User guide
38
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter3 Perform Electrophoresis
Prepare samples for electrophoresis on the 3130/3130xl instruments
3
Note: For blank wells, add 10 µL of Hi-Di
™
Formamide.
5. Seal the reaction plate with appropriate septa, then briefly vortex and centrifuge
the plate to ensure that the contents of each well are mixed and collected at the
bottom.
6. Heat the reaction plate in a thermal cycler for 3 minutes at 95°C.
7. Immediately place the plate on ice for 3 minutes.
8. Prepare the plate assembly on the autosampler.
9. Start the electrophoresis run.