User guide

18
GlobalFiler
™
Express PCR Amplification Kit User Guide
Chapter1 Overview
Materials and equipment
1
Figure2 Spectral calibration of the six dyes used in the GlobalFiler
™
Express Kit
Materials and equipment
Kit contents and
storage
The GlobalFiler
™
Express Kit contains sufficient quantities of the following reagents to
perform 200 (Part no. 4476609) or 1000 (Part no. 4474665) amplifications at
15 µL/amplification:
IMPORTANT! The fluorescent dyes attached to the primers are light-sensitive. Protect
the primer set, amplified DNA, allelic ladder, and size standard from light when not in
use. Keep freeze/thaw cycles to a minimum.
Table2 Kit Contents and Storage
LIZ
®
TAZ
™
VIC
®
6-FAM
™
SID
™
NED
™
Component Description 200 reaction 1000 reaction Storage
GlobalFiler
™
Express Master Mix
Contains enzyme, salts, dNTPs,
bovine serum albumin, enzyme,
and 0.05% sodium azide in
buffer and salt.
1 tube, 1.2 mL 1 bottle, 6 mL –15 to –25°C upon
receipt, 2 to 8°C after
initial use for up to
1month
GlobalFiler
™
Express Primer Set
Contains forward and reverse
primers to amplify human DNA
targets.
1 tube, 1.2 mL 1 bottle, 6 mL
GlobalFiler
™
Express Allelic
Ladder
Contains amplified alleles.
See Table1 on page 13 for a list
of alleles included in the allelic
ladder.
1 tube, 0.065 mL 1 tube,
0.15 mL
DNA Control 007 Contains 2 ng/µL human male
genomic DNA in 0.05% sodium
azide and buffer
.
†
See Table1 on page 13 for
profile.
1 tube, 0.05 mL 2 tubes,
0.05 mL/tube
† The DNA Control 007 is included at a concentration appropriate to its intended use as an amplification control (i.e., to provide confirmation of
the capability of the kit reagents to generate a profile of expected genotype). The DNA Control 007 is not designed to be used as a DNA
quantitation control and laboratories may expect to see variation from the labelled concentration when quantitating aliquots of the DNA Control
007.