Technical data

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131.
DNA Analyzer & Sequencer
(APPLIED BIOSYSTEMS/LICOR/EQUIVALENT WORLDWIDE RECOGNIZED
MAKE
SYSTEM)
Fluorescence-based four capillary (upgradable to 16 capillary) Genetic Analyzer / DNA analysis system to support
a variety of molecular applications including sequencing and DNA fragment (AFLP; SSR; SSCP ; SNP) analysis
with resolution of up to 1bp;
Fully automated sample loading to data analysis;
Electrophoresis voltage should range from 100 V up to 15 kV with operating temperature range of the capillaries
suitable for sequencing and DNA fragment analysis;
Argon-ion laser excitation based CCD detection;
System capable of using /96-well plate kits;
Instrument based on liquid flowable polymers as the separation matrix with automatic replenishment of the
capillary;
Multiple lengths capillaries for multiple applications;
Up to 5 dye detection system; upgradeable to incorporate future dye sets and chemistries without any hardware
changes;
Supplied with necessary consumables for 2000rxn DNA fragment analysis and 1000rxn of sequencing (polymer;
buffer; sample tubes; septa; size standards, deionized formaimide;
Accessory spare parts viz. electrodes, valves; syringes etc. should be supplied in duplicate;
The system should be supplied with ddNTP based sequencing kits for long run; DNA Fragment Analysis
Reagents: (Heat Start Taq DNA polymerase, AFLP preselective & selective amplification kits, SNP kits)
Supplied with a computer workstation for instrument control and data analysis loaded with necessary software for
sample ID import, instrument control and data collection; Computer with minimum 80 GB HDD,512 MB RAM,
Built in DVD-RW/CD-RW combo drive and 19inch Flat panel display and high resolution color deskjet printer
should be provided.
Sequencing Software for Basecalling and Gene fragment Analysis software to perform fragment size detection,
linkage & mapping software, heterozygosity detection software; SNP detection software.
Softwares for fragment sizing and alignment of analyzed samples together;
Power Requirements: Voltage 200-220V or 230-240V +/- 10%, 50/60HZ +/- 10 % Current: Maximum 15 amps.
It should be supplied with electric backup system based on lead batteries capable of handling the system in power
failure for atlease 6 hours.
The company supplying the instrument should have proven capability demonstrated in the past in after sales
service and complete support as for as the
Application systems of the machine.
The supplier should have the facility for comprehensive training on the operation of the instrument, software and
Applications Support in India.