xPONENT for MAGPIX 4.
© Luminex Corporation, 2011. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any language or computer language, in any form or by any means without prior express, written consent of Luminex Corporation. LUMINEX CORPORATION 12212 Technology Boulevard Austin, Texas 78727-6115 U.S.A. Voice: (512) 219-8020 Fax: (512) 219-5195 xPONENT 4.2 for MAGPIX Software User Manual 89-00002-00-239 rev.
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Table of Contents Chapter 1 Introduction ........................................................................................................1 Safety Precautions .......................................................................................................................................1 Elements of the Software .............................................................................................................................1 Home Page ..............................................
Create a New Batch from a New Protocol ...........................................................................................37 Create a New Multi-Batch ................................................................................................................... 50 Batch Procedures ......................................................................................................................................52 Running a Pending Batch .........................................................
Auto Maintenance (Auto Maint) Tab ........................................................................................................103 System Initialization ...........................................................................................................................105 Running the Performance Verification Routine .................................................................................105 Running Calibration and Verification ......................................................
xPONENT for MAGPIX 4.
Chapter 1: Introduction Safety Precautions DANGER: Samples and waste fluid can contain biohazardous infectious agents. Handle them at Biosafety level 2, as recommended for any potentially infectious human serum or blood specimen in the DCE/NIH manual, Biosafety in Microbiological and Biomedical Laboratories, 1984. CAUTION: Although beads do not contain hazardous or carcinogenic components at toxic levels, they can be toxic if swallowed. In addition, contact with acids liberates toxic gases.
The Home page displays a welcome message, batch creation buttons, Daily Activities shortcuts, and the Installed Protocols list. Return to the Home page at any time by clicking Home in the Navigation toolbar. This page contains the following: • Click to Create a new Batch from a New Protocol - Creates a new batch from a new protocol. This allows you to create a new protocol while you are creating the batch.
• View - Opens the Settings tab of the Protocols page to view the selected protocol. This tab enables viewing the settings, analytes, and plate layout for the selected protocol. • Daily Activities - Contains shortcut buttons to common commands in the xPONENT software: • System Initialization - Opens the System Initialization command in the Auto Maint tab on the Maintenance page. • Shutdown - Opens the System Shutdown command in the Auto Maint tab on the Maintenance page.
Navigation Elements 1. Page - Across the window, above the content pane, are pages. Click a page to go to that part of xPONENT. 2. Tab - On the left side of the window, along the left side of the content pane, are tabs. Click a tab to go to that subsection of the software. 3. Subtab - A tab can have one or more subtabs. These are located below the tab, are smaller, and are identified by the circle on the left end of the subtab. The circle is red when the subtab is open.
9. Delta Cal Temperature 10. XY Status 11. Power Off button System Status Button - This button has two functions: When clicked, it opens the system log. It also displays the current status of the system. If there are no warnings or errors, the System Status button is green with a check mark. If there is a warning, out of calibration condition, or other important user notification, the button is yellow with an exclamation point.
To display quick start information, click the blue question mark at the top of the xPONENT window, then click Quick Start. This displays information about the seven basic steps to start the system.
• Instrument Type • Serial Number • Firmware Version • XYP Heater Temp • Calibration/Verification Status • Delta Calibration Temp • System Temperature • Last CAL Calibration • Last VER Verification • Last Fluidics Test • Drive Fluid • Waste Fluid Items in this list relating to calibration and verification have one of the following states: • Passed - Indicates that the process completed successfully. • Failed - Indicates that the process was not completed successfully. Failed items appear in red.
Adding a New License Key Contact Luminex Technical Support if you have any difficulty saving or adding a new license key. 1. Access the Admin page, then the Licensing tab. 2. Click License (bottom right corner of window). 3. Copy and paste the new key into the License Code field. The License File field remains blank. 4. Click OK. This closes xPONENT, applies the license, and restarts xPONENT.
CAUTION: Use of this software by untrained personnel can result in inaccurate data and test results. Users of xPONENT must read the documentation thoroughly before operating the software. 1. On the System Login tab, type your user ID. 2. If you are using a secure version of the software, type your password. The Home page opens.
1. Adjusting the Sample Probe Height 2. Revive After Storage (Luminex) Routine 3. Calibration/Verification Adjusting the Sample Probe Height Adjust the sample probe height to ensure that the probe drops far enough into the well to acquire sample. NOTE: Ensure that there is no liquid in the wells or reservoirs before adjusting the sample probe height. 1. On the Home page, click Probe and Heater under Daily Activities. The Probe & Heater tab opens. 2.
NOTE: When you adjust and save the probe height settings for all three areas under a plate name, all areas retain the adjustment. WARNING: Correct sample probe height is critical to successful sample acquisition and calibration. Problems with the sample probe can lead to fluid leaks and inhibit sample acquisition. CAUTION: Ensure that the probe height is set correctly before calibrating the system. FIGURE 1.
3. Add 70% isopropanol or ethanol to reservoir RB1 on the off-plate reagent block as indicated on the Cmds & Routines tab. NOTE: The rinse reservoir (RD1) should be empty. 4. Click Retract. 5. Click Run. After the Revive After Storage routine is complete, run the System Initialization routine. System Initialization xPONENT for MAGPIX contains a pre-defined routine to prepare the analyzer for data acquisition. This section describes calibration and performance verification of the system.
Running the System Initialization Routine 1. On the Home page, click System Initialization under Daily Activities. The Auto Maint tab opens. On the Auto Maint tab, the System Initialization option is automatically selected. 2. Verify that the correct lot kit is displayed in the Calibration and Performance Verification drop down field and that the correct reagents (for example, VER and Fluidics reagents) have been added to the off-plate reagent block. 3.
1. On the Home page, click Shutdown. The Auto Maint tab opens, with System Shutdown selected. 2. Click Eject. 3. Fill reservoir RA1 with 3/4 of DI water. 4. Fill reservoir RC1 with 3/4 of 10%-20% household bleach solution. 5. Verify that reservoir RD1 is empty. 6. Click Retract. 7. Click Run. Logging Off and Exiting To log off and exit xPONENT: 1. Click Logoff at the top of the page. 2. When the Confirm dialog box opens, click OK. This opens the Log In page, with Exit on the left tab. 3.
To display system-level help, click the blue question mark at the top of the xPONENT window, then click Contents and Index. The online help opens, where you can navigate to any available topic. To display quick start information, click the blue question mark at the top of the xPONENT window, then click Quick Start. This displays information about the basic steps to start the system.
• Remote Web Monitoring - Enables you to view alerts and system status using a webpage. • LIS - Enables the system to communicate with an external Laboratory Information System (LIS) database. The LIS package enables you to export and import patient result data in ASTM file format. You must have an instrument control license to operate the instrument. For more information about purchasing upgraded packages, or to obtain specific package documentation, contact your vendor.
