IC separating columns and precolumns currently available from

Metrohm are listed in section 7.3.2. A test chromatogram and an infor-

mation leaflet is provided with each column. You will find additional in-

formation concerning these columns in the 8.732.2003 Metrohm Mono-

graph «Ion chromatography» and in special "Application Bulletins",

which are available on request free of charge from your local Metrohm

agency.

When you install the column, always ensure that this is inserted cor-

rectly in accordance with the flow direction shown (arrow must point

upwards).

2.8.2 Selection of the sample loop

Selection of the sample loop depends on the separating column used.

Normally, the following sample loops are used:

Anion columns 100 µL

Cation columns 10 µL

Columns for suppressor technique 20 µL

Depending on the instrument version, the following sample loops are

installed in the 733 IC Separation Center:

Version Valve Sample loop Volume

2.733.0010 A 6.2620.120 (steel) 100 µL

2.733.0020 A 6.2620.120 (steel) 100 µL

B 6.2620.100 (steel) 10 µL

2.733.0030 A 6.1825.210 (PEEK) 20 µL

2.733.0120 A 6.1825.220 (PEEK) 100 µL

B 6.1825.230 (PEEK) 10 µL

2.733.0130 A 6.1825.210 (PEEK) 20 µL

If desired, the built-in sample loop can be replaced by one of the sam-

ple loops available as an option (see section 7.3.1).