Technical data

101
184. Flow Cytometer
(BD/Partec/Beckman/ Guava Technologies, USA make)
• Benchtop flow cytometer system capable of performing various molecular & plant biology work (DNA/RNA content
estimation; ploidy; wide cross hybrid detection; Chromosome sorting-karyotyping/painting/mapping; plant pigment analysis;
membrane fluidity/potential/permeability; Intracellular ionized calcium/ Mg; oxidative burst; Cell cycle/viability/division;
protein expression analyses).
• Bench top Flowcytometer with fixed aligned.
488nm Laser (eg. Argon-Ion); Red Diode Laser (635nm) and 360-380 nm UV
laser. Optical system must be capable of performing DNA & chromosome related functions with correlated dyes like Hoechst
etc., Should have capability for at least 6 multiple parameter (i.e. forward scatter; side scatter; At least 4 colors) measurement
of physical and/or chemical characters of a single cell.
• The system should have inbuilt cell sorter and cell concentrator module allowing various sort modes and at high-speed
(>20,000events/second). Cell sorter with high speed will be preferred Fluid sorting Option
• All the necessary software’s and required for complete application of the system should be provided. Predefined programmes
for common molecular biology related applications.
• Computer with minimum 80 GB HDD, 512 MB RAM, Built in DVD-RW/CD-RW, Flat display monitor, and high resolution
color DeskJet printer should be provided., Voltage requirements for all of the above: 220/240 V, 50-60 Hz.
• The company supplying the instrument should have proven capability demonstrated in the past in after sales service and
complete support as for as the application systems of the machine.
• The company should impart specialized and dedicated training for the use of the machine.
• Optional: The system should be upgradable to acquire samples from 96 well plates directly. The system should have option of
automatic cell collection for slide and plate sorting.
OR
Guava Technologies, USA
1. The System should be, Benchtop 96well Flow cytometer with latest Technology and Must be Open system to
accomodate 3 party reagents.
2. The Detection System should have a Solid State Blue Laser for high sensitivity. The Emission wavelength used in the
system should be 488 +/- 10 nm. ,The Fluidics Design of the system should be based on MicroCapillary Flow cell without
Requirement of sheath fluid, to minimize Reagent Consumption/cost. and Micro-Syringe pump. It should be possible
to clean the fluidics on a routine basis by the user, with either De-ionized water and the cleaning fluid supplied by the
Manufacturer or its equivalent., The System should have capacity of Performing upto 6 Parameters Measurement with 4
Fluorescence Detectors and two light scatters. ,Emission : Green (525 nm), Yellow/orange (580-583 nm), Red (675-680
nm) and InfraRed 785 nm, Forward scatter and side scatter., The System should include a PC as an integral Component
of the System. The System should be easy to use, Flexible and inbuild Tray for 96well Microwell plate as well as
Single Tube sampling for walk away Automation., Applicatoin software should have integrated Worklist Editor for
customering autosampling routine as well as easy access to acquisition as well as printing and saving the worklists.
Software should also include Assay Suite for all Turnkey assays available with the system as well as user defined
analysis.
The system should able to work on Low sample size (less than 20ul or 5000 cells ) volume with Minimal Wastage.
185. Fluidized Bed Dryer Lab. Armfield model for granular and powdery materials. Temp. Range : 20 to 200
o
C (± 1
o
C), Capacity : 5 kg,
Power consumption : 3 kw, Timer range : 0 to 10 mi