FIGURE 2. LED Image-Based Analysis 1 Beads in chamber 2 Red LED (635 nm) 3 CCD Imager 4 Green LED (525 nm) Running Assays with MAGPIX General Guidelines WARNING: Modifying or deleting xPONENT system files can cause degradation of system performance. Repair modified or deleted xPONENT system files by uninstalling and re-installing the xPONENT software. Luminex recommends that you contact Luminex Technical Support before uninstalling and reinstalling xPONENT.
CAUTION: This system contains electrical and mechanical components that, if handled improperly, are potentially harmful. Adhere to standard laboratory safety practices. CAUTION: Protection provided by the equipment can be impaired or the warranty voided if the Luminex system is used in a manner not specified by Luminex documentation or Luminex Corporation. Biological Samples CAUTION: Human and animal samples may contain biohazardous infectious agents.
Classification and Reporter Fluorochromes MagPix beads in the calibration kit are used to autofocus the camera and calibrate the CL1, CL2, and RP1 channels. The beads in the verification kit are a mix of 6 different regions that cover the range of the 50-plex map. Both calibration and verification beads are triple-dyed, and the fluorescence signal of these dyes enables classification of each bead set. TABLE 1.
when the analyzer flushes the sample lines after sample acquisition and expels approximately 75 µL of sample back into the well. Examples • If you use a sample volume of 50 µL and aspirate 50 µL, you acquire air bubbles. • If you use a sample volume of 200 µL and a standard sample pickup of 50 µL, the well overflows when the analyzer washes the sample lines after acquisition and expels fluid back into the well, because the amount of fluid expelled back into the well is approximately 75 µL.
See the recommended consumables list on the Luminex website at http:// www.luminexcorp.com/Support/index.htm and click Recommended Materials from the Support Resources section for more information.
xPONENT for MAGPIX 4.
Chapter 2: Samples Page Samples Page Functionality Samples > Samples Use this tab for the following: • Click Create New Samples to display the Create Samples subtab, on which you can create a new sample. • View sample lists, including a list of protocols with version number and the number of samples associated with each protocol.
• Click Details to display the Edit Samples subtab, on which you can view or edit sample details for the selected protocol. • Click Create Batch to name the LIS batch for a protocol. This opens the Batches page, Batches tab, with the following subtabs displayed: • Protocol • Stds & Ctrls • Plate Layout Edit Samples and Create Sample Subtab Samples > Samples > Edit Samples or Create Sample Click Create Samples on the Samples tab to display this subtab. Use this subtab to type and view sample information.
The following Delete, New, Edit, and Undo buttons only display depending on actions taken in the Create Sample tab. Delete - Deletes a highlighted sample. New - Creates a new sample. Edit - Edits a highlighted sample. Undo - Reopens the Create Sample tab without saving any changes made using the Edit or New buttons. Save - Saves changes made to the Sample list. Close - Returns to the Samples tab. Creating a New Sample List Follow these steps to create a new sample list.
1. Open the Samples page. 2. In the Sample Lists section, select the protocol you are using for the sample list, then click Create New Samples. The Create Sample tab opens. 3. In the ID box, type the sample ID. 4. Type a patient first name in the First name box (optional). 5. Type a patient last name in the Last name box (optional). 6. To add a comment about the sample, type it in the Comment box; this is optional. 7. Click Save to add the sample to the Sample list. xPONENT for MAGPIX 4.
8. To add more samples, click New. Repeat Steps 3-7 until you have added all of the samples you want in your samples list. 9. Once you have added all the desired samples, click Close. NOTE: Samples can also be added using an LIS. Editing a Sample List 1. Open the Samples page. 2. In the Samples list section, choose the protocol you want to edit, then click Details. The Edit Samples subtab opens.
3. Click a sample, then use the Move arrows to move it up or down in the sample list, changing the order in which they will be acquired. 4. To add a new sample to the list, click New, then perform the following steps: a. In the ID box, type the sample ID. b. Type a patient first name in the First name box (optional). c. Type a patient last name in the Last name box (optional). d. To add a comment about the sample, type it in the Comment box; this is optional. e.
Chapter 3: Batches Page Batches Page Functionality Batches > Batches Options on the Batches tab on the Batches page are: • Create a New Batch from an existing Protocol • Create a New Batch from a new Protocol • Create a New Multi-Batch Depending on your selection, this page displays the following tabs: • Protocols - Displays when Create a New Batch from an existing Protocol is clicked.
• Analytes - Displays when Create a New Batch from a new Protocol is clicked. • Plate Layout - Displays when Create a New Batch from an existing Protocol and Create a New Batch from a new Protocol are clicked. • New MultiBatch - Displays when Create a New Multi-Batch is clicked. NOTE: These tabs (except New MultiBatch) are sequential. You must complete each screen in a specific order.
1. Open the Batches page. 2. Click one of the following: • Create a New Batch from an Existing Protocol • Create a New Batch from a new Protocol - if you select this option, you can save the prootocol and/or stds/ctrls information. • Create a New Multi-Batch 3. Type the batch name in the Batch Name box. 4. Type an optional description of the batch in the Enter Optional Description box. 5. If you are creating a batch from an existing protocol, select the protocol in the list. Click Next.
Use this tab to name a batch, type a batch description, select a protocol, and view active reagents. This tab contains the following: • Batch Name/Description - Used to name and describe a batch. • Select a Protocol - Contains the protocol name, version, manufacturer, and creation date for each protocol. • Active Reagents - Displays assay and control lots/kits associated with the selected protocol.
Use this tab to apply a kit or lot to the batch.
• Apply Std/Ctrl Kit - Opens the Select Std/Ctrl Kit dialog box. The dialog box displays the Std/Ctrl Kit Lot #, Std/Ctrl Kit Name, Expiration, and Manufacturer for the kit. Select a Std/Ctrl kit from the list and then click OK to close the dialog box. The kit information will display in the boxes to the right of the Apply Std/Ctrl Kit button. The selected kit must be associated with the same analyte names.
• Assay Control Information - Lists the selected control reagents. The list displays the Reagent, Name, Lot Number, Expiration, and Manufacturer. Existing control lot information can be applied or new information can be typed manually. • Apply Ctrl Lot - Opens the Select Lot dialog box. Select a lot from the list and then click OK. • Show Concentration - Expected, Low, and High set the expected, lowest, or highest acceptable concentration of the analyte in the sample.
• Import List - Opens the Open dialog box to import an existing command sequence list. • Replicate Count - Defines a quantity of replicate sets from one to nine. NOTE: Replicate count selection must be made before adding a well command. • Grouping - Selects the sequence in which the replicates are laid out in plate wells. NOTE: Grouping selection must be made before adding a well command. The options are: • 123123123. . . Lays out one of each replicate set at a time in numerical order. • 111222333. . .
