User's Manual

119
The 0.1M NaOH used in this procedure can
cause permanent eye damage. Safety glasses
must be worn during this procedure.
Syringe 2: NaOH - Degassed
1. Prime the needle. Close the stopcock
2. Enter the Pump with the refill needle.
3. Open the stopcock and allow the Pump to fill completely with NaOH. When
the fluid level stops moving, the Pump is filled.
4. Close the stopcock. Remove the syringe.
5. Prepare syringe 2 for aspiration. Remove the air from the syringe. Close
stopcock and obtain a vacuum by pulling back on the plunger until it locks.
Press the lock into the plunger groove to be sure it is firmly secured.
6. Re-enter the Pump.
7. Open the stopcock and remove the NaOH. After the fluid level stops moving,
wait an additional 30 seconds for all the NaOH to be removed.
8. When the time has elapsed, close the stopcock and remove the syringe.
Discard syringe 2.
Syringe 3: NaOH - Degassed
1. Prime the needle. Close the stopcock.
2. Enter the Pump fill port with the refill needle.
3. Open the stopcock and allow the Pump to fill completely with NaOH. When
the Pump is filled, approximately 10 ml will remain in the syringe. Leave this
NaOH in the syringe, and leave this syringe in place for the next step.
!
WARNING