User guide

28
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter2 Perform PCR
Perform PCR
2
These storage recommendations are preliminary pending the results of ongoing
stability studies. The effects of multiple freeze-thaw cycles on the lysate have not been
fully evaluated. Therefore, multiple freeze-thaw cycles are not recommended.
Perform PCR
IMPORTANT! The GlobalFiler
™
Express Kit is validated for use with the Veriti
®
96-well
Thermal Cycler Part no. 4375786 NOT the Veriti
®
96-Well Fast Thermal Cycler (Part no.
4375305). Please ensure you are using the correct Veriti
®
Thermal Cycler model.
1. Program the thermal cycling conditions.
IMPORTANT! When using the GeneAmp PCR System 9700, select the Max
ramping mode. When using the Veriti
®
Thermal Cycler, select the 100% ramping
rate. DO NOT use 9600 emulation mode.
2. Load the plate into the thermal cycler and close the heated cover.
3. Start the run.
4. On completion of the run, store the amplified DNA.
IMPORTANT! Protect the amplified products from light.
Initial
incubation
step
Optimum cycle number
†
† Determine the optimum cycle number for your laboratory according to the instructions on page 19.
Final extension Final hold
Denature
Anneal/
Extend
HOLD CYCLE HOLD HOLD
95°C
1 min
94°C
3 sec
60°C
30 sec
60°C
8 min
4°C
∞
If you are storing the DNA... Then place at...
<2 weeks 2 to 8°C
>2 weeks –15 to –25°C