Clicking either Pre Batch Routine or Post Batch Routine opens the Commands and Routines dialog box, where you can select the command or routine you want before or after running the batch. Clicking Add after selecting a well opens the same box for you to select a command or routine for that well. Clicking Delete after selecting a well deletes any commands or routines associated with that well. • Plate - Specifies the plate to display in the plate image in the list.
5. Define the settings in the Acquisition Settings section. These are Volume, XY Heater (enable/disable and set temperature), Plate Name, and enable/disable Sample Wash. NOTE: Final washes are required for proper analysis. If you are not performing a final wash step on your plate before acquisition on the MAGPIX instrument, enable Sample Wash here. This automatically washes each sample.
16. Click Single Step to acquire the first well, then pause acquisition. 17. Click Run Batch to start acquisition, or Save to save the batch for a later time. You can also save the protocol and/or standard and control information by clicking Save Prtcl. NOTE: If the batch spans more than one plate, the tray ejects automatically when all defined wells have been acquired. A dialog box displays prompting you to insert the next plate.
• Name box and box for description - Type a name and a description in the appropriate boxes. • Acquisition Settings - Use this section to set the following: • Volume - This is the volume the instrument will aspirate into the system for analysis. Type the desired sample volume in microliters. Use values from 20 to 200 µl. To avoid air intake, add at least 25 µl to the sample well in addition to the sample size. The default value is 50 µl. • XY heater - Select Enabled to enable the XY heater.
• Analysis Settings - Displays the analysis type to be used for the batch. Use this section to set the analysis type, set the number of standards and controls, select an external analysis program, and choose whether or not to analyze results while acquiring samples. Analysis Type - Use this list to choose from the following analysis types: • None - No analysis. Select if you have your own data post-processing program and want to obtain only fluorescent intensity results.
• Use External Analysis Program - Select this check box to use a third-party program to analyze the data. The Analysis Program list becomes active when this is selected. • Analysis Program - Use this list to select which program to use for data analysis. • Cancel - Click to return to the main Batches tab. • Next - Click to advance to the Analytes tab. Analytes Subtab Batches > Batches > Analytes Use this tab to select or edit analytes used in the batch or protocol.
• Analytes grid - A grid representing each analyte from 12 to 78. Select All selects all analytes, and Deselect All deselects all analytes. Click a numbered analyte to select it; click the analyte again to deselect it. You can also click and drag to select groups of analytes. Selected analytes are red. Deselected analytes are gray. An analyte marked as an intra-well normalization bead is blue. • Default Analysis - The default analysis changes based on the Analysis Type selected in the Settings tab.
• Count - Type the desired bead count for the analytes by clicking in the Count box. If each selected bead set does not acquire this number of events, a warning is added to the log that not enough bead events were acquired. If you select bead sets that are not present, the analyzer continues to acquire, trying to reach the number of events per bead for bead sets that are not in the sample. Therefore, choose only the bead sets present in your sample.
• Selected Analytes list - Selected analytes are displayed in a list on the right side of the analyte grid. This list includes the following information: • Name - The name of the analyte. Click and type to rename the analyte. • Analysis - To change the type of analysis for an analyte, click this field to open the Analysis Settings dialog box and select another analysis from the list. In the Analysis Settings dialog box: FIGURE 4. Analysis Settings Dialog Box 1. Select a method from the Method list. 2.
• Cancel - Click Cancel to return to the Batches tab. • Back - Click Back to return to the Settings tab. • Next - Click to go to the next tab. If the Analysis Type selected in the Settings tab was None or Allele, this takes you to the Plate Layout tab. If the Analysis Type selected was Quantitative or Qualitative, this button takes you to the Stds & Ctrls tab. Standards and Controls (Stds & Ctrls) Subtab Batches > Batches > Stds & Ctrls Use this tab to apply a kit or lot to the batch.
• Apply Std/Ctrl Kit - Opens the Select Std/Ctrl Kit dialog box. The dialog box displays the Std/Ctrl Kit Lot #, Std/Ctrl Kit Name, Expiration, and Manufacturer for the kit. Select a Std/Ctrl kit from the list and then click OK to close the dialog box. The kit information will display in the boxes to the right of the Apply Std/Ctrl Kit button. The selected kit must be associated with the same analyte names.
• Assay Control Information - Lists the selected control reagents. The list displays the Reagent, Name, Lot Number, Expiration, and Manufacturer. Existing control lot information can be applied or new information can be typed manually. • Apply Ctrl Lot - Opens the Select Lot dialog box. Select a lot from the list and then click OK. • Show Concentration - Expected, Low, and High set the expected, lowest, or highest acceptable concentration of the analyte in the sample.
• Import List - Opens the Open dialog box to import an existing command sequence list. • Replicate Count - Defines a quantity of replicate sets from one to nine. NOTE: Replicate count selection must be made before adding a well command. • Grouping - Selects the sequence in which the replicates are laid out in plate wells. NOTE: Grouping selection must be made before adding a well command. The options are: • 123123123. . . Lays out one of each replicate set at a time in numerical order. • 111222333. . .
Clicking either Pre Batch Routine or Post Batch Routine opens the Commands and Routines dialog box, where you can select the command or routine you want before or after running the batch. Clicking Add after selecting a well opens the same box for you to select a command or routine for that well. Clicking Delete after selecting a well deletes any commands or routines associated with that well. • Plate - Specifies the plate to display in the plate image in the list.
your plate orientation) as long as space is available on the plate. You can also select a well first, which places the next batch in your chosen location. If space limitations create an overlap, an error message appears. Results for each batch are saved as individual batch files. NOTE: You cannot add a batch that forces multiple plates to a multi-batch operation. When creating or adding batches, ensure your batches fit on one plate. All batches must use the same plate name previously defined and adjusted.
• New Batch - Opens the Create New Batch tab. Create your new batch. Click Save to return to the New Multibatch tab. • Add - Opens the Select Pending Batch box. Add a batch from the available options, including batches newly created. The selected batch then appears on the plate layout. If the batches selected do not fit on the plate, a Multi-Batch error dialog box opens, indicating you must edit one or more of the selected batches.
1. Open the Batches page. 2. Click Import. The Import Batch dialog box opens. Batch files are MDF files. 3. Click Browse to open the Select File dialog box. Navigate to the batch file you want to import, then click Open. 4. Click OK in the Import Batch dialog box. The batch displays in the Pending Batches list. Exporting a Batch 1. Open the Batches page. 2. In the Pending Batches section, click the batch you want to export, then click Export. The Export Batch dialog box opens.
3. Edit the information as needed on the Protocol, Std & Ctrls, and Plate Layout tabs. For the tab, confirm that the plate layout conforms to your specific assay instructions. 4. Click Save on the Plate Layout tab. NOTE: Batches saved to a multi-batch cannot be edited or deleted unless they are removed from the multi-batch. However, you can edit the multi-batch itself. To remove a batch from a multi-batch, click on a well in the plate layout, and click Remove.
