Product Info Part 4

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the bolus from Step 6, some pumps may require 3 successive bolus to
obtain 1 ml.
NOTE: In order to completely dissolve deposits in the
Pump mechanism, it is important to maintain a
vacuum with syringe 1 and deliver the entire
bolus.
8. After the bolus, close the stopcock on syringe 1. Remove both
syringes. Set aside syringe 3 for use in removing the NaOH from the
Pump fill port in the next step. Discard syringe 1.
Remove NaOH and fill with rinse buffer
The NaOH is removed from the Pump and the system is filled with
RINSE BUFFER. This procedure uses the following volumes in syringes
3, 4, and 5:
• Syringe 3 with residual of at least 5 ml NaOH from the previous
step
• Syringe 4 with 20 ml RINSE BUFFER
• Syringe 5 with 30 ml RINSE BUFFER
Syringe 3: NaOH (residual)
1. Prepare syringe for aspiration.
2. Close the stopcock and obtain a vacuum by pulling back on the
plunger until it locks. Press the lock into the plunger groove to be
sure it is firmly secured.
3. Enter the Pump with the refill needle.
4. Open the stopcock and withdraw all the NaOH. After the NaOH
appears to have stopped rising in the syringe, wait an additional 30
seconds to make sure all the NaOH and air is removed.
5. When the time has elapsed, close the stopcock, remove the syringe,
and discard.
Dmp9196021-011_c.book Page 157 Wednesday, April 3, 2002 5:07 PM