User Manual

P. 770.270.1394 F. 770.270.2389 865 Marathon Parkway Lawrenceville GA 30046

Operation

Once you have assembled all the parts and allowed your microscope to come to room temperature, plug the

power adapter into the microscope and then into the appopriate AC outlet. Note: Excess cold can fog lenses

and cause lamp to fail.

Turn the light on using the black on/off switch on the right side of the base. Next adhust the light intensity using

the brightness control wheel located on the right side just behind the on/off switch.

In order to become acquainted with the controls, choose a specimen slide with which you are familiar. For

example, an old hematology slide or a commercially prepared slide. Place the slide into the slide holder by

pushing back on the thumb guard to open the slide finger. The slide finger closes slowly to eliminate the

possibility of chipping the corner of your slide when it closes.

Move the slide to the center of the stage, by turning the stage control knobs, located just below the stage.

These knobs allow you to move the slide on the X-Y axis (forward/backward and left/right).

The sub-stage iris should then be set to match the aperture of the objective for maximum resolution under each

objective power. You should begin with the 4x or 10x objective.

4x objective 1.0 N.A. (nearly closed) 40x objective - 0.65 N.A. (halfway closed)

10x objective - 0.25 N.A. (1/4 closed) 100x objective - 1.25 N.A. (wide open)

Insert the filter of your choice into the swing-out filter holder beneath the Abbe condenser. Note that many

customers prefer to use the blue filter for routine use.

Once you are comfortably seated, look into the oculars and move the eyepiece tubes together or apart until

you see only one complete circle of light. You have now adjusted your interpupillary distance.

Using the 4x or 10x objectives and the coarse and fine adjustment knobs, bring the specimen into focus. Now,

move the 40x objective into place. You will feel a “clicking” action when the objective is seated properly.

Again, adjust focus for best image. You should also adjust the iris diaphragm (as listed above) for the best

contrast and resolution.

Diopter Adjustment: Since you are using a binocular microscope, you have to adjust the normal difference in

vision between your two eyes. This is a simple but critical adjustment! Close your left eye and look into the right

ocular with your right eye. Adjust the focus to give you the best image. Now look at the ocular tube on the left.

You will see that the left ocular tube has a built-in adjustment ring. Now close your right eye and look with your

left eye into the left ocular. Using the diopter adjustment ring on the left ocular tube, adjust the focus until you

see a clear, focused field.

Friction Adjustment: With repeated use and wear, the stage may drift out of focus. If this happens, you need

only to tighten the tension control ring (located on the right side of the microscope between the coarse

adjustment and the body of the microscope). If the coarse focus is hard to turn, you may choose to loosen the

friction adjustment.

Stage Stop Lever: To help prevent the stage from hitting the objectives, the Revelation III Microscope is

equipped with an adjustable stage stop. Rotate the 100x oil objective into place, and put a slide into the slide

holder. Slowly raise the stage, stopping when the slide makes contact with the objective. Now, turn the stage

stop lever in a clockwise direction toward you. The stage stop lever is located on the left side of the micro-

scope between the coarse adjustment and the body of the microscope.