User guide

90
GlobalFiler
™
Express PCR Amplification Kit User Guide
Index
volume per reaction 33, 37, 41
GS 500 18
GS 600 18
H
HID Updater 32
Hi-Di formamide, volume per reaction 33
, 37, 41
I
Instrument and software compatibility 16
K
kit
contents 17
description 11
fluorescent dyes 16
instruments for use with 11
loci amplified 12
master mix 17
primers 12
, 17
purpose 11
reagents 17
storage 17
thermal cyclers for use with 76
L
license activation, 6-dye 35, 39
limited product warranty 87
LIZ size standard
about 18
volume per reaction 33
, 37, 41
loci
chromosomal location 12
dye label 12
low-TE buffer 21
lysate, prepare 25
M
master mix
volume per reaction 21
, 24
master mix, volume per reaction 26
materials and equipment 17
multicomponent analysis 16
O
operating systems 16, 31, 35, 39
P
panel
check version 46
import 47
PCR
optimize cycle number 19
perform 28
setup 75
PCR work areas 69
, 75
Prep-n-Go Buffer 11
, 19, 23
primers
volume per reaction 21
, 24, 26
R
reaction mix, for PCR 22, 24, 26
reactions, prepare for PCR 21
, 23, 26
run module, electrophoresis 31
, 35, 39
S
safety
biohazard 79
chemical 78
Safety Data Sheets (SDSs), obtaining 86
size standard 18
spectral calibration, 6-dye 32
, 37, 41
standards for samples 18
stutter
check version 46
import 47
substrates
swab 25
treated paper 20
untreated paper 23
support, obtaining 86
swab
PCR 28
prepare reactions 25
sample preparation guidelines 25
samples 11
types 11