User guide

55
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter4 Analyze Data
Set up GeneMapper
®
ID-X Software for data analysis
4
GeneMapper
®
ID-X Software
• Size calling method – The GlobalFiler
™
Express Kit has been validated using the
3rd Order Least Squares sizing method. Select alternative sizing methods only
after performing the appropriate internal validation studies.
• Normalization – A Normalization checkbox is available on this tab in
GeneMapper
®
ID-X Software for use in conjunction with data run on the 3500
Series Genetic Analyzers.
Peak Quality tab
settings
IMPORTANT! Perform the appropriate internal validation studies to determine the
heterozygous and homozygous minimum peak height thresholds, maximum peak
height threshold and the minimum peak height ratio threshold for interpretation of
GlobalFiler
™
Express Kit data.
Perform
internal
validation
studies to
determine
settings