Technical data

23
27. Automatic Protein Digestor
cum Maldi Spotter
Digilab Genomic Solutions
The system should have the following features
Reliability which enables a consistent Limit of detection down to 100fmoles of protein.
Environmental Control including a completely enclosed sample processing environment with a built-in HEPA
filtration
The ability to store trypsin in an inactive form for extended periods and the to activate trypsin just prior to use
Peltier chilled reagent storage blocks to prevent degradation of heat labile reagents.
Full plate vacuum flow-through design for Zero carry-over from sample to sample.
The system should have a facility to remove all waste liquids from all reaction blocks without the use of needles or tips
resulting in no possibility that the gel plug sample will be lost to the wash station/waste container.
Reaction block design and temperature control for preparation of 192 samples as simultaneous and/or separate
incubation of each reaction block consisting of 96 samples.
Ability to purge each reaction plate separately.
Ability to simultaneously employ different chemistries for each reaction plate.
Ability to simultaneously employ various digestion-type (in-gel and/or in-solution) methods for each reaction plate.
Reaction Block Temperature Control Ambient to 70 deg C with +/- 1 deg C resolution (3deg C across plate) with
ramping rate of 5deg/sec.
Ability to upgrade up to 8 reaction blocks (each reaction block holds one 96-well reaction plate)
Liquid Handling system consisting of five channel system with capacity of Channels 1-4, (5-500u l), Channel 5 (0.3-
100u l)
Pitch variablity from 9-18 mm. Needles should be open for used alone, with disposable tips or C18 Millipore Micro Zip
Tips.
Tip handling system should comprised of four needles and a tip ejector.
Wash station for simultaneous internal/external needle wash with a speed of 15ml/min
MALDI Spotting to accommodate spotting to MALDI targets from ALL major mass spec manufacturers.
MALDI Spotting Accuracy of +/- 0.5 mm
Should spot 192 samples in less then 2 hours.
Process Tracking should be done with on-board SQL Server database stores calibration information, definitions of
bed layout and protocols.