User guide

6 | Acquiring Images
78 | Gel Doc XR+ and ChemiDoc XRS+ Systems with Image Lab Software
Step 2. Detect Lanes and Bands
To analyze the gel or blot, Image Lab must detect lanes and bands on the image.
Lanes are detected automatically, and then the background is subtracted
automatically. Refer to Using the All Lanes and Single Lane Tools on page 122 for
details. Customize band detection with the following options.
To configure the lane and band detection settings
1. Select the Lane and Band Detection checkbox in the left pane of the Protocol
Setup window.
2. In the right pane, select one of the following lane and band detection options:
 Low Band Detection Sensitivity — this option sets detection at a low
level (25) for images with more prominent bands. Faint bands are not
detected with this setting.
 High Band Detection Sensitivity — this option sets detection at a higher
level (75) for images that are fainter. Extraneous bands can be removed
later, using the Band tools in the Analysis Tool Box. See Lane and Bands
Tools on page 121.