Chapter 4: Results Page Results Page Functionality Results > Current Batch Once data is collected in a batch, observation and analysis take place in the Results page.
• Current Batch - View statistics for the current run and progress per well. • Saved Batches - View information about already processed batches and, if necessary, replay them or recalculate their data.
You can direct the system to acquire samples in replicate regardless of batch type. For qualitative batches, qualitative results for replicates are averaged and the reported interpretation is determined from this replicate average. Replicates in quantitative batches are based on a standard curve that is generated by either the “Fit of all standards” or “Mean of replicates”. The default is “Fit of all standards”. Unknown samples are calculated from the standard curve.
plot data. The statistics available on this tab are intrawell bead statistics. They do not describe replicate well assay results. There are four maximize buttons in this window, one for each major pane. Click the appropriate one to maximize the pane. After clicking, the clicked button becomes a minimize button. Click minimize to return the pane to its standard size. NOTE: The buttons on this tab change based on settings chosen on other application pages.
• Statistic - To view a particular statistic for analytes in a batch, select one of the options on the drop-down list. The statistical options displayed change according to the type of analysis. NOTE: Trimmed statistics (indicated by *) remove the lower and upper five percent of the extreme statistic values, then use the remaining values for the Mean, Standard Deviation, or %CV calculations.
• Control Range - High - The highest value for an assay control used to determine pass/ fail criteria for an assay. • Normalized Net Median - For each analyte in a well the Normalized Net Median (NNM) = (net median of analyte) / (net median of normalization bead) • Units - The unit of measure for an analyte, for example, pg/mL. • Analyte - Contains a list of analytes run in the batch. Select an analyte to view all statistics for that analyte.
• Progress - Click to display real-time progress of the well acquisition. Analyte counts are displayed in a dynamic bar graph as they are acquired. The scroll bar at the bottom of the Progress display scrolls through the analyte list. A zoom feature on the left of the display enables you to enlarge the image. • Default - Appears only when the progress display is active. Click to return to the dot plot display.
• Filter - Click Filter to open the Filter Setup dialog box. This dialog box lets you choose the saved batches you want to display in the Completed Batches list, based on options you select or clear in these check boxes: • Batch Name • Protocol • Batch Status • Lot ID • Kit ID • Analyte • Sample ID • First Name • Last Name • User ID • Date • Reset - Clears all check boxes. • OK - Closes the dialog box and applies any changes you made. • Cancel - Closes the dialog box and cancels any changes that you made.
• Save Prtcl - Opens the Save Protocol dialog box, displaying the kit information for the selected batch. • Plate Layout - Opens the Report dialog box, which contains the Batch Plate Layout Report. • Approve - Opens the Batch Approval Confirmation dialog box to approve the selected batch. Only approved batches can be transmitted to the LIS. If your software is licensed for LIS use, you can transmit batches to the LIS from the Sample Results tab.
• Replay - Opens the Select Replay Mode dialog box. This box enables you to use the data stored in the run files from the initial acquisition to reprocess a batch, creating a new batch output file. A batch can be reprocessed multiple times. When you replay or recalculate a batch, you perform the same steps to create the batch as you did when you created the batch the first time. This sequence varies based on whether or not you created a new batch from a new protocol or a new batch from an existing protocol.
Click an analyte to open the Analysis Settings dialog box from which you can select a new analysis setting for the analyte. • Progress - Click to display real-time progress of the well acquisition. Analyte counts are displayed in a dynamic bar graph as they are acquired. The scroll bar at the bottom of the Progress display scrolls through the analyte list. A zoom feature on the left of the display enables you to enlarge the image.
Replaying or recalculating a large batch can take 1 hour or more to complete. Batch replay cannot be stopped while in progress. Allow adequate time for the operation to complete. The operation is complete when all progress bars have disappeared. A batch can be reprocessed multiple times. If the system crashes but the plate finished, the data can be recovered by replaying the batch. The initial batch data and output file always remain intact and unchanged.
This subtab displays the following features: There are three maximize buttons in this window, one for each major pane. Click the appropriate one to maximize the pane. After clicking, the clicked button becomes a minimize button. Click minimize to return the pane to its standard size.
• Statistic - To view a particular statistic for analytes in a batch, select one of the options on the drop-down list. The statistical options displayed change according to the type of analysis. NOTE: Trimmed statistics (indicated by *) remove the lower and upper five percent of the extreme statistic values, then use the remaining values for the Mean, Standard Deviation, or %CV calculations.
• Control Range - High - The highest value for an assay control used to determine pass/ fail criteria for an assay. • Normalized Net Median - For each analyte in a well the Normalized Net Median (NNM) = (net median of analyte) / (net median of normalization bead) • Units - The unit of measure for an analyte, for example, pg/mL. • Analyte - Contains a list of analytes run in the batch. Select an analyte to view all statistics for that analyte.
• Invalidate - Invalidates an entire selected row or cell in the Results table. The selection will turn red when invalidated. Select the same item and click Validate to remove the invalidation status. • Close - Closes the batch and reopens the Saved Batches tab. Validate Standards Your xPONENT® system administrator must give you privileges to validate standards if you are using the Secure xPONENT package. All standards are assumed to be valid unless explicitly invalidated. 1. Open the Results page. 2.
When you click the Settings subtab on the Saved Batches page, a report opens. This report displays: • A date and time stamp at the top of the report • < and > scroll buttons so that you can view pages in the report • Calibration State • Machine Information • Assay Lots Used Viewing Batch Settings 1. Open the Results page, then open the Saved Batches tab. 2. Click Saved Batches, then click the batch for which you want to view details. 3. Click Open, then click the Settings tab. 4.
This tab displays a log of the activity which occurred during the acquisition of the selected batch; you can print the log. The following information is displayed about each activity: • Date • Message • Code Log entries display yellow if a well was acquired but there was a possible problem, and red if acquisition failed. • Print - Prints the log. • Export - Opens the Save As dialog box to save the batch log file. Select a location and click Save. • Close - Reopens the Saved Batches tab.
• < and > Arrows - Scroll left to right through the sample details. • ^ and v Arrows - Scroll up and down through the sample details. • Transmit - For systems configured for LIS transmission, select a single analyte or the entire sample and click Transmit to send the results. • Close - Reopens the Saved Batches tab. In addition, the following information is shown on this tab: • SampleID • Samples Status • Analyte Viewing Sample Details 1. Open the Results page, then open the Saved Batches tab. 2.
