User guide

35
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter3 Perform Electrophoresis
Set up the 3130/3130xl instruments for electrophoresis
3
3130/3130xl Instruments
Section3.2 3130/3130xl instruments
Set up the 3130/3130xl instruments for electrophoresis
Reagents and parts
Appendix B, “Ordering Information” on page 69 lists the required materials not
supplied with this kit.
IMPORTANT! The fluorescent dyes attached to the primers are light-sensitive. Protect
the primer set, amplified DNA, allelic ladder, and size standard from light when not in
use. Keep freeze-thaw cycles to a minimum.
Electrophoresis
software setup and
reference
documents
The following table lists data collection software and the run modules that can be used
to analyze PCR products generated by this kit. For details on the procedures, refer to
the documents listed in the table.
Obtain and activate
the 6-dye license
for the instrument
1. Confirm that you are running Data Collection Software v4 (HelpAbout).
2. Obtain a 3130 DC v4 6-Dye Module v1 License key. Contact Life Technologies for
information.
3. Ensure that all network cards in the computer are enabled.
IMPORTANT! You can run the 3130 Series Data Collection Software v4 using only
the network cards enabled when you activate the software license. For example, if
you activate the software when your wireless network card is disabled, you will
not be able to run the software when the wireless network card is enabled.
Genetic
Analyzer
Operating
System
Data
Collection
Software
Additional
software
Run modules and conditions References
3130 Windows
®
7
Data
Collection
Software
v4
3130/3730 DC
v4 6-Dye
Module v1
(contact Life
Technologies)
• HIDFragmentAnalysis36_POP4_1
Injection conditions: 3 kV/5 sec
Run conditions: 15 kV/1500 sec
•Dye Set J6
Applied Biosystems
3130 Series Data
Collection Software v4
Getting Started Guide
(Pub. no. 4477796)
3130xl
†
• HIDFragmentAnalysis36_POP4_1
Injection conditions: 3 kV/10 sec
Run conditions: 15 kV/1500 sec
•Dye Set J6
† We conducted validation studies for the GlobalFiler
™
Express Kit using the 3130xl, 3500, or 3500xL configurations.