User guide

44
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter4 Analyze Data
Overview of GeneMapper
®
ID-X Software
4
• Allelic ladder samples must be labeled as “Allelic Ladder” in the Sample Type
column in a project. Failure to apply this setting for ladder samples results in
failed analysis.
• Injections containing the allelic ladder must be analyzed with the same analysis
method and parameter values that are used for samples to ensure proper allele
calling.
• Alleles that are not in the GlobalFiler
™
Express Kit Allelic Ladders do exist.
Off-ladder (OL) alleles may contain full and/or partial repeat units. An off-ladder
allele is an allele that occurs outside the ±0.5-nt bin window of any known allelic
ladder allele or virtual bin.
Note: If a sample allele peak is called as an off-ladder allele, the sample result
needs to be verified according to the laboratory’s protocol.
New features to
support
GlobalFiler
™
Express Kit data
analysis
GeneMapper
®
ID-X Software v1.4 includes the following new features and updates to
support GlobalFiler
™
Express Kit data analysis. Refer to the GeneMapper
®
ID-X
Software v1.4 New Features and Installation Procedures User Bulletin (Pub. no. 4477684) for
information on other new features of the software.
• Analyzes data generated with the GlobalFiler
™
Express Kit and the J6 dye set.
• The following analysis files are automatically installed and imported into the
software:
– Two new GeneScan
™
600 LIZ
®
size standards:
GS600_LIZ+Normalization_(60-460) and GS600_LIZ_(60-460)
– Panels, bins, table settings, and plot settings updated for the GlobalFiler
™
Express Kit
– CODIS Marker Properties file
• The following analysis files are automatically installed must be imported into the
software before use (see “Import panels, bins, and marker stutter” on page 47):
stutter settings.
• Y-marker analysis. In addition to the ability to analyze autosomal STR and Y-STR
data separately, GeneMapper
®
ID-X Software v1.4 can analyze autosomal and
Y-STR markers in one multiplexed sample. A new marker-level PQV,
Amelogenin Cross Check (ACC) has been added and the Allele Number (AN)