• Filter - Opens the Filter Setup dialog box. Batch-Specific Details • Batch Name • Protocol • Batch Status • Lot ID • Kit ID • Analyte Sample Details • SampleID • First Name • Last Name Others • User ID • Date • Reset • OK / Cancel • Clear - Click to turn off the filter. • Completed Samples - Displays Name, Protocol, Sample Count, Date, Status, and User information for each batch displayed in this list. • Transmit - Transmits a batch to a LIS if xPONENT is connected to one.
Reports Tab Results > Reports Use this tab to view, generate, and print reports. • Report and Type lists - Report lists the categories of reports. The selections in the Type list change depending on the selection you made from the Report list. Depending on the choice you make, various other changes occur on the Reports tab: • Batch Reports - A list of batches opens, so that you can select one. In addition, a Select Analytes box opens to the right of the reports list.
• Select Analyte arrows - This feature is directly below the Report list. Use the left and right arrows to display information for individual analytes of those selected for the report. • Page arrows - Use the arrows to scroll through the pages being displayed. • Save All - Click to open the Browse For Folder dialog box. Select a location to save the file, and click OK. This file includes all selected analytes. • Print All - Click to print the analyte information for all the analytes in the report.
Chapter 5: Protocols Page Protocols Page Functionality Protocols > Protocols The Protocols page enables you to create or import a new protocol, or select an existing protocol from the Installed Protocols list. The following installed protocol information is shown on this page: • Name • Version • Manufacturer • Date Click the Create New Protocol button or click Stds/Ctrls to move to those pages.
There are action buttons at the bottom of the page. Most of these buttons are not displayed unless you are working with a saved protocol. While you are creating a protocol, only Cancel and Next buttons are displayed.
13. To set a bead count and the units for a single analyte, click in the Units and Count columns directly to the right of the analyte grid, and type a units value and bead count. 14. Click in the Call % column and type a value to set an individual analyte's call percentage. 15. Click Next. The Plate Layout tab opens. • To add well commands, select the appropriate wells and mark them as unknown, standard, control, background, or wash.
13. To set a bead count and the units for a single analyte, click in the Units and Count columns directly to the right of the analyte grid, and type a bead count and units value. 14. To change the default analysis for all analytes, click Change. The Analysis Settings dialog box opens. 15. In the Analysis Settings dialog box, select the analysis method from the Method list, and the weighting in the Weight Type list. Click Apply to All Analytes to apply the selection to all analytes. 16.
27. Click Next. The Plate Layout tab opens. • To add well commands, highlight the appropriate wells and mark them as unknown, standard, control, background, or wash. You can also delete commands that you have added and change the starting location on the plate. If you want to run in replicate, change the Replicate Count to the appropriate number and the Grouping to your preferred grouping method. NOTE: Select the Replicate Count and Grouping settings before adding a well command.
default Post Batch routine can be changed in the Batch Options tab of the Admin page. Creating a Qualitative Assay Protocol You define each Qualitative assay protocol using a single kit. The kit must contain one standard. The standard is assigned a “Quali” value for each test. Controls are optional, but are recommended for judging the acceptability of batch results. To create a Qualitative Assay Protocol: 1. Open the Protocols page, then open the Protocols tab. Click Create New Protocol.
13. Click Next. The Plate Layout tab opens. • To add well commands, highlight the appropriate wells and mark them as unknown, standard, control, background, or wash. You can also delete commands that you’ve added, and change the starting location on the plate. If you want to run in replicate, change the Replicate Count to the appropriate number and the grouping to your preferred grouping method. • To add maintenance commands, choose a command from the list.
6. Define the plate layout. 7. Click Save. Exporting a Protocol 1. Open the Protocols page, then open the Protocols tab. 2. Select a protocol. 3. Click Export. The Save as dialog box opens. 4. Select a location to export the file to, and click Save. Importing a Protocol 1. Open the Protocols page, then open the Protocols tab. Click Import. 2. In the Open dialog box, navigate to the protocol file you want to import, then click Open. 3. The imported protocol displays in the Installed Protocols list.
3. If the protocol uses standards, type the appropriate information for each standard in the Assay Standard Information section. In each analyte column, type the expected concentration for the analyte. 4. Alternatively, click Apply Std/Ctrl Kit and select a lot from the Select Lot dialog box. Click OK to apply the lot. 5. If your batch uses controls, select Expected, Low, or High from the Show Value options. Use the Apply Values arrows to apply values down or across the range of analytes. 6. Click Save.
3. Type the name of the kit in the Name box, the lot number in the Std/Ctrl Kit Lot# box, the expiration date using MM/DD/YY format in the Expiration box, and the manufacturer in the Manufacturer box. 4. Click Apply Std Lot if you want to apply a standard lot. The Select Lot dialog box opens. Click a lot and select OK. 5. Click Apply Ctrl Lot to apply a control lot. The Select Lot dialog box opens. Select a lot and click OK. 6.
• Import • Export • Exit • View Settings Subtab Protocols > Protocols > Settings Click Create a New Protocol on the Protocols tab to open the Settings subtab. Name and description boxes - Type the name and description in the appropriate box. Acquisition Settings • Volume - This is the volume the instrument aspirates into the system for analysis. Type the desired sample volume in microliters. Use values from 20 to 200 µl.
• Analysis Type - Use this list to choose from the following analysis types: • None - No analysis. Select if you have your own data post-processing program. You cannot apply standards or controls when you select None. You cannot analyze acquisitions with this setting. • Qualitative - Qualitative analysis determines results as either positive or negative, reactive or non-reactive. The software is flexible in defining custom results ranges, such as negative, low positive, or high positive.
• Fit of all Standards - The standard curve will be determined by using each individual standard replicate when calculating the standard curve. For example, if you run duplicates of a seven-point standard curve, the software will calculate the standard curve by using 14 points. Applies only to quantitative analysis. • Mean of Replicates - The standard curve will be determined by averaging the individual standard replicates when calculating the standard curve.
6. If you selected Lum Qual in the Formulas list, edit the values for each range under Negative, Low Positive, or High Positive. If you selected Adv Qual in the Formulas list, edit Negative, Low Positive, Moderate Positive, or Strong Positive. Select the check box in the Inclusive column to include the value in the range. If you clear the check box, the value will be one unit higher than the low value, and one unit lower than the high value. 7. Click Add Range if you want to add a new range.
• Plate Image - This is a representation of the plate. Each well appears as a circle on the grid. Well commands appear in the appropriate circles as you assign them to wells on the plate. The Plate Navigation section in the lower right part of the window can be used to display all the wells in the plate image. Click and drag in the Plate Navigation box to display different portions of the plate. • Command Sequence - Contains the command sequence for the active plate.
• Add - Opens the Commands and Routines dialog box so you can select a command or routine. • Delete - Deletes the selected command or routine. • Post Batch Routine - Opens the Commands and Routines dialog box so you can select a post-batch command or routine. • Pre Batch Routine - Opens the Commands and Routines dialog box so you can select a pre-batch command or routine. • Plate - Specifies the plate you want to display in the plate image in the list.
