User's Manual

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8. When the time has elapsed, close the stopcock and remove the syringe and set
aside.
Syringe 5: Rinse Buffer - Degassed
1. Prime the needle. Close the stopcock.
2. Enter the Pump fill port with the refill needle.
3. Open the stopcock and allow the Pump to fill completely with RINSE
BUFFER. When the fluid level stops moving, the Pump is filled. When the
pump is filled, approximately 10 ml of RINSE BUFFER will remain in the
syringe. Leave the RINSE BUFFER in the syringe and leave the syringe in
place for the next step
.
Equilibrate and pull rinse buffer through system
The reservoir pressure in the Pump is equilibrated with outside ambient pressure
and RINSE BUFFER is pulled through the fluid system. This procedure uses the
following volumes in syringes 4 and 5:
• Syringe 4 with RINSE BUFFER. Expel all RINSE BUFFER except 5 ml.
Change refill needle if desired.
• Syringe 5 with residual RINSE BUFFER from previous step.
Syringe 5: Rinse Buffer
1. Verify that the stopcock on syringe 5 is open.
Syringe 4: Rinse Buffer - Residual
1. Prime the needle of syringe 4. Close the stopcock.
2. Obtain a vacuum by pulling back on the plunger until it locks. Press the lock
into the plunger groove to be sure it is firmly secured.
3. Enter the side port with the stopcock closed.
4. Open the stopcock.
5. To open the valve in the Pump mechanism, program and deliver a 4 unit
bolus using the PPC.
6. Observe syringe 4. When at least 1 ml of RINSE BUFFER has entered
syringe 4, close both stopcocks and remove the syringes. If less than 1 ml
enters, repeat the bolus from Step 6. Some Pumps may require 3 successive