User guide

31
GlobalFiler
™
Express PCR Amplification Kit User Guide
Section3.1 3500/3500xL instruments
Set up the 3500/3500xL instruments for electrophoresis
3
3500/3500 xL Instruments
Section3.1 3500/3500xL instruments
Set up the 3500/3500xL instruments for electrophoresis
Reagents and parts
Appendix B, “Ordering Information” on page 69 lists the required materials not
supplied with this kit.
IMPORTANT! The fluorescent dyes attached to the primers are light-sensitive. Protect
the primer set, amplified DNA, allelic ladder, and size standard from light when not in
use. Keep freeze-thaw cycles to a minimum.
Electrophoresis
software setup and
reference
documents
The following table lists data collection software and the run modules that you can use
to analyze PCR products generated by this kit. For details on the procedures, refer to
the documents listed in the table.
Genetic
Analyzer
Operating
System
Data
Collection
Software
Additional
software
Plate templates, assays, run
modules, and conditions
(installed with the HID Updater)
References
3500
†
Windows
Vista
®
3500 Data
Collection
Software
v1
HID
Updater
3500 DC
v2.0
(Part no.
4480670)
• Plate templates: 6dye_36_POP4
• Assays: GF+Norm_POP4 and
GF_POP4 which contain instrument
protocol HID36_POP4_J6_NT3200
with the following conditions:
– Run module: HID36_POP4
– Injection conditions: 1.2 kV/
16 sec
– Run conditions: 13 kV/1550 sec
–Dye Set J6
3500/3500xL Genetic
Analyzer User Guide
(Pub. no. 4401661)
HID Updater 3500
Data Collection
Software v2 Release
Notes
3500xL
†
• Plate templates: 6dye_36_POP4_xl
• Assays: GF+Norm_POP4_xl and
GF_POP4_xl which contain
instrument protocol
HID36_POP4xl_J6_NT3200 with
the following conditions:
– Run module: HID36_POP4
– Injection conditions: 1.2 kV/
24 sec
– Run conditions: 13 kV/1550 sec
–Dye Set J6