User guide

89
GlobalFiler
™
Express PCR Amplification Kit User Guide
Index
Numerics
3130/3130xl instruments 35
3500/3500xL instruments 31
3730 instrument
allelic ladder base-pair spacing quality assessment
failures 54
set up 31
sizing failures 57
6-dye
license activation 35
, 39
spectral calibration 32
, 37, 41
A
allelic ladder
figure 14
requirements for accurate genotyping 30
volume per reaction 33
, 37, 41
B
bins
check version 46
import 47
biohazard safety 79
blood samples 11
, 19, 20
Bode Buccal DNA Collector 11
, 19, 23
buccal samples 11
, 19, 20, 23
buccal swabs 25
C
chemical safety 78
contents of kit 17
control DNA
about 18
control DNA, about 18
Copan
treated cards 11
D
data collection software 16
DNA
negative control sample preparation 21
, 23, 26
positive control sample preparation 21
, 23, 26, 27
your sample preparation 21
, 23
documentation, related 85
DS-33 Matrix Standard 32
, 37, 41
Dye Set J6 for 6-dye samples 32
, 37, 41
E
electrophoresis
Data Collection Software 31
, 35, 39
prepare samples 33
, 37, 41
references 31
, 35, 39
run module 31
, 35, 39
set up of 3130/3130xl instruments 35
set up of 3500/3500xL instruments 31
set up of 3730 instrument 31
, 39
emission spectra 17
equipment, not included with kit 69
F
Fast model, Veriti thermal cycler, not supported 76
fluorescent dyes 16
FTA cards 11
, 19, 20
G
GeneMapper ID-X Software
analyze project 59
check version of panels, bins, and stutter 46
create analysis method 51
examine and edit project 60
import panels, bins, and stutter 47
overview 43
set up 46
GeneScan size standard
about 18
dye label 16