User guide

57
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter4 Analyze Data
Set up GeneMapper
®
ID-X Software for data analysis
4
GeneMapper
®
ID-X Software
Note: The GS600_LIZ_(60-460) size standard definition provided with GeneMapper
®
ID-X Software v1.4 has been validated for use with 3130/3130xl, 3500/3500xL, and 3730
instruments. However, when running GlobalFiler
™
Express Kit samples on the 3730
instrument with POP-7
™
polymer, the 60 bp size-standard peak may occasionally be
obscured by the primer peak.
This issue can be addressed by either of the following steps:
• Re-inject samples that fail to recognize the 60 base-pair peak.
• Use the 80 to 460 bp size-standard definition after performing appropriate
validation studies (as a general rule, the 60 base-pair peak is not required for
accurate fragment sizing using the 3rd Order Least Squares sizing method).
For more information, refer to the GeneMapper
®
ID-X Software Version 1.4 User
Bulletin (Pub. no. 4477684 Rev. B), “Known issues: 3730 DNA Analyzer sizing
failures”.
Use the following procedure to create the size standard definition file:
1. Select ToolsGeneMapper
®
ID-X Manager to open the GeneMapper
®
ID-X
Manager.
2. Select the Size Standards tab, then click New.
GeneScan
™
600 LIZ
®
Size Standard v2.0
60, 80, 100, 114, 120, 140, 160, 180, 200, 214, 220, 240, 250, 260, 280, 300, 314, 320, 340, 360,
380, 400, 414, 420, 440, and 460