User guide

54
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter4 Analyze Data
Set up GeneMapper
®
ID-X Software for data analysis
4
IMPORTANT! Perform the appropriate internal validation studies to determine the
appropriate peak amplitude thresholds for interpretation of GlobalFiler
™
Express Kit
data.
Fields include:
• Peak amplitude thresholds – The software uses these parameters to specify the
minimum peak height, in order to limit the number of detected peaks. Although
GeneMapper
®
ID-X Software displays peaks that fall below the specified
amplitude in electropherograms, the software does not label or determine the
genotype of these peaks.
• Smoothing – 3730 Genetic Analyzer with POP-7
™
polymer only: With the
default Smoothing setting of Light, the D2S441 and D1S1656 markers in some
allelic ladder samples did not pass the base-pair spacing quality assessment. The
instances of spacing failures were significantly reduced by using the None
setting.
For more information, refer to the GeneMapper
®
ID-X Software Version 1.4 User
Bulletin (Pub. no. 4477684 Rev. B), “Known issues: 3730 DNA Analyzer allelic
ladder failures”.
Perform
internal
validation
studies to
determine
settings