User guide

53
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter4 Analyze Data
Set up GeneMapper
®
ID-X Software for data analysis
4
GeneMapper
®
ID-X Software
Allele tab settings
The following settings were used during developmental validation of the GlobalFiler
™
Express Kit:
• In the Bin Set field, select the AmpFLSTR_Bins_v3X bin set.
• GeneMapper
®
ID-X Software v1.0.1 or higher allows you to specify 4 types of
marker repeat motifs: tri, tetra, penta and hexa. You can enter parameter values
for each type of repeat in the appropriate column.
• Specify the appropriate filter settings. To apply the stutter ratios contained in the
AmpFLSTR_Stutter_v3X.txt file, select the “Use marker-specific stutter ratio if
available” check box (selected by default).
Note: Additionally, applying a Global Cut-off Value may reduce the editing
required for single-source sample data.
Perform appropriate internal validation studies to determine the appropriate filter
setting to use.
Peak Detector tab
settings
IMPORTANT! Because of the small amplicon sizes generated by the GlobalFiler
™
Express Kit, the 3rd Order Least Squares Sizing algorithm has been validated for
analysis of GlobalFiler
™
Express Kit data.