• Plate Navigation - Displays a smaller plate image for the current batch. Click and drag in the box to select wells to display. • Off Plate Area - Designates an alternate location for maintenance commands in the Commands and Routines list. • Cancel - Returns to the main Protocols tab without saving. • Back - Returns to the Analytes tab • Save - Saves the protocol and returns to the main Protocols tab.
• Apply Std/Ctrl Kit - Opens the Select Std/Ctrl Kit dialog box. The dialog box displays the Std/Ctrl Kit Lot #, Std/Ctrl Kit Name, Expiration, and Manufacturer for the kit. Select a Std/Ctrl kit from the list and then click OK to close the dialog box. The kit information will display in the boxes to the right of the Apply Std/Ctrl Kit button. The selected kit must be associated with the same analyte names.
Use this tab to select or edit analytes used in the batch or protocol.
• Analytes grid - A grid representing each analyte from 12 to 78. Select All selects all analytes, and Deselect All deselects all analytes. Click a numbered analyte to select it; click the analyte again to deselect it. You can also click and drag to select groups of analytes. Selected analytes are red. Deselected analytes are gray. An analyte marked as an intra-well normalization bead is blue. • Default Analysis - The default analysis changes based on the Analysis Type selected in the Settings tab.
• Count - Type the desired bead count for the analytes by clicking in the Count box. If each selected bead set does not acquire this number of events, a warning is added to the log that not enough bead events were acquired. If you select bead sets that are not present, the analyzer continues to acquire, trying to reach the number of events per bead for bead sets that are not in the sample. Therefore, choose only the bead sets present in your sample.
• Selected Analytes list - Selected analytes are displayed in a list on the right side of the analyte grid. This list includes the following information: • Name - The name of the analyte. Click and type to rename the analyte. • Analysis - To change the type of analysis for an analyte, click this field to open the Analysis Settings dialog box and select another analysis from the list. In the Analysis Settings dialog box: FIGURE 6. Analysis Settings Dialog Box 1. Select a method from the Method list. 2.
• Cancel - Click Cancel to return to the Batches tab. • Back - Click Back to return to the Settings tab. • Next - Click to go to the next tab. If the Analysis Type selected in the Settings tab was None or Allele, this takes you to the Plate Layout tab. If the Analysis Type selected was Quantitative or Qualitative, this button takes you to the Stds & Ctrls tab. Standards and Controls (Stds & Ctrls) Tab Protocols > Stds & Ctrls Use this tab to delete, edit, export, import, and create standards and controls.
Select All Lots in the Show selections to display the following information about each installed lot: • Reagent • Lot # • Expiration • Name • Manufacturer • Protocol • Versions • Std/Ctrl Kit # • Std/Ctrl Kit Name Select Std/Ctrl Kits Only in the Show selections to display the following information about the Installed Std/Ctrl Kits: • Std/Ctrl Kit # • Std/Ctrl Kit Name • Expiration • Manufacturer • Created with Protocol • Version • Delete - Deletes a selected lot.
Click Create New Std/Ctrl Lots on the Standards and Controls (Stds & Ctrls) tab of the Protocols page to open the Standards and Controls Details tab. Use this tab to create or edit a kit.
• Apply Std/Ctrl Kit - Opens the Select Std/Ctrl Kit dialog box. The dialog box displays the Std/Ctrl Kit Lot #, Std/Ctrl Kit Name, Expiration, and Manufacturer for the kit. Select a Std/Ctrl kit from the list and then click OK to close the dialog box. The kit information will display in the boxes to the right of the Apply Std/Ctrl Kit button. The selected kit must be associated with the same analyte names.
Chapter 6: Maintenance Page Use the Maintenance page to maintain and calibrate your system.
This tab contains routines to initialize, verify, and maintain the analyzer. Automated Maintenance Options - Provides buttons for commonly used maintenance routines for the machine. Select from the following: • System Initialization - Select this routine to perform System Initialization. • Performance Verification - Select this routine to use with the Performance Verification kit. • Calibration/Verification - Select this routine to use with the Calibration/Verification kit.
System Initialization xPONENT for MAGPIX contains a pre-defined routine to prepare the analyzer for data acquisition. This section describes calibration and performance verification of the system. Calibrator magnetic beads are used to normalize the settings for the reporter channel and classification channels. Verification magnetic beads are used to verify calibration and optical integrity of the system. Fluidics beads are used to assess well-to-well carryover.
Running Calibration and Verification Before running Calibration/Verification from the Auto Maint tab, you need to import Cal and Ver kit information. Perform that procedure from the Lot Management tab. SeeImporting CAL or VER Kits. For information about what calibration and verification accomplish, see Magpix Technology. For complete procedures for performing calibration and verification, refer to the package inserts that are included with Luminex Calibration and Verification kits.
• Active Reagents - Use this section to change calibration or performance verification kits, lot numbers and expiration dates, or to delete a kit. • Calibration Kit - Select a calibration kit from this list. Details of the selected kit display in the Lot Type, Active Lot Number, and Expiration Date fields. • Performance Verification Kit - Select a performance verification kit from this list. Details of the selected kit display in the Lot Type, Active Lot Number, and Expiration Date fields.
Deleting CAL and VER Kit Information 1. Open the Maintenance page, then open the Lot Management tab. 2. In the Active Reagents section, select the kit you want to delete from the Calibration Kit or Performance Verification Kit lists. 3. Click Delete Kit. CAUTION: There is no confirmation dialog box when you delete a kit. Commands and Routines (Cmds & Routines) Tab Maintenance > Cmd & Routines Use this tab to create a routine or to edit, delete, or run a selected routine or command.
• Routine Name - This list of pre-set routines and commands can be used for system maintenance. Some of these commands are also available on the Auto Maint tab. You can also create custom routines which appear in the Routine Name list after you save the routine. To create a custom routine, start with None and add commands. You can also modify a Luminex routine and save it as a new routine.
WARNING: When using multiple plates, use them in the proper order. Failure to do so can result in inaccurate data and test results. Commands - This section contains the following commands: • Wash - Sends distilled water through the fluidic lines in the system. It pulls the fluid from a well or from the reservoir and runs it completely through the system to the waste receptacle. • Alcohol Flush - Removes air bubbles from the sample tubing and the cuvette using 70% isopropanol or 70% ethanol.
• Command Sequence - When you select a routine, the individual commands in the routine appear in order in the Command Sequence list. The list includes the name of each command, location, status, and any additional information. • Report - Opens the Reports tab with Calibration and Verification Reports selected in the Report: list. Click Generate to view the selected report. • Import - Displays the Open dialog box to select a routine file to import. Select a file and click Open to import it.
6. Click Save As to save the new routine. The Save Routine dialog box opens. 7. Type the routine name in the Routine Name box, then click OK. Editing a Routine 1. Open the Maintenance page, then open the Cmds & Routines tab. Click the routine that you want to edit from the Routine Name list. 2. Click a command in the Command Sequence list that you want to edit. Click Clear to delete the command, or click in a different well in the plate image to change the location of that command.
6. Click Run. The Routine Message dialog box opens when the routine is complete. 7. Click OK. Importing a Routine 1. Open the Maintenance page, then open the Cmds & Routines tab. 2. Click Import. 3. In the Open dialog box, browse for the file you want to import, then click Open. The routine becomes the active routine. Exporting a Routine 1. Open the Maintenance page, then open the Cmds & Routines tab. 2. Click Export. 3.
• Auto Adjust Height - Automatically adjusts the probe height using the specified location(s) in the main plate, reservoir, and strip wells. NOTE: Luminex recommends that you calibrate the probe height using well D6. • Plate Location pin - A green pin that shows the well location used to auto adjust the probe height for the main plate, reservoir, and/or strip wells. The default (and recommended) location for the main plate is well D6. • The main plate depicts a 96-well microtiter plate.
• Delete - Deletes the current plate from the available plates in the Plate Name list. • Save Plate - Appears when you have typed a new plate name in the Plate Name list, and is used to save the current plate. A warning message opens. NOTE: If you save a plate without performing an auto adjust, the plate name is saved, but the probe height adjustment is not. If you do this, you cannot use the plate with this instrument.
5. Click Eject to eject the plate carrier. 6. Place the off plate reagent block on the plate carrier. Make sure it is well seated so that it clips into place. 7. Place a strip well (provided with the Calibration and the Performance Verification kit) in the off-plate reagent block. 8. In the Strip Wells section, click SD1. 9. Verify that the reservoir is empty. 10. In the Reservoir section, click well RB1. 11. Verify that the plate is not warped. Warped plates can lead to incorrect probe height adjustment.
FIGURE 7. Sample Probe Height Adjustment System Info Tab Maintenance > System Info Use this tab to view information and diagnostics about the Luminex instrument.
• Operating System • Licensing • Instrument Type • Serial Number • Firmware Version • XYP Heater Temp • Calibration/Verification Status • Delta Calibration Temp • System Temperature • Last CAL Calibration • Last VER Verification • Last Fluidics Test • Drive Fluid • Waste Fluid Items in this list relating to calibration and verification have one of the following states: • Passed - Indicates that the process completed successfully. • Failed - Indicates that the process was not completed successfully.
Use this tab to view, print, and save system status log information. This tab contains the following: • Search By Log Type - Filters the type of log information. Select All, Maintenance, Security, or Warnings and Errors. • Search By Log Date - Enables using a date range to display a log of system activities. • Advanced - Displays the following added information in the system log: • Log Type • User ID • Error Level • Log - Displays a list of information about each system process.
Use this tab to view reminders for scheduled maintenance due to be performed on the analyzer. Reminders - This section displays reminders for scheduled maintenance. • Subject - The maintenance occasion scheduled for an alert. • Reminder - A description of the maintenance that you need to perform on that occasion. • Next Alert Date - The date on which xPONENT alerts you about the maintenance occasion. • Alert Time - The time at which xPONENT alerts you about the maintenance occasion.
Use this tab to create a support file that you can send to Luminex Technical Support. To include batch information in the support file, use the Include Batch Information button and the Select Batch table. • Include Batch Information - Select this to include batch infromation with the support file. It actives the Select Batch table.
3. Type your name in the Name box. 4. Type your company name in the Company box. 5. Type your phone number in the Phone box. 6. Type your email address in the Email box. 7. In the Comment box, type a detailed description about the problem you are experiencing. 8. Verify the location where you want to store the file. To change the location, click Browse, then navigate to the new folder and click OK. 9. Click Save File. The saved file includes date and time information. 10.
Chapter 7: Admin Page You must have the appropriate license to view some of the features of in the Admin page.
Use this tab to configure system settings such as application settings, LIS settings, maintenance page options, and external analysis program settings. Application Settings These settings change some of the basic ways that xPONENT works, allowing customization of many of the basic functions. • Enable Virtual Keyboard - Enables the touchscreen virtual keyboard. • Allow the application to be minimized - Enables minimization of the xPONENT software so that the computer’s desktop can be accessed.
• Browse - Opens another dialog box to select the file location for the third-party analysis program. The selected location appears in the Path box, and the name displays in the Name box. • Command Line Parameters - To keep the default command line settings, leave Command Line Parameters blank. Otherwise, type the command line parameter for which parameters you want xPONENT to use with the external analysis program.
1. If the program is on an external media such as a CD or flash drive, insert the media. 2. Click Add New to open the New External Analysis Program dialog box. 3. Type a name for the external analysis program. 4. Click Browse to navigate to the .exe file for that program. Double-click the file name. 5. Type the command line parameter for the parameters you want xPONENT to use with the external analysis program.
NOTE: See "Daily Activities" on the Home page for daily maintenance routines. Luminex recommends that you verify daily and calibrate weekly. You should also verify and calibrate if any of the following occurs: • The delta calibration temperature exceeds ± 5° C. • You move the instrument. • You experience sample acquisition problems. • You perform maintenance on the instrument, for example, replacing a hardware component.
3. Under Maintenance Options, select Fluidics prep, performance verification from the drop-down list. FIGURE 8. Setting System Initialization Routine 4. Click Save. Group Setup Tab Admin > Group Setup This tab is accessible only in the Security or 21 CFR Part 11 packages. Use this tab to assign permissions to different groups of users. Additionally, if you have the 21 CFR Part 11 package, you can require an electronic signature in order to perform selected tasks.
Users are assigned to groups and permissions are granted to each group. Do not assign permissions directly to individual users. Use this tab to unlock accounts for users who have locked themselves out of the system. Group Features - In this Group Profile list, select the group profile you want to set up. In the list, select the features you want to assign to the Group Profile you selected. Finally, Features are used to assign a specific feature to a group. To select a feature, select the check box.
• System Administration • Manage Users (add, edit, or delete users) • Manage System Configuration • Perform Calibration and Verification • Manage Alerts • Manage scheduled maintenance • Change batch options • Allow exit software • Batch run override system • Create, delete, activate Cal and Ver Lots and Kits • Batch Management • Create Batch • Edit Batch • Delete Batch • Run Batch • Validate and Invalidate Results • Replay Batch/Recalculate Data • Approve Batch • Reanalyze Results • Save Batch after changin
When you perform an action that requires an electronic signature, the Electronic Signature dialog box opens. The user ID autopopulates. Type your password and any comments. Click OK to complete the electronic signature, or Cancel to cancel the signature. • Cancel - Cancels changes. • Save - Saves changes. Setting Up Group Permissions 1. In the Group Profile list, click the group profile you want to set up. 2.
• Create New User - Opens the Create User Account screen. • Global User Settings - This section contains the following: • Password Expiration - Type a length of time in days. The default is 180 days. • Unsuccessful Login Attempts - The default number of allowed unsuccessful logins is three attempts. • Automatic Logoff (seconds) - How many seconds pass until an automatic logoff. Default is 0. • Minimum User ID Length - The default minimum User ID length is six characters.
Use this tab to set the options for a new user. Admin users can assign rights to any and all groups. Either the 21 CFR Part 11 or the Secure package is required. This tab contains the following: • User ID • User • Account Status check box • Group Profile list • Password • Reenter Password • Change password after next login - Requires the user to change the password after the first login. This check box is enabled by default. • Save - Saves and returns to User Setup.
On this page, users can modify the information of the user who was selected from the User Setup tab. The Edit User page contains the following: • User ID - The identification of the user. • Name - The user's name. • Group Profile - The user's group or category. • Change Password After Next Login - A box that, when checked, indicates that the user will change his or her password at the next login. • Account Status - Locked or functioning.
Editing User Permissions 1. In the Users list, click the user ID, then click Edit User. 2. In the Edit User Account window, edit the desired information. 3. Click Save. Define Global User Settings The settings in the Global User Settings section affect all users in all user groups. You can retain the default settings or type your own values. To define Global User Settings: 1. In the Global User Settings section, type a length of time (in days) for the Password Expiration. 2.
Use this tab to set options for analysis and acquisition of batches. This tab contains the following: xPONENT for MAGPIX 4.
• Batch Options - This section displays the following options: • Allow running or saving a batch with expired reagents and fluids - Allows batches to run with expired reagents and fluids. • Allow running a batch if XY temperature is not in range - Allows batches to run if the XY temperature is not in range. • Allow running a batch if the instrument is not calibrated or verified - Allows batches to run if the instrument is not calibrated.
This cannot be deleted or modified and runs by default. However, you can turn off this routine or use a different routine. Luminex recommends cleaning the system with 0.1N NaOH. • Default Analysis Graph Axes - Either Log X Axis or Log Y. • Batch Thresholds • Detection enabled - Enable or disable low bead count detection. • Well Count - When this number of consequetive wells acquire a bead count lower than the number specified in the Total Bead Count box, the low bead count action is taken.
This tab contains the following: Alert Options - Offers three check boxes for each alert - Dialog, Email, and Sound. This section displays the following events: • Locked User - The user is locked out of xPONENT. • Batch Complete - The batch is finished. • Heater In Range - The plate heater is warmed to the designated range. • Scheduled Maintenance - A scheduled maintenance routine is now due. • Low Bead Count Detected - A bead count below the accepted range is encountered.
The Test button tests the email settings. Select Apply to initiate the settings you’ve selected, or click OK to use the settings and exit the dialog box. Cancel exits the dialog box without saving the settings. Alert Options Tasks To set notification options for various events: 1. Select Dialog if you want a dialog box to open for a specific event. 2. Select Email if you want to send an email notification for a specific event. 3. Select Sound if you want a sound to play for a specific event. 4.
You must have administrative privileges to perform this task if you are using the 21 CFR Part 11 or Secure package. Use this tab to define what the CSV (comma-separated values) file will contain and where it will be stored. This tab contains the following: CSV and Batch Export Options • Automatically export results CSV file when batch is complete - Automatically export the results .CSV file when the system finishes analyzing the batch.
Archive Options Tab Admin > Archive Use this tab to archive all file types - batches, protocols, kits, lots, and LIS records. When you click the Launch Archive Utility button, the Archive Utility opens. NOTE: To back up or restore xPONENT files, quit the application and select XPONENT ARCHIVE from the Windows start menu. Archive Utility Admin > Archive > Archive Utility xPONENT for MAGPIX 4.
The Archive Utility can be used within xPONENT to set up frequency and type of files to back up. Statistics about disk space and archive space used, as well as the types of files to backup, appear on this page. The archive utility runs continuously. If automatic archives are scheduled, they occur whether xPONENT is running or not. When you want to backup or restore xPONENT, quit the application and run the Archive Utility from the Start menu.
• System Backup - Click this to back up your system. It requires a log in and then instructs you to shutdown the xPONENT software and launch the Archive Utility from the Start menu. • System Restore - Click this to restore your system. It requires a log in and then refers you to the Start menu to restore your system. • Manual Archive - Click this to perform a manual archive process. It requires a log in and then opens the Manual Archive window. • Close - Click this to close the Archive Utility.
Setting Up Automatic Archiving Click Edit in the Schedule Overview box to enable the fields to accept your edits and to enable the Browse button. The label on the button changes to Save. 1. Specify when you want your archives to begin using the calendar. 2. Using the drop-down list, select how often you want to perform archives. 3. Select Reminder Only to be reminded of the need to archive, after which you must archive manually. Clear this check box to enable automatic archives. 4.
1. In the Archive Utility, click Manual Archive to open the Manual Archive window. 2. Using the tabs on the left side of the window, select the type of files you want to archive: • Batches • Logs • Protocols • Kits • Lots • LIS Records Each tab opens a list of files available for archiving, except Logs, which requires only a choice of how old, in days, a file has to be to be archived. 3.
Contact Luminex Technical Support to upgrade xPONENT for MAGPIX or to obtain a new license. NOTE: You must restart the computer for the new license to take effect. Adding a New License Key Contact Luminex Technical Support if you have any difficulty saving or adding a new license key. 1. Access the Admin page, then the Licensing tab. 2. Click License (bottom right corner of window). 3. Copy and paste the new key into the License Code field. The License File field remains blank. 4. Click OK.
Use the Schedule tab to enable or change the recurrence of scheduled maintenance reminders. • Select the time of day that you want to receive alerts in the Alert Time list. • Select how often you want to receive reminders in the Recurrence list. • Enable or disable the reminders by selecting or clearing the Enabled check box. Editing Maintenance Schedule Settings Edit maintenance settings on the Schedule tab accessed from the Admin page.
1. Access the Admin page, then the Schedule tab. 2. On the Schedule tab, use the dropdown menus to edit the following items for any scheduled activity: • Alert Time - the time of day that you want to receive alerts. • Recurrence - how often you want to receive reminders. 3. Enable or disable the reminders by selecting or clearing the Enabled check box. Report Options Tab Admin > Report Options Use Report Options to set how reports are displayed and printed.
1. Type a company name in the Company box, and any additional information in the Info: box. NOTE: The logo file should be 920 x 125 pixels. If you want the logo to appear to the right of your company name, include 120 pixels of white space to the left of the logo, in the graphic file. If you do not include white space, the logo may appear behind the company information. 2. Click Import Logo to open the Open dialog box and select the file you want to use for the logo at the top of reports. 3.
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