User guide

11
GlobalFiler
™
Express PCR Amplification Kit User Guide
1
Overview
■ Product overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
■ Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
■ Instrument and software overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
■ Materials and equipment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Product overview
Purpose
The GlobalFiler
™
Express PCR Amplification Kit is a 6-dye, short tandem repeat (STR)
multiplex assay optimized to allow direct amplification from the following types of
single-source samples:
• Blood and buccal samples on treated paper substrates without the need for
sample purification.
• Blood and buccal samples collected on untreated paper substrates and treated
with Prep-n-Go
™
Buffer.
• Buccal samples collected on swab substrates and treated with Prep-n-Go
™
Buffer
The GlobalFiler
™
Express Kit amplifies 21 autosomal STR loci (D3S1358, vWA,
D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, D2S441, D19S433, TH01, FGA,
D22S1045, D5S818, D13S317, D7S820, SE33, D10S1248, D1S1656, D12S391, D2S1338,
and the sex-determining marker, Amelogenin, 1 Y STR locus, DYS391, and
1 Y insertion/deletion (Y indel) locus in a single PCR reaction (24 loci total).
Substrate
examples
• Treated paper: Copan NUCLEIC-CARD
™
system or Whatman FTA
®
cards
• Untreated paper: Bode Buccal DNA Collector
™
or 903 paper
• Swab: Copan FLOQSwabs
™
Product
description
The GlobalFiler
™
Express Kit contains all the necessary reagents for the amplification
of human genomic DNA.
The reagents are designed for use with the following instruments and software:
•Veriti
®
96-Well Thermal Cycler (Part no. 4375786)
IMPORTANT! The GlobalFiler
™
Express Kit is validated for use with the Veriti
®
96-well Thermal Cycler (Part no. 4375786) NOT the Veriti
®
96-Well Fast Thermal
Cycler (Part no. 4375305). Please ensure you are using the correct Veriti
®
Thermal
Cycler model.
• GeneAmp
®
PCR System 9700 with the Silver 96-Well Block
• GeneAmp
®
PCR System 9700 with the Gold-plated Silver 96-Well Block