User guide

32
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter3 Perform Electrophoresis
Set up the 3500/3500xL instruments for electrophoresis
3
Obtain and run the
HID Updater
You can run 6-dye samples on 3500 Data Collection Software v1 or v2. Before running
on either system for the first time, run the HID Updater 3500 DC v2.0
(Part no. 4480670). The HID Updater installs the plate templates, assays, and
instrument protocols needed to run GlobalFiler
™
Express Kit samples. For more
information, refer to the release notes provided with the Updater.
Note: If you have a new instrument installed by a Life Technologies representative, the
updater may have been run during installation.
1. Obtain the HID Updater 3500 DC v2.0 from www.lifetechnologies.com/
supportSoftware, Patches & UpdatesGeneMapper
®
ID-X Software.
2. Exit the 3500/3500xL Data Collection Software.
3. Load the HID Updater Installer CD on the instrument computer.
4. Double click the HID_Updater_3500_DC_SW_2.0.exe file on the CD.
5. When installation is complete, restart the computer.
Perform spectral
calibration
Perform a spectral calibration using the DS-36 Matrix Standard (J6 Dye Set)
(Part no. 4425042). The following figure is an example of a passing 6-dye spectral
calibration.
3500
†
3500xL
†
Windows
®
7
3500 Data
Collection
Software
v2
HID
Updater
3500 DC
v2.0
(Part no.
4480670)
Same as 3500 Data Collection
Software v1 listed above
3500/3500xL Genetic
Analyzer User Guide
(Pub. no. 4476988)
HID Updater 3500
Data Collection
Software v2 Release
Notes
† We conducted validation studies for the GlobalFiler
™
Express Kit using the 3130xl, 3500, or 3500xL configurations.
Genetic
Analyzer
Operating
System
Data
Collection
Software
Additional
software
Plate templates, assays, run
modules, and conditions
(installed with the HID Updater)
References