User guide

75
GlobalFiler
™
Express PCR Amplification Kit User Guide
D
PCR Work Areas
■ Work area setup and lab design . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
■ PCR setup work area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
■ Amplified DNA work area . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Work area setup and lab design
Many resources are available for the appropriate design of a PCR laboratory. If you are
using a GlobalFiler
™
Express Kit for:
• Forensic DNA testing, refer to “Forensic Laboratories: Handbook for Facility
Planning, Design, Construction and Moving,” National Institute of Justice, 1998
• Parentage DNA testing, refer to the “Guidance for Standards for Parentage
Relationship Testing Laboratories,” American Association of Blood Banks, 7th
edition, 2004
The sensitivity of GlobalFiler
™
Express Kit (and other PCR-based tests) enables
amplification of minute quantities of DNA, necessitating precautions to avoid
contamination of samples yet to be amplified (Kwok and Higuchi, 1989).
Also take care while handling and processing samples to prevent contamination by
human DNA. Wear gloves at all times and change them frequently. Close sample tubes
when not in use. Limit aerosol dispersal by handling sample tubes and reagents
carefully.
Note: We do not intend these references for laboratory design to constitute all
precautions and care necessary for using PCR technology.
PCR setup work area
IMPORTANT! These items should never leave the PCR Setup Work Area.
• Calculator
•Gloves, disposable
• Marker pen, permanent
•Microcentrifuge
• Microcentrifuge tubes, 1.5-mL, or 2.0-mL, or other appropriate clean tube (for
Master Mix preparation)
• Microcentrifuge tube rack
• Pipette tips, sterile, disposable hydrophobic filter-plugged
• Pipettors