User guide

67
GlobalFiler
™
Express PCR Amplification Kit User Guide
Appendix A Troubleshooting
A
Data collected on the
3730 instrument with
POP-7
™
polymer: the
D2S441 and D1S1656
markers in some allelic
ladder samples did not
pass the base-pair
spacing quality
assessment
Data was analyzed using the Light
setting for Smoothing
Use the None setting for smoothing after
performing appropriate validation studies.
For more information, refer to the GeneMapper
®
ID-
X Software Version 1.4 User Bulletin
(Pub. no. 4477684 Rev. B), “Known issues: 3730
DNA Analyzer allelic ladder failures”.
Observation Possible causes Recommended actions