User's Manual

121
Remove NaOH and fill with rinse buffer
The NaOH is removed from the Pump and the system is filled with RINSE
BUFFER. This procedure uses the following volumes in syringes 3, 4, and 5:
• Syringe 3 with residual of at least 5 ml NaOH from previous step
• Syringe 4 with 20 ml RINSE BUFFER
• Syringe 5 with 30 ml RINSE BUFFER
Syringe 3: NaOH - Residual
1. Prepare syringe for aspiration.
2. Close the stopcock and obtain a vacuum by pulling back on the plunger until
it locks. Press the lock into the plunger groove to be sure it is firmly
secured.Enter the Pump with the refill needle.
3. Open the stopcock and withdraw all the NaOH. After the NaOH appears to
have stopped rising in the syringe, wait an additional 30 seconds to make
sure all the NaOH and air is removed.
4. When the time has elapsed, close the stopcock, remove the syringe, and
discard.
Syringe 4: Rinse Buffer - Degassed
1. Prime the needle. Close the stopcock.
2. Enter the Pump with the refill needle and syringe.
3. Open the stopcock and allow the Pump to fill completely with RINSE
BUFFER. When the fluid level stops moving, the Pump is filled.
4. Close the stopcock. Remove the syringe.
5. Prepare syringe 4 for aspiration. Obtain a vacuum by pulling back on the
plunger until it locks. Press the lock into the plunger groove to be sure it is
firmly secured.
6. Re-enter the Pump.
7. Open the stopcock and remove the RINSE BUFFER. After the fluid level
stops moving, wait an additional 30 seconds for all the RINSE BUFFER and
air to be removed.