User guide

39
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter3 Perform Electrophoresis
Set Up the 3730 instrument for electrophoresis
3
3730 Instruments
Section3.3 3730 instrument
Set Up the 3730 instrument for electrophoresis
Reagents and parts
Appendix B, “Ordering Information” on page 69 lists the required materials not
supplied with the GlobalFiler
™
Express Kit.
IMPORTANT! The fluorescent dyes attached to the primers are light-sensitive. Protect
the primer set, amplified DNA, allelic ladder, and size standard from light when not in
use. Keep freeze-thaw cycles to a minimum.
Electrophoresis
software setup and
reference
documents
The following table lists data collection software and the run modules that you can use
to analyze GlobalFiler
™
Express Kit PCR products. For details on the procedures, refer
to the documents listed in the table.
Obtain and activate
the 6-dye license
for the instrument
1. Confirm that you are running Data Collection Software v4 (HelpAbout).
2. Obtain a 3730 DC v4 6-Dye Module v1 License key. Contact Life Technologies for
information.
3. Ensure that all network cards in the computer are enabled.
IMPORTANT! You can run the 3130 Series Data Collection Software v4 using only
the network cards enabled when you activate the software license. For example, if
you activate the software when your wireless network card is disabled, you will
not be able to run the software when the wireless network card is enabled.
DNA
Analyzer
Operating
System
Data
Collection
Software
Additional
software
Run module References
3730 Windows
®
7
Data
Collection
Software
v4
3130/3730 DC
v4 6-Dye
Module v1
(contact Life
Technologies)
• GeneMapper36_POP7_1
Injection conditions: 2 kv/10 sec
Run conditions: 15 kV/1200 sec
•Dye Set J6
3730/3730xl DNA
Analyzer Getting
Started Guide
(Pub. no. 4478016)