User guide

30
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter3 Perform Electrophoresis
Allelic ladder requirements
3
Allelic ladder requirements
To accurately genotype samples, you must run an allelic ladder sample along with the
unknown samples.
IMPORTANT! Variation in laboratory temperature can cause changes in fragment
migration speed and sizing variation between both single- and multiple-capillary runs
(with larger size variations seen between samples injected in multiple-capillary runs).
We recommend the above frequency of allelic ladder injections, which should account
for normal variation in run speed. However, during internal validation studies, verify
the required allelic ladder injection frequency to ensure accurate genotyping of all
samples in your laboratory environment.
It is critical to genotype using an allelic ladder run under the same conditions as the
samples, because size values obtained for the same sample can differ between
instrument platforms because of different polymer matrices and electrophoretic
conditions.
Instrument
Number of
allelic ladders
to run
One
injection
equals
Number of samples per allelic
ladder(s)
3500 1 per 3 injections 8 samples 23 samples + 1 allelic ladder
3500xL 1 per injection 24 samples 23 samples + 1 allelic ladder
3130 1 per 4 injections 4 samples 15 samples + 1 allelic ladder
3130xl 1 per injection 16 samples 15 samples + 1 allelic ladder
3730 3 per injection 48 samples 15 samples + 1 allelic ladder