User guide

RVC 2-18 CD& HCL
2 Layout and mode of operation
Version 04/2012, Rev. 1.4 of 25/11/2013 • sb
15
Translation of the original operating manual
Fig. 4: Vapour pressure curves of commonly used organic solvents
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2.2.1.1 Advantages of the rotational vacuum concentration
• No foaming of the samples, minimum loss.
• Several samples can be dried simultaneously.
• Concentration of the sample on the bottom of the vessel. This is
particularly advantageous for small volumes or dilute solutions.
• Suitable for drying aqueous and solvent-containing samples.
• For volumes < 1 ml up to > 3 l.
• Reproducible drying processes thanks to controlled process
parameters, such as the rotor chamber temperature (energy input for
the evaporation) and vacuum (up to the automatic adjustment of the
optimum operating pressure – depending on the pump systems).
• Easy and safe solvent recovery.
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2.2.1.2 Examples of use
• DNA/RNA (mainly water, ethanol, or methanol as solvent)
• Oligo-synthesis, peptides
• PCR (polymer chain reaction)
• HPLC (mainly water/acetonitrile as solvent)
• Isolation/synthesis of organic substances
• Storage and handling of substances (substance libraries)
• Combinational chemistry
• High-throughput-screening (HTS)
• Analysis of food and environmental samples, toxicology
• Forensic applications
• General laboratory evaporation