User guide

43
GlobalFiler
™
Express PCR Amplification Kit User Guide
4
Analyze Data
■ Overview of GeneMapper
®
ID-X Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 43
■ Set up GeneMapper
®
ID-X Software for data analysis . . . . . . . . . . . . . . . . . . . . . . 46
■ Analyze and edit sample files with GeneMapper® ID-X Software . . . . . . . . . . . 58
■ Examine and edit a project . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
■ For more information. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Overview of GeneMapper
®
ID-X Software
GeneMapper
®
ID-X Software is an automated genotyping software for forensic
casework, databasing, and paternity data analysis.
GeneMapper
®
ID-X Software v1.4 or higher analyzes 4-dye, 5-dye, and 6-dye data and
is required to correctly analyze data generated using the GlobalFiler
™
Express Kit.
After electrophoresis, the data collection software stores information for each sample
in a .fsa or .hid file. Using GeneMapper
®
ID-X Software v1.4 or higher you can then
analyze and interpret the data from the .fsa or .hid files.
IMPORTANT! Because of the small amplicon sizes generated by the GlobalFiler
™
Express Kit, the 3rd Order Least Squares Sizing algorithm has been validated for
analyzing GlobalFiler
™
Express Kit data. For information, see “Create an analysis
method” on page 51.
Instruments
Refer to “Instrument and software overview” on page 16 for a list of compatible
instruments.
Before you start
When using GeneMapper
®
ID-X Software v1.4 or higher to perform human
identification (HID) analysis with GlobalFiler
™
Express Kits, be aware that:
• HID analysis requires at least one allelic ladder sample per run folder. Your
laboratory can use multiple ladder samples in an analysis, provided individual
laboratories conduct the appropriate validation studies.
For multiple ladder samples, the GeneMapper
®
ID-X Software calculates allelic
bin offsets by using an average of all ladders that use the same panel within a run
folder.
• Allelic ladder samples in an individual run folder are considered to be from a
single run.
When the software imports multiple run folders into a project, only the ladder(s)
within their respective run folders are used for calculating allelic bin offsets and
subsequent